Research Scientist
Merck & Co. Rahway, New Jersey
Small Scale Purification Operations:-Provided timely small scale (250ml-2L) purification of therapeutic proteins via primary recovery (centrifugation), IMAC, and Protein A chromatography and supplied material to Basic Research. -Used Millipore labtop TFF, AKTA Explorer and Purifier systems. Extensive experience with method writing and development using GE Akta chromatography systems.Large Scale Purification Operations:- Primary recovery and purification of monoclonal antibody projects in the Rahway Fermentation Pilot Plant. Included process transfer from the purification development group. - Support Safety assessment and material generation batches at 600-800L scale- Unit operations consisted of column chromatography, centrifugation, homogenization, depth filtration, microfiltration and ultrafiltration.Upstream Operations Support:- Cross-train in Fermentation and Cell Culture area- Assisted with 30L and 300L cell culture batches- Ran off-line analytical methods- Ran Transient transfection platform with dual plasmids in shake flasks, 2L and 20L wave bioreactors. - Authored 20L wave bioreactor procedures to assist new users and support outsourcing partners.800L Scale Purification Support: - Led retrofit of FPP UF/MF skid and AKTA Chromatography system.-Authored batch sheet for MF/UF steps and Chromatography skid to support safety assessment and material generation campaigns.- extensive knowledge of chromatography method development- process transfer lead with Purification development group