Dec 2024 - Present
Who is Tim Harding? Overview
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Quick answer
Tim Harding is listed as Scientist at Pfizer, based in San Diego, California, United States. AeroLeads shows a work email signal at pfizer.com and a matched LinkedIn profile for Tim Harding.
Tim Harding previously worked as Scientist at Freelance and Scientist III at Thermo Fisher Scientific. Tim Harding holds M.S., Molecular Pathology from Uc San Diego School Of Medicine.
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Email format at Pfizer
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{first}.{last}@pfizer.com
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Profile bio
About Tim Harding
Experienced Research Scientist with 20+ years specializing in Oncology and Immuno-Oncology therapeutic development. Expertise spans small molecule screening, gene manipulation, and cancer cell signal transduction. Skilled in preclinical discovery, GLP-compliant sample analysis, biomarker selection, and target validation. Proven track record in primary cell culture, immune cell isolation, and liquid tumor propagation. Adept at translating complex scientific insights into actionable therapies.Expertise includes biochemical characterization of tumor proteins and nucleic acids, cell lines and primary cells using multi-parameter flow cytometry, western blotting; molecular biology-- RT-qPCR, nucleic acid purification, gene manipulation by CRISPR/cas9, RNA interference, and viral transduction/transfection of genes using AAV, Lenti, Adeno and Retroviral vectors. Competent to BSL-III containment safety.Authorship of academic research, SOPs, Analytical Reports and support of IND filings. Oral primary data summaries, presentations at scientific meetings.Product development under GMP guidelines and knowledge of GMP standards, along with QbD concepts.
Listed skills include Cell Biology, Drug Discovery, Biotechnology, Oncology, and 25 others.
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Tim Harding's current company
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10 roles
Tim Harding work experience
A career timeline built from the work history available for this profile.
Scientist
Freelance
During my career transition, I have dedicated my time to enhancing my knowledge and skills in oncology research and drug development. My activities include:--Currently Enrolled in Online Curriculum: "An R Package for Dose-Response Data Analysis Using Logistic Models:"--Webinars in Oncology: Participated in several oncology webinars to stay updated on the.
Aug 2023 - Dec 2024
Scientist Iii
-Led the screening of small molecules and proteomic libraries via CRISPR/Cas9 knockouts to identify essential proteins for AAV titer enhancement.-Developed and optimized gene expression enhancers, including AAV viral particle production, through novel high-throughput assays in both adherent and suspension cell cultures.-Regularly applied flow cytometry.
Dec 2021 - Jun 2023
Scientific Researcher
Dec 2021 - Jun 2023
Scientist
Cytokine/Receptor modulation of immune function in GI related disease.Utilized multi-parameter flow cytometry for immunophenotyping human and non-human primate leukocytes from models of Irritable Bowel Syndrome (IBS) and Crohn's disease. Demonstrated development of Flow based cytokine receptor binding assays in human and non-human primate.
Aug 2021 - Nov 2021
Biology Scientist
Cancer Vaccines and Immunotherapy Group (ORD). Curated and managed a dynamic portfolio of over 20 concurrent immune/non immune cell lines, serving the demands of the CVIT department. Delivered biochemical support in the development of bi-specific antibody modulation in cancer.-Development and characterization of BCMA cell targets for bi-specific vaccine.
Jul 2019 - Aug 2021
Associate Scientist Ii
Ignyta, Inc.
Associate Scientist II, Translational Oncology.Biochemical analysis of the signal transduction pathways stimulated by the small molecule Entrectinib, aNTRK, Ros1, and ALK inhibitor, in the context of specific gene rearrangements and tumor resistance in cancer. -Utilization of 3D models of cancer engineered cell lines for proliferation assays as well as.
Jun 2016 - 2017
Associate Scientist
Celgene/Bristol Myers Squibb
Associate Scientist, Discovery OncologySmall Molecule Drug development including target validation and biomarker development in reference to non small cell lung and pancreatic cancers. MOA studies of small molecule inhibitors by construction of cell lines ( 3D culture), transfection of DNA/sh/siRNAs and viral infection.Experienced in phenotypic and.
Jul 2009 - Feb 2016
Senior Associate Scientist
Preclinical and clinical support for ANA773, a toll-like receptor 7 (TLR7) agonist, being developed for the treatment of cancer and hepatitis C virus (HCV) infection.Extraction of PBMC from both human and cyno whole blood for compound characterization by flow cytometry.Immunophenotyping of TLR-7 agonist dosed patients in phase II trials, and agonist dosing.
Feb 2006 - Jun 2009
Research Technician Iii
Investigated receptor and non-receptor tyrosine kinase signaling. Characterized the focal adhesion kinase homolog CADTK (Pyk2) signaling events and localized the protein to focal adhesions in smooth muscle cells. Compared and contrasted the signaling mechanisms of PYK2 from FAK from integrins and src family kinases and integrins.Studied signaling events.
Jun 1996 - Dec 2000
2 education records
Tim Harding education
M.S., Molecular Pathology
Uc San Diego School Of Medicine
Bachelor Of Science (B.S.), Biology/Biological Sciences, General
University Of Georgia, Athens, Ga
FAQ
Frequently asked questions about Tim Harding
Quick answers generated from the profile data available on this page.
What company does Tim Harding work for?
Tim Harding works for Pfizer.
What is Tim Harding's role at Pfizer?
Tim Harding is listed as Scientist at Pfizer.
What is Tim Harding's email address?
AeroLeads has found 1 work email signal at @pfizer.com for Tim Harding at Pfizer.
Where is Tim Harding based?
Tim Harding is based in San Diego, California, United States while working with Pfizer.
What companies has Tim Harding worked for?
Tim Harding has worked for Pfizer, Freelance, Thermo Fisher Scientific, Prometheus Biosciences, Inc., and Ignyta, Inc..
How can I contact Tim Harding?
You can use AeroLeads to view verified contact signals for Tim Harding at Pfizer, including work email, phone, and LinkedIn data when available.
What schools did Tim Harding attend?
Tim Harding holds M.S., Molecular Pathology from Uc San Diego School Of Medicine.
What skills is Tim Harding known for?
Tim Harding is listed with skills including Cell Biology, Drug Discovery, Biotechnology, Oncology, Clinical Development, Drug Development, Life Sciences, and Molecular Biology.
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