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• A self-motivated scientific researcher who holds a Master of Science in Biomedical Laboratory Science/Biology from San Francisco State University and recently completed a Master of Science in Genetics, Genomics, and Bioinformatics from the University of California, Riverside with working experience as Research Associate/ Research Assistant.• Experiences include extraction of nucleic acid from various tissue specimen( blood, FFPE, urine) and ran clinical samples( blood, urine) using a specialized Q-PCR urine biomarker assay to monitor acute rejection or injury using QuantStudio 6 Flex platform and microfluidic Fluidigm assay platform. Worked on Q-PCR assays to validate and optimize the blood-based kSORT and urine biomarkers assays for monitoring of acute rejection to the market.• Analyzed data using Excel, R statistical program, and GraphPads. Knowledge of C/C++, PERL, Python, Java, Unix, R Project for Statistical Computing, MS Word, MS Excel, MS PowerPoint, Outlook, Window 95/98/00, NT, XP, Adobe Acrobat, SIMS, SNPedia, NCBI, dbSNP, Pubmed, Gene Ontology, Entrez, ExPASy, MAPPFINDER. Experienced in genomic, proteomic, and SNPs data analysis using NCBI dbSNP, SNPedia, OMIM, Pubmed, Expasy, and MappFinder tools.• Skilled with Microfluidic Fluidigm Assay, Digital PCR, Affymetrix Genome-Wide Human SNP genotyping 5.0/6.0 microarray assays, GeneChip expression 3’ amplification one cycle and two cycle cDNA synthesis and cRNA endlabeling microarray assays, DNA/RNA extractions, DNA/RNA purifications, QPCR, cDNA and cRNA amplification, Laser Capture Microdissection (LCM), Next generation sequencing library prep, ELISA, Immunoprecipitation, Kinase activity assays, Western Blotting and antibodies labeling, Mammalian Cell Culturing, In-Vivo and In-Vitro skills, Mouse Cytokine Protein Array, Mass Spectrometry ( SELDI, MALDI-TOF), Spectrophotometry, Microscopy Financial Services
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Research AssociateMultispan, Inc.San Jose, Ca, Us -
Senior Research AssociateMultispan, Inc. Feb 2024 - PresentHayward, California, UsPlan and setup assays for Customer Compounds profiling projects.Responsible for day to day operations in the lab. -
Research AssociateMultispan, Inc. Mar 2020 - PresentHayward, California, UsRun Cell based assays on numerous GPCR cell lines for functional analysis and quality assurance.Perform Flow cytometry assays for QC analysis.Run RT- PCR and CRISPR assays for gene alteration detection.Perform sequence alignment. -
Associate ScientistDiacarta, Inc. Jul 2016 - Apr 2018Pleasanton, California, UsSupport the development of NGS platform for mutation detection in cancer.Extraction, QPCR, library prep for NGS project.Process and analyze NGS data using the latest bioinformatics tools.Developed bioinformatics pipeline for analyzing sequencing data from (FASTQ-VCF-Clinical Report) on Targeted Pan Cancer Panel assay for detecting somatic and germline variants.QC and analysis of sequencing data using open-source and commercialize NGS tool packages(Qiagen Biomedical Genomic Workbench, QCI, BWA, samtools, GATK, etc). -
Research AssociateImmucor Sep 2014 - Jun 2016Norcross, Ga, Us• Worked on Q-PCR to validate and optimize the blood kSORT and urine biomarkers assays for monitoring of acute rejection to the market.• Process and ran clinical samples( blood, urine) using a specialized Q-PCR urine biomarker assay to monitor acute rejection or injury. • Experience working with digital PCR for cell free DNA project.• Analyzed data using Excel, R statistical program, and GraphPads. • Write small scripts in R to develop an application for the QPCR urine clinical assays. -
Graduate Student ResearcherUniversity Of California, Riverside Jul 2012 - Sep 2014Riverside, Ca, UsGraduate Student Researcher in Genetic,Genomic, and Bioinformatic at the University of California, Riverside. • Graduate Student in Nugent Lab, UCR• Work on project associated with telomere stability in the yeast model S. Cerevisiae.• Experienced with basic molecular biology and genetics. -
Research AssociateCalifornia Pacific Medical Center Research Institute Jan 2012 - Aug 2012San Francisco, UsHelp conduct research on biomarker discovery for kidney transplantation.Conduct QPCR and Fluidigm reactions for biomarker discovery development. -
Research AssistantStanford University School Of Medicine Jun 2010 - Dec 2011Palo Alto, Ca, UsPerform q-PCR using Applied Biosystem and Fluidigm platforms to determine biomarker genes on renal transplanted patients.Perform Elisa validations of biomarker genes on renal transplanted patients samples.Perform protoarrray to determine biomarkers on renal transplanted samples.Process biopsy samples on kidnesy transplanted patients.Perform routine molecular biology to extract total RNA, cDNA synthesis, and gene expression assaysAnalyze q-PCR data and other data in the lab. -
Research Associate IiIllumina Oct 2009 - May 2010San Diego, Ca, UsPerformed bead-based chip PCR for next generation sequencing.Testing chip PCR product development reagents and assays. -
Research AssociateParallel Synthesis Tech, Inc Jun 2009 - Aug 2009Perform PCR reaction in solution and on the parallume optical encoded beads.Perform various chemical assays, such as nonspecific oligos DNA binding experiment and gel electrophoresis on optical encoded beads.Perform coating of glass beads with silica and parallume encoded materials.Prepare weekly reports for the research findings in the lab.
