Tim Broderick Email & Phone Number

Durham, North Carolina, Us

Durham, North Carolina, Us

Durham, North Carolina, Us

Brentford, Middlesex, Gb

• Support multiple Drug Discovery programs by generating / purifying / analyzing recombinant proteins and monoclonal antibodies needed for crystallography, assay development, Encoded Library Technology (ELT), or animal studies.• Upstream Process Development: optimize media, shake flask, and bioreactor conditions (batch and fed-batch) for E. coli, Pichia pastoris, SF9, CHO, and HEK293 expression systems• Downstream Process Development: Purify tagged or untagged proteins and monoclonal antibodies using AKTA FPLC with Affinity, SEC, IEX, HIC, or Hydroxyapatite columns.• Analytical QC: SDS-PAGE, Western blot, ELISA, BioRad, UV, HPLC, LC/MS, qPCR• Developed cGMP-compliant high cell density fermentation process for large-scale production of Lactobacillus plantarum.

Brentford, Middlesex, Gb

• Designed and expression tested novel expression plasmids for E. coli and Pichia pastoris• Optimized media and expression conditions of recombinant E. coli and Pichia pastoris in 10L B. Braun, 15L New Brunswick, and 75-L BioLafitte fermentors using low density batch and/or high cell density fed-batch fermentations.• Coordinated IQ, OQ, and PQ of four new SIP / CIP 30-L Applikon Bio Bench Bioreactors

Brentford, Middlesex, Gb

• Introduced and implemented Pichia pastoris expression system• Designed and expression tested novel expression plasmids for E. coli and Pichia pastoris• Optimized media and expression conditions of recombinant E. coli and Pichia pastoris in 10L B. Braun, 15L New Brunswick, and 75-L BioLafitte fermentors using low density batch and/or high cell density fed-batch fermentations.• Developed assays for analysis and characterization of protein products (SDS-PAGE, Western Blot, LC/MS, small-scale purification)

• Developed and validated new client E. coli, Pichia pastoris, and CHO fed-batch processes at 15L and 140L scale under non-GMP and GMP conditions for Phase I/II Early Stage Clinical products• Generated development reports and assisted in technology transfer to Phase III manufacturing• Assist Analytical and Purification Development groups with method development and validation

Chapel Hill, Nc, Us

• Assisted in the characterization of various MHC Class I/peptide complexes for structural and immunotherapeutic studies• Cloned alpha and beta chains of MHC Class I into E. coli expression vectors, optimized expression, purified inclusion bodies, refolded alpha and beta chains with synthetic peptides. Biotinylated MHC Class I/peptide complexes into tetramers with streptavidin for T cell studies

Mentor, Oh, Us

• Tested / qualified new production lots of Biological Indicators inoculated with Bacillus stearothermophilus in steam and vapor hydrogen peroxide (VHP) sterilizers• Tested / qualified new production lots of Biological Indicators inoculated with Bacillus subtilis in ethylene oxide and dry heat sterilizers• Determined bacterial population of Biological Indicators via serial dilution• Tested new lots of chemical indicators and autoclave tape• Performed Environmental Monitoring of Clean Rooms