Tim Krueger Email and Phone Number
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I am driven to help patients through research. Over 10 years of work in oncology and immunology, I have developed numerous diverse approaches to cancer therapy, including the examples below and further undisclosed projects.• Used epigenetic modulating agents to upregulate cancer-testis antigens (CTAs) and antigen processing machinery, increasing efficacy of CTA-based vaccines. • A small molecule LSD1 inhibitor targeted hormone/growth signaling as well as cells of the TME, including pro-inflammatory M1 macrophage polarization. A PROTAC variant led to targeted protein degradation.• Depleted anti-inflammatory cancer-associated fibroblasts with prodrugs activated by the protease activity of fibroblast activation protein.• Used IV-infused mesenchymal stem cells to deliver internalized cytotoxic agents.• Inhibited anti-inflammatory IL-4/IL-13 signaling on macrophages.• Inhibited pro-tumorigenic miRNAs.I also have 2 years of experience in other immunological disorders such as EAE (MS), lupus, arthritis, and allergy. I have done immunophenotyping and functional assays with innate and adaptive immune cells including NK cells, T cells, Tregs, B cells, plasmablasts, monocytes/macrophages, conventional and plasmacytoid DCs, neutrophils, and fibrocytes. I am proficient with mouse tissues (syngeneic/xenograft models, primary cells, cell lines) and human tissues (PBMCs, whole blood, cell lines).Over the course of such projects, I gained extensive drug development experience in areas such as target validation, in vitro assay development, in vivo xenograft/syngeneic mouse models, biomarker discovery, and pre-clinical PK/PD with a proven track record of success. Over 7 years of undergraduate/graduate research, I earned authorship on 14 publications and drove a project to an upcoming clinical trial. In the industrial setting, I have completed assay development for 4 projects entering clinical trials, including report writing for IND submission.Conventional and spectral cytometry is my greatest technical strength, featuring 8 years of experience across 5 instruments. I have performed numerous mouse immunophenotyping studies, including dissection of tissues (tumor, spleen, lymph nodes, bone marrow, thymus) and training of 7+ people across 5 different laboratories. At CellCarta, I led an 8-person team to develop deterministic, data-driven approaches for effective panel design and used these approaches to create novel large (up to 35c) modular panels, a pivotal part of the company’s push into spectral flow cytometry services.
Ngm Biopharmaceuticals
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- ngmbio.com
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Scientist I, Immune OncologyNgm BiopharmaceuticalsSan Jose, Ca, Us
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Scientist, R&DCellcarta Jan 2023 - Jul 2024Montreal, Quebec, Ca• Acted as the principal scientist on six biologically diverse, client-based human immunology projects utilizing cytometry (conventional, spectral, or mass). Independently handled all aspect of these projects, including experimental design and planning, hands-on work, data analysis and presentation, and client communication.• Completed assay development to support clinical trials across three different companies, including report writing for IND submission.• Handled project management duties including budget tracking, sample tracking, and setting of timelines.• Earned accolades from clients for clear and prompt verbal and written communication, ability to troubleshoot, insightful suggestions, critical review of literature, flexibility in approach, high-quality data and visualizations, and ability to make decisive conclusions. Received an unprompted job offer from a client.• Mentored three RAs/SRAs on experimental design principles and flow cytometry techniques.• Independently initiated several significant internal projects for company process-improvement, the largest of which was an 8-person team.• Developed a deterministic, data-driven approach for cytometry panel design that was used to create novel modular panels, a pivotal part of the company’s recent push into spectral flow cytometry services. -
Scientist, ImmunologyNgm Biopharmaceuticals Nov 2021 - Dec 2022South San Francisco, Ca, Us• Gained broad drug development experience via three projects that ranged in stage from conception to clinical translation and in disease models from autoimmunity to cancer immunology.• Designed and performed biochemical and immune assays to assess compound activity and determine mechanism of action in human cell-based (cell lines and primary cells) and in vivo mouse studies.• Designed and optimized a series of panels for deep immunophenotyping of mouse and human tissues using 27-35 color spectral cytometry.• Participated in a “think tank” group for new project ideation.
