Tim Broderick Email & Phone Number

Durham, North Carolina, US

Durham, North Carolina, US

Durham, North Carolina, US

Brentford, Middlesex, GB

• Support multiple Drug Discovery programs by generating / purifying / analyzing recombinant proteins and monoclonal antibodies needed for crystallography, assay development, Encoded Library Technology (ELT), or animal.
• Upstream Process Development: optimize media, shake flask, and bioreactor conditions (batch and fed-batch) for E. coli, Pichia pastoris, SF9, CHO, and HEK293 expression systems
• Downstream Process Development: Purify tagged or untagged proteins and monoclonal antibodies using AKTA FPLC with Affinity, SEC, IEX, HIC, or Hydroxyapatite columns.
• Analytical QC: SDS-PAGE, Western blot, ELISA, BioRad, UV, HPLC, LC/MS, qPCR
• Developed cGMP-compliant high cell density fermentation process for large-scale production of Lactobacillus plantarum.

Brentford, Middlesex, GB

• Designed and expression tested novel expression plasmids for E. coli and Pichia pastoris
• Optimized media and expression conditions of recombinant E. coli and Pichia pastoris in 10L B. Braun, 15L New Brunswick, and 75-L BioLafitte fermentors using low density batch and/or high cell density fed-batch.
• Coordinated IQ, OQ, and PQ of four new SIP / CIP 30-L Applikon Bio Bench Bioreactors

Brentford, Middlesex, GB

• Introduced and implemented Pichia pastoris expression system
• Designed and expression tested novel expression plasmids for E. coli and Pichia pastoris
• Optimized media and expression conditions of recombinant E. coli and Pichia pastoris in 10L B. Braun, 15L New Brunswick, and 75-L BioLafitte fermentors using low density batch and/or high cell density fed-batch.
• Developed assays for analysis and characterization of protein products (SDS-PAGE, Western Blot, LC/MS, small-scale purification)

• Developed and validated new client E. coli, Pichia pastoris, and CHO fed-batch processes at 15L and 140L scale under non-GMP and GMP conditions for Phase I/II Early Stage Clinical products
• Generated development reports and assisted in technology transfer to Phase III manufacturing
• Assist Analytical and Purification Development groups with method development and validation

Chapel Hill, NC, US

• Assisted in the characterization of various MHC Class I/peptide complexes for structural and immunotherapeutic studies
• Cloned alpha and beta chains of MHC Class I into E. coli expression vectors, optimized expression, purified inclusion bodies, refolded alpha and beta chains with synthetic peptides. Biotinylated MHC Class I/peptide.

Mentor, OH, US

• Tested / qualified new production lots of Biological Indicators inoculated with Bacillus stearothermophilus in steam and vapor hydrogen peroxide (VHP) sterilizers
• Tested / qualified new production lots of Biological Indicators inoculated with Bacillus subtilis in ethylene oxide and dry heat sterilizers
• Determined bacterial population of Biological Indicators via serial dilution
• Tested new lots of chemical indicators and autoclave tape
• Performed Environmental Monitoring of Clean Rooms