Tim Keating work email
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Hello!I have spent my professional career developing my passion for science into a robust set of skills which have a proven record of excellence. By combining meticulous detail oriented thinking, technical expertise, and innovative ideas; I have been able to solve problems, improve process, and deliver guidance across all aspects of early-stage discovery biologics production and characterization.Specifically, I have a mastered skill-set in the following areas:- Protein Expression/Fermentation across multiple cell lines (E.Coli, HEK293, CHO, and hybridoma) at many scales ranging from 1mL to 60L- Setup and Handling of TFF equipment both automated and manual operation.- Protein Capture and Cleanup with IMAC, HIC, AEX/CEX, Protein A/G/L, and other industry standard methods Utilizing AKTA instruments and unicorn 5/7.- Protein characterization across upstream and downstream process via SDS-PAGE, CE-SDS, DLS and Protein Solubility.- Operation of Agilent HPLC equipment/software in both reverse phase and standard SEC mode for aggregate measurements and stability- Operation of BLI (octet) equipment/software for both protein kinetics and titer measurement with extensive development time on both areas.- Waters LC-MS QTOF with UNIFI systems for reduced mass glycan analysis.- Technical Operations: Microsoft products, computer equipment, lab equipment. If something stops working I am generally the person asked to fix it.My career goal is to lead a team where I can leverage my leadership skills as well as my technical expertise to drive product innovation at the support or discovery stage. Patients need therapies, and passion at all levels makes this happen.Outside of my work life I have a very important job: I am a father and a husband. I also have many hobbies involving computer building/tinkering, cooking, exercising and the occasional book on astrophysics by Sagan or Tyson. While I am very driven in my career, balance between life and work is incredibly important to me.
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Senior Scientist IiAbbvie Dec 2023 - PresentNorth Chicago, Illinois, UsMSAT Downstream Team Leader -
Senior Scientist IAbbvie Jun 2022 - Dec 2023North Chicago, Illinois, UsLeading the Pilot Lab downstream team to deliver GLP Tox. and Rep. Batches of biologics at the 50L - 1000L scale with tight deadlines and challenging modalities.Leading multidisciplinary technical teams and driving successful campaigns from transfection to IND.Deliver GLP/GMP process development improvements, mentor junior level scientist on implementation strategies.Interrogate and resolve GLP/GMP process characterization and impurity challenges and present findings across the organization.Provide on floor support, person-in-plant, batch record drafting/review, and tech transfer. -
Scientist IiAbbvie Apr 2020 - Jun 2022North Chicago, Illinois, UsLeading GLP Tox and Pre-IND programs through the Scale-Up Lab to deliver representative batches of biologics at pilot scale.Acting as downstream lead in multidisciplinary technical team meetings.Interfacing with process development to drive downstream tech transfer, process fit, batch record drafting and review. -
Scientist IiAbbvie Jul 2017 - Apr 2020North Chicago, Illinois, UsManaging the transient expression tissue culture lab of the Core Biochemistry group in early discovery.Responsible for internal review of product QC before release.Investigating glycan distribution using LC-MS.Responsible for large scale in house purification and characterization of early discovery biologics (5-25L).Exploring cell lines/ expression methods for product yield, glycan structure, and protein stability. -
Scientist IAbbvie Jul 2016 - Jul 2017North Chicago, Illinois, UsSupporting the core discovery group via the expression, purification, and characterization of a large number antibody isotypes and formats.Optimized current tissue culture protocols to increase consistency, product titers, and protein recovery.Optimized and standardized the use of Octet for accurately determining tissue culture titers.Utilized protein A/G and AKTA systems to purify a wide range of antibody formats , and used HPLC SEC, LAL Endoxin Assay, SDS PAGE and UV analysis for characterization.Assisted in the optimization and standardization of current purification protocols to increase product yield and quality.Analytical support for early stage discovery projects. -
Research Associate IiOmniox Inc Sep 2015 - Jul 2016 -
Research AssociateOmniox Inc Jan 2013 - Sep 2015Assisting in the biochemical development and production of an oxygen carrying protein therapy into clinical trials.Key Accomplishments:-Developed a number of small scale assays/purification schemes to test and verify protein expression.-Developed an assay which monitored protein solubility across upstream production.-Worked as the primary analytics during fermentation process development with a CMO.-Developed the upstream scale up of 12L to 60L in house production a day.-Developed various protocols and protocol improvements to current processes.Experience:-Knowledgeable of bacterial expression systems and quick ways to check protein expression.-Set up/ Operation of TFF units, including plate based membranes and hollow fiber membranes.-Knowledgeable of the expectations of GMP.-Bacterial culture.-Protein purification.-Assay development.-QC/QA.-PCR and Molecular Biology (cloning, etc).-Elisa.-Western Blot. -
Research AssistantUcsf Oct 2010 - May 2012San Francisco, California, UsWorked directly with PI to study the effect of specific site mutations on the AMPA receptor, to learn more about the biophysical process of its operation.Key Accomplishments:-Created 19 mutant constructs to be used for study by the lab.-Optimized various protocolsResponsibilities: -PCR Mutagenesis-Cell Culture-Cloning-mRNA work, specifically transcription and microinjection-Animal surgery and post surgery careAdministrative Responsibilities-Work with regulatory departments to demonstrate that current protocols and regulations are being followed.-Handle appropriate training of any new lab members in any of my general responsibilities.-Maintain accurate records of all transactions and procedures.
Tim Keating Skills
Tim Keating Education Details
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University Of California, Santa CruzNeuroscience And Behavior
Frequently Asked Questions about Tim Keating
What company does Tim Keating work for?
Tim Keating works for Abbvie
What is Tim Keating's role at the current company?
Tim Keating's current role is Senior Scientist II at AbbVie.
What is Tim Keating's email address?
Tim Keating's email address is ne****@****ail.com
What is Tim Keating's direct phone number?
Tim Keating's direct phone number is +141543*****
What schools did Tim Keating attend?
Tim Keating attended University Of California, Santa Cruz.
What skills is Tim Keating known for?
Tim Keating has skills like Cell Culture, Protein Expression, Molecular Biology, Protein Purification, Assay Development, High Performance Liquid Chromatography, Sds Page, Elisa, Biochemistry, Data Analysis, Liquid Chromatography Mass Spectrometry, Design Of Experiments.
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