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Graduate Student, Labs Of John Isaacs And Nathaniel BrennenThe Johns Hopkins University School Of Medicine Nov 2016 - Nov 2021Baltimore, Md, Us• Spearheaded a highly collaborative project between our academic lab and the CEO and scientists of Imago Biosciences to develop and characterize a novel inhibitor of an epigenetic enzyme, LSD1.• Tested the hypothesis that pharmacologic and genetic inhibition of LSD1 can be effective against lethal castration-resistant prostate cancer independent of androgen receptor signaling by using 10+ syngeneic or xenograft mouse models, including patient-derived xenografts and genetically-modified (CRISPR, shRNA) cell lines.• Used ex vivo and 3D culturing experiments to demonstrate direct effects of LSD1 inhibition on cells of the tumor microenvironment, including inhibition of endothelial sprouting and promotion of M1 macrophage polarization.• Designed and optimized six 8-12 color flow cytometry antibody panels used to identify lymphocyte, myeloid, and stromal populations in large mouse experiments. Trained 5+ people to perform tissue collection (tumor, spleen, lymph nodes, bone marrow, thymus), antibody staining, and data analysis. 4-5 collaborative publications are expected resulting from this work.• Developed a protocol for isolating and expanding murine bone marrow-derived mesenchymal stem cells (MSCs).• Utilized MSCs as delivery vehicles for cytotoxic agents to tumors. Longitudinally tracked homing of IV-infused, 89Zr-labeled human MSCs in mice using µPET imaging.• Performed and trained others to perform ex vivo human T-cell co-culture experiments to functionally analyze T-cell growth and activation.• Chosen as the 2019 Johns Hopkins applicant for the PhRMA Foundation Predoctoral Fellowship, and was then selected among national applicants to receive a two-year, $40,000 fellowship.• Demonstrated desire for knowledge and self-improvement by taking one elective course each semester of the final three years during my thesis work.• Gained extensive cancer cell line culture experience, including viability, clonogenic, and cell cycle assays. -
Research Associate, Lab Of Gerard EvanUniversity Of Cambridge Jul 2016 - Aug 2016Cambridge, England, Gb• Selected for the SCORE biochemistry summer research program at the University of Cambridge. • Received more than complete funding through three scholarships, including the Promega International Scientific Internship Scholarship. Presented findings at Promega Corporate Headquarters.• Studied the molecular consequences of varying levels of mutated KRAS expression and regularly performed western blot, immunohistochemistry, and proliferation assays using primary mouse embryonic fibroblasts (MEFs) isolated from transgenic mice. -
Undergraduate Researcher, Lab Of Joshua LangUniversity Of Wisconsin-Madison Feb 2013 - Jun 2016Madison, Wi, Us• Collaborated with labs within and outside of UW Madison to establish a project using an automated microfluidics platform for the isolation of methylated DNA from low input samples to enable robust and scalable biomarker approaches.• Used epigenetic modulating agents to upregulate cancer/testis antigens and antigen processing/presentation machinery in prostate cancer models for combinatorial synthetic lethality immunotherapy approaches.• Analyzed numerous cell lines and patient samples for gene expression by qRT-PCR.• Gained communication skills by presenting 10+ research presentations and several research posters, including at two national meetings. -
Laboratory AssistantUniversity Of Wisconsin-Madison Aug 2012 - Feb 2013Madison, Wi, Us• Trained five undergraduate students in lab management and research duties.• Demonstrated resourcefulness by creating novel databases for tracking orders and samples.
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Research Intern, Lab Of John DenuUniversity Of Wisconsin-Madison Jun 2011 - Sep 2011Madison, Wi, Us• Developed a novel continuous fluorescent assay to measure histone acetyltransferase (HAT) activity, which was then used to perform enzyme kinetics assays in the presence of HAT inhibitors.• Competed in the Capital Science and Engineering Fair poster session, winning one award.
Tim Krueger Skills
Tim Krueger Education Details
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The Johns Hopkins University School Of MedicinePharmacology And Molecular Sciences -
University Of Wisconsin-MadisonBiochemistry With Emphasis On Molecular Biology
Frequently Asked Questions about Tim Krueger
What company does Tim Krueger work for?
Tim Krueger works for Ngm Biopharmaceuticals
What is Tim Krueger's role at the current company?
Tim Krueger's current role is Scientist I, Immune Oncology.
What is Tim Krueger's email address?
Tim Krueger's email address is tk****@****bio.com
What schools did Tim Krueger attend?
Tim Krueger attended The Johns Hopkins University School Of Medicine, University Of Wisconsin-Madison.
What skills is Tim Krueger known for?
Tim Krueger has skills like Cell Culture, Biochemistry, Epigenetics, Pcr, Qpcr, Molecular Cloning, Bisulfite Genomic Sequencing, Rna Isolation, Data Analysis, Filemaker, Graphpad Prism, French.
Who are Tim Krueger's colleagues?
Tim Krueger's colleagues are Chen Su, Adrien L., Helen (Chenxiu) Liu, Amir M. Ashique, Pastor Paul, Zimple M., Michael Chen.
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