Triona Kirabo Email & Phone Number

United Kingdom

As a Laboratory Technician, I am responsible for the manufacture and purification ofproteins within the production team. I carry out the completion of quality and batch records.Performing quality control testing such as electrophoresis techniques e.g. ELISA and SDS,Lowry, optical density, RID, etc are crucial. Preparation and use of columns, buffers andreagents are essential in my role. Calibrating and operating key laboratory equipment whichcomplies with BBI’s quality system requirements are also vital. Completing daily cleaningchecks and ensuring I maintain my individual training records is fundamental. Whilstadhering to all SOPs, PSBRs, EOPs, SMPs and prioritising meeting any daily productiontargets.

Uganda

I was honoured to work in a BSL3 laboratory under GMP conditions, as a research internunder the MRC/UVRI and LSHTM Research Unit in Uganda. The research project wasassociated with local hospitals where our samples were collected via post mortem study.Immunity against M.tb is localised at the site of infection, the lungs. Lung draining lymphnodes and bone marrow have also been shown to contain TB specific memory cells. TheTBPM study aimed to compare lung immune cells in active TB, against those in latent TBand those uninfected individuals using flow cytometry. However, analysis by flow cytometryis only possible for single cell suspensions, whereas cells in tissues only occur in an extracellular matrix. Therefore, these cells must first be released from the extra cellular matrix intoa single cell suspension. In order to isolate individual cells from the lung tissue, the tissueswere dissociated enzymatically and physically to liberate the cells from the extra cellular matrix. Enzymatic dissociation was achieved using a digestion mixture which containsCollagenase D and DNase I which digest extra cellular matrix and DNA respectively. Themixture was physically dissociated using a GentleMacs and passed through a sieve to obtaina single cell suspension. The suspension is purified to remove RBCs and non-mononuclearcells. PBMCs in the TBPM study were isolated from blood samples by density centrifugationusing lymphoprep as a cell separation medium for recovery of mononuclear cells. IsolatedPBMCs were used in subsequent analysis for cell surface staining, intracellular staining, etc.The remaining cells will be used for further studies.

United Kingdom

A QC Analyst’s role is mainly shaped around testing raw and incoming materials plusfinished products according to the appropriate specifications, test methods and GMPconditions. The usage of HPLC, Refractometer, Auto-titrators, pH meter, osmometer etc areall pivotal parts of this role. At CooperVision I visually inspect the media filled for microbialgrowth whilst primarily performing in process checks on bulk products both pre and postfiltration whenever necessary. I ensure that all laboratory equipment for example theOsmometer, Viscometer, Auto-titrators, etc is routinely calibrated and is in good workingorder alongside recording all results as per cGMP standards. I also prepare and maintain arange of standard solutions and bench reagents for example Manoxol, Diphenyl-carbazide,Molybdovanadic reagent, etc, used in the quantitative and qualitative analysis of incomingsamples, within the cGLP framework. Furthermore, meeting statutory, regulatory andcustomer requirements, within GCLP and AQC framework, is one of our main objectives as acompany.

Master'S Degree, Forensic And Medical Sciences

Doctor Of Philosophy - Phd, Chemistry And Pharmacy

Doctor Of Philosophy - Phd, Chemistry And Pharmacy

Ongoing

Doctor Of Philosophy - Phd, Medicinal And Pharmaceutical Chemistry

Ongoing