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Research Assistant/AssociateAffymetrix, Inc Mar 2009 - Jun 2009Santa Clara, California, UsContribute to the development of assay and array products to expand the application of Affymetrix Genechip technology in expression analysis.Perform targets preparation for the GeneTitan microarrays and cartridge arrays.Perform cDNA synthesis, cDNA amplification, purification, fragmentation, PCR, and array-hybridization on Affymetrix platform.Perform DNA targets quantification and quality control using Nanodrop and Bioanalyzer. -
Research Associate, Research And Product DevelopmentBiomarker Pharmaceuticals Oct 2004 - Feb 2009Us• Performed SNPs genotyping assays using Affymetrix platform technology to associate polymorphic variations with their diseases.• Assisted in the development of a product GeneEssence for SNPs genotyping.• Conducted gene expression microarray assays such as cDNA and cRNA amplification, cRNA fragmentation, and cRNA biotin-target labeling to study differential gene expression in mice fed with experimental and control diets.• Conducted molecular biology technique, such as DNA and RNA isolation, purification, globin reduction, digestion, ligation, PCR, fragmentation, and end labeling.• Optimized and troubleshooted the development of the Affymetrix Genome-Wide Human SNP Array 6.0 genotyping assay.• Carried out RNA quantitative and qualitative analysis by using the ND-1000 Spectrophotometer Nanodrop and Agilent 2100 Bioanalyzer. -
Research Associate I, Applied ResearchIntermune, Inc Oct 2002 - Oct 2004Performed literature search for SNPs and their associated disorders through public databases.Revised and updated SOPs for research operations.; Performed Western Blot to examine protein expression level in response to drug treatment.Performed SDS-PAGE gel to separate and characterized proteins of interest.Performed cell culture techniques for the maintainance of adherent, epithelial mammalian cell lines including A549, OvCar, and Caco-2 carcinoma cells. Performed ELISA assays to quantify the TIMP1, MMP1, MMP2, and MMP9 to support idiopathic pulmonary fibrosis process.Performed cell proliferation and cytotoxicity assays on the effects of drug treatments for a variety of human cell lines.Performed protein concentration such as the Bradford Assay for the determination of the protein of interest.Ordered general lab supplies including reagents, media, and chemicals.Performed scientific presentation on projects conducted in the lab. -
Research AssociateNuvelo, Inc (Formerly Hyseq, Inc.) Mar 1998 - Oct 2002Performed high-throughput screening of clones using Genetix Q-fill, Q-pix, and Megapix robots to meet mass production quotas.Operated the Beckman Biomek 1000 Series to retrieve cDNA clones into 384 microtiter plates to ensure the analysis of specific genes.Prepared media, broths, and antibiotic stock solutions for daily operation.Performed gel electrophoresis and run various DNA samples on agarose gels to meet the the quality control operation schedule.Plate E'coli transformants into selective agar for screening purposes.Performed quality control PCR on E'coli clones arrayed in the lab.Assisted in the revision of SOPs to improve production quality.Assisted in the supervision of routine operations in the laboratory.Performed cell culture such as preparing media, checking cells, splitting cells, and harvesting cells for special projects. -
Clinical Manufacturing AssociateBiosource Technical Service May 2000 - Aug 2000Hand-filled drug powder into caplets in a cGMP environment to meet manufacturing demands before deadlines.Cleaned, maintained, assembled, and operated isolated containment equipments in a cGMP environment to satisfy production needs.Collected, maintained, and interpreted data into Excel as well as revised and updated SOPs to support manufacturing operations
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Lab TechnicianUsda, Ars Jun 1997 - Feb 1998Washington, Dc, UsIsolated the fungus Phytophthora ramorum into selective media for research studies.Performed various aseptic techniques to isolate and culture these fungi.Prepared media and agar for selective culture of fungus.Collected soil samples in the agricultural fields to analyze in the lab.
Tim Q. Tran Skills
Tim Q. Tran Education Details
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University Of California, RiversideAnd Bioinformatics -
San Francisco State UniversityBiomedical Laboratory Science/Biology -
University Of California, DavisMicrobiology
Frequently Asked Questions about Tim Q. Tran
What company does Tim Q. Tran work for?
Tim Q. Tran works for Multispan, Inc.
What is Tim Q. Tran's role at the current company?
Tim Q. Tran's current role is Research Associate.
What is Tim Q. Tran's email address?
Tim Q. Tran's email address is tq****@****ail.com
What is Tim Q. Tran's direct phone number?
Tim Q. Tran's direct phone number is +140896*****
What schools did Tim Q. Tran attend?
Tim Q. Tran attended University Of California, Riverside, San Francisco State University, University Of California, Davis.
What skills is Tim Q. Tran known for?
Tim Q. Tran has skills like Dna, Molecular Biology, Pcr, Assay Development, Cell Culture, Polymerase Chain Reaction, Laboratory, Immunology, Nucleic Acid Extraction, Rna, Elisa, Genomics.
Who are Tim Q. Tran's colleagues?
Tim Q. Tran's colleagues are Lisa Minor, Peter Hawryluk, Jennifer Pham, Renu Singh, Jared Peterson, Annie C..
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