Timothy Fiore Email & Phone Number

Boston, Ma, Us

Boston, Ma, Us

Cambridge, Massachusetts, Us

Cambridge, Massachusetts, Us

Cambridge, Massachusetts, Us

-Leverage experience in bioprocessing of biologics to characterize novel pre-clinical candidate AAV vectors-Determine critical quality attributes of all pre-clinical grade vectors to ensure availability for in vivo studies-Evaluate and introduce new technology for analytical development-Identify and apply effective resolutions to any technical challenges during upstream and downstream processes-Contribute to the optimization of protocols for characterization of rAAV vectors using triple transfection and baculovirus/sf9 systems, especially for novel vectors-Ensure adequate use of electronic tracking system for documenting and tracking vector production requests, status and inventory

Optimize qPCR assays for molecular diagnostic device testing. Maintain laboratory records, prepare reports and define strategy for testing and optimization. Create new internal processes of testing, data collection, data acquisition, and data analysis. Exercise technical judgment on test requirements, developing recommendations and translating into course of action.

Cambridge, Massachusetts, Us

Collaborated with R&D team to design novel personalized neoantigen cancer immunotherapies. Responsible for molecular cloning, RNA synthesis, sequencing library preparation, PCR, and qRT-PCR. Conducted experiments to validate data from previous studies and optimize tumor antigen screening assay. Verified mutations in various cell lines by RNA-Seq. Validated gene of interest by molecular cloning, in-vitro transcription and sequencing.

Nutley, Nj, Us

Operated within the Translational Biomedicine Group. Performed assigned laboratory experiments. Followed standard experimental protocols; DNA/RNA library preparation and QC for NGS. Performed DNA/RNA isolation methods. Performed other molecular biology techniques, including qPCR. Identified inefficiencies in the work flow processes and developed a macro analysis template to increase productivity.Contributed to both drug discovery and clinical projects in a highly collaborative and team environment. Presented data at team project meetings.

Boston, Ma, Us

Worked in the Laboratory for Molecular Medicine (LMM) which is a CLIA-certified clinical diagnostic laboratory operating within Partners HealthCare Personalized Medicine. My duties within the lab include extracting and quantifying DNA from blood and other tissue samples, perform PCR and DNA sequencing reactions, use robotics to perform repetitive laboratory techniques, learn how to use new instruments and implement their use in new tests, participate in lab meetings and generate ideas for improving efficiency and productivity in the lab’s operations, optimize diagnostic tests and update existing standard operating protocols with significant guidance, follow existing protocols and regulations to QC equipment and reagents, enter requisition information and other report related data into a computer system, use universal safety precautions and proper personal protective equipment to protect self and co-workers from biohazardous materials, including blood-borne pathogens, comply with biohazard/radiation safety standards through proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace, complete annual biohazard/universal precaution safety training, as appropriate, perform various laboratory techniques involved in Next Generation Sequencing (NGS).

Boston, Ma, Us

Reported to Principal Investigator under research project: “Beverages and Societal Health.” Recruited, screened and scheduled potential participants to the study. Consented potential participants to research study. Coordinated visits with hospital staff and study subjects at CTSU in main hospital. Managed and developed database, entered data, and filed Case Report Forms (CRF).

Richmond, Va, Us

Reported to Principal Investigator (PI) whose research project involved multisensory properties of the Ferret brain. Examined ferret brain tissue using a PC-driven digitized light microscope controlled by Neurolucida software. Traced tissue outline, gray/white matter border, locations of labeled neurons and plotted over a million axonal boutons which were statistically analyzed and interpreted. Author and contributor of research presented at the 2011 Society for Neuroscience (November 12, 2011).

Richmond, Va, Us

Awarded the Summer Fellowship from the Undergraduate Research Opportunity Program. Presented research at the VCU Annual Poster Symposium. Funds allowed me to further my research for my Independent Study.

Charlottesville, Va, Us

Performed data entry and labeling of specimens accurately and timely. Prepared all specimens received for testing in designated laboratory departments such as staging of specimens, centrifugation, separate serum, proper storage, and aliquot samples. Resolved any problem specimens and effectively communicated with medical personnel accordingly.

Responded to fire and medical emergencies. Provided patient care for medical and traumatic injuries. Performed rescue operations, fire suppression, vehicle extrications, and traffic control. Provided training to new staff members, community service, and public education.

Minneapolis, Mn, Us

Assisted customers with any needs. Answered any questions about electronic devices (TV's, computers, audio, game consoles, computer software etc.). Restocked items, and merchandise label processing.

Bachelor Of Science (B.S.), Biology

Associate Of Arts (A.A.), Liberal Arts

Certificate Of Firefighting I And Emergency Medical Technician- Basic, Emergency Services