Tyler Gibson Email & Phone Number

Boston, Ma, Us

Boston, Ma, Us

- Head of Molecular Innovation Department within Research organization, comprised of Vectorology and Systems Biology functional teams- Led and managed department’s responsibilities to provide support across Research efforts ranging from early discovery and target identification to providing SME support for CMC of clinical-stage product.- Drove Decibel’s AAV vector technology platform strategy including further improvements of screening capabilities and the generation of additional late-stage preclinical gene therapy candidate vectors

Boston, Ma, Us

- Co-invented, with AAV process development collaborators, up to ten-fold improvements of AAV vector yield in upstream process.- Led cross-functional technology development team to generate novel molecular capabilities in the inner ear- Supervised Vectorology team's development of multiple AAV-based product candidates and establishment of complementary cell-based bioassays for each candidate.

Boston, Ma, Us

- Head of Vectorology function, responsible for design, sourcing, and analytics of all research AAV for all pipeline and platform programs- Hired Vectorology team members and supported their professional development- Supported hiring of new process development and analytical development teams and transfer of expertise to better enable AAV production, testing, and development- Championed and co-led transition of ELN to Benchling to leverage its molecular biology software capabilities to strengthen vector data tracking, leading to better ELN compliance across the Research organization- SME for multiple qualified and validated analytical assays to support GLP and toxicology preclinical studies for DB-OTO that directly supported the IND filing

Boston, Ma, Us

- Establish and led vectorology functional group to unify and centralize molecular biology expertise and lessons learned- Assisted in onboarding novel serotypes for discovery experiments- Brought crude AAV production in house for early discovery experiments

Boston, Ma, Us

- Organized and led Decibel's viral vector design and virus sourcing for discovery projects, including lentiviral, adenoviral, and AAV vectors.- Generated viral transgenes for prospective gene therapies across multiple programs.- Developed novel transgene regulatory elements to enable AAV-based gene therapies.- Demonstrated capabilities for targeted genetic perturbations in inner ear cells to facilitate early discovery research.

In Eaton Peabody Laboratory, I studied the transcriptional and epigenetic regulation of otic and auditory hair cell differentiation with Dr. Albert Edge. Using novel inner ear organoid culture, targeted epigenetic and transcriptional tools, and the development of new transgenic mouse lines, I probed the regulatory networks that drive hair cell generation.Podium presentation given:-Gibson, T.M., D. Sanders, and Albert S.B. Edge. "Locus-specific epigenetic modulation of transcription factor expression in differentiating inner-ear organoid culture." 41st Annual MidWinter Meeting of the Association for Research in Otolaryngology, February 2018.Abstract presented:Gibson, T.M., D. Sanders, M. Bradley, and A.S.B. Edge. “Targeted Transcriptional Activation and Repression of Hair Cell Reprogramming Factors.“ International Society for Stem Cell Research. June 2017. Poster Presented.

Boston, Ma, Us

Durham, North Carolina, Us

My work with Dr. Charles Gersbach focuses on understanding the processes that drive stem and primary cells to make fate decisions to differentiate into their ultimate lineage. This information is relevant to improving control over cellular phenotype for cell and gene therapies. I use a variety of genetic engineering techniques as well as direct single-molecule detection to analyze this decision-making process in individual cells. Platform presentations given:-Gibson, T.M. and C.A. Gersbach. “Single-Cell Analysis of Myogenic Differentiation and Reprogramming.” Annual meeting of the American Society for Gene and Cell Therapy, May 2014.-Gibson, T.M. and C.A. Gersbach. “Single-Cell Dynamics of Induced Lineage Commitment.” National meeting of the Biomedical Engineering Society, October 2012.-Gibson, T.M. and C.A. Gersbach. “Single-Cell Dynamics of Lineage Commitment.” The Society for Biological Engineering’s 3rd International Conference on Stem Cell Engineering. May 2012.-Gibson, T.M. and C.A. Gersbach. “Heterogeneity in induced lineage commitment: A Single-Cell Perspective.” National meeting of the Biomedical Engineering Society, October 2011.Abstracts presented:-Gibson, T.M. and C.A. Gersbach. “Single-Cell Analysis of Myogenic Differentiation Reveals a Bimodal Lineage Commitment System.” International Society for Stem Cell Research. June 2013. Poster Brief and Poster Presented.-Gibson, T.M. and C.A. Gersbach. “Single-Cell Dynamics of Skeletal Muscle Lineage Commitment.” North Carolina Tissue Engineering and Regenerative Medicine Society. September 2012. Poster Presented.

Durham, North Carolina, Us

Taught lab section for the course "Modeling Cellular and Molecular Systems"Department of Biomedical Engineering, BME 100/207 (re-numbered)Fall 2011, Fall 2012• Independently managed and taught lab section to undergraduates• Supervised a MATLAB-based project simulating cellular dynamics• Subjects covered: thermodynamics, enzyme kinetics, inducible cellular and genetic systems, mathematical modeling of cellular processes

Cambridge, Ma, Us

• Proposed and ran experiments to validate biological activity of primary cells in a micropatterned, microfluidic system.• Identified and demonstrated gene expression of targeted genes in primary cell line in a collaborative research team.

Norman, Oklahoma, Us

• Developed a Fortran program using genetic algorithms to optimize empirical thermodynamic data as a method to investigate predicted three-dimensional protein folding.• Applied generalized methods of quantifying the free energy change of solvation to simulate the effect of hydrophobic collapse in protein folding.• Investigated alternate optimization protocols and their computational efficiency when compared against genetic algorithms.

Spring, Texas, Us

• Managed production and certification of reference fuel products and corrected production errors. • Designed interactive programs to facilitate data tracking for the Fuels Blending department. • Planned capital improvements to plant infrastructure to improve production quality, worker safety, and reduce the environmental impact of fuel production. • Developed new means of automated communication between Fuels Blending engineers and the local Supply Team and Scheduling department.

Norman, Oklahoma, Us

• Isolated and decellularized the human umbilical vein for use in tissue engineering experiments.• Performed quantitative and qualitative analysis on the effects of decellularization on the adhesion of human gingival fibroblasts to the human umbilical vein.• Extracted primary cells from umbilical tissue and maintained live cell cultures.

Doctor Of Philosophy (Ph.D.), Biomedical Engineering

Master Of Science (M.S.), Biomedical Engineering

Bachelor Of Science (B.S.), Chemical Engineering