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Hello! I’m Trent, a proud San Diego native and Senior Research Associate specializing in enzyme engineering and high-throughput cloning. With a Bachelor of Science in Biology focused on Cellular and Molecular Biology from San Diego State University, I bring a solid foundation in molecular techniques and a passion for innovation in biochemistry.In my current role at Geno, I enhance project pathway enzymes by designing mutagenesis libraries utilizing protein modeling software, historical data, and literature. I develop enzyme assays to identify improved features and leverage automated robots to efficiently set up and screen thousands of enzyme variants. I then purify and characterize promising hits, which I present to the strain team for integration. In addition, I conduct in vitro assays on fermentation and quantitative small scale pellets to understand how these enzyme variants perform in an integrated context. Previously, I honed my skills in plasmid construction and cloning, managing hundreds of plasmid requests and evolving our plasmid inventory for automation compatibility. My early experiences as a lab assistant and undergraduate researcher provided me with a comprehensive understanding of laboratory operations and experimental design.I am passionate about contributing to groundbreaking research that pushes the boundaries of enzyme applications and biotechnology. I thrive in collaborative environments and am always eager to embrace new challenges in the field.
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Senior Research AssociateCrop DiagnostixSan Diego, Ca, Us -
Senior Research Associate I - Enzyme EngineeringGeno Oct 2022 - Oct 2024San Diego, California, United StatesCreated mutagenic library designs for several enzymes, utilizing protein modeling software (Discovery Studio) and historical data to pinpoint mutational spaces that will improve enzyme features. Took charge in developing and optimizing multiple enzyme assays and workflows, enhancing the characterization and assessment of target enzymes. Leveraged automated robots (Echo, Bravo, Qpix) to efficiently set up and screen thousands of enzyme variants. Meticulously processed, analyzed, and ranked their performance utilizing tools like Gen5, Excel, and Spotfire applications. Presented data and results on campaign screens, offering insights for optimizing enzyme hits and providing recommendations for strain integration.Conducted weekly in vitro bench-top assays on fermentation time-course pellets, analyzing enzyme expression and activity, and communicating findings and next-steps to the team. -
Research Associate I/Ii - High Throughput CloningGeno Mar 2019 - Sep 2022Designed and constructed thousands of single and multi-gene plasmids using golden-gate cloning, gibson assembly, and other PCR techniques. Leveraged automated equipment (Echo 525 and Qpix) to simultaneously construct hundreds of plasmids. Concurrently, kept plasmid requesters well-informed about the progress of their plasmid requests.Evolved the current plasmid inventory (>1000 plasmids) to be automation compatible which included meticulously recording plasmid concentration, final plasmid destination, and glycerol stock locations.Developed and created plasmid-based tools to streamline CRISPR Cas9/Cas12a editing workflows, including constructing CRISPR arrays, plasmid-based homology arms, and guide plasmids. -
Lab Assistant - OperationsGeno Feb 2018 - Mar 2019Contributed to the seamless execution of research activities by ensuring the availability and quality of crucial resources for experiments (media plates, buffers, antibiotics, chemical or electro-competent cells, and other media requests). Successfully conducted hundreds of plasmid extractions on a daily basis in an efficient and quality matter to streamline researcher's workflows. Took charge of coordinating the pick-up and delivery of express mail services, managing inventory logistics and stocking essential supplies to ensure uninterrupted research operations. Meticulously labeled, stored, and maintained accurate records for incoming chemicals, prioritizing safety and compliance.Diligently carried out daily washing and autoclaving of glassware and supplies. Maintained and monitored -80°C freezers and coordinated calibration services for lab equipment. Established an organized, clean, and safe lab space. -
Undergraduate ResearchSan Diego State University Aug 2017 - Feb 2018Utilized C1-Biocatalysis and dry fermentation techniques to sustain active methanotrophic cells on a specialized filter for a bio-filtration device targeting methane reduction. Managed a bioreactor fermentation system, analyzed and presented research findings, prepared media, and ensured a sterile lab environment.
Trent Smith Skills
Trent Smith Education Details
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Cell/Cellular And Molecular Biology
Frequently Asked Questions about Trent Smith
What company does Trent Smith work for?
Trent Smith works for Crop Diagnostix
What is Trent Smith's role at the current company?
Trent Smith's current role is Senior Research Associate.
What is Trent Smith's email address?
Trent Smith's email address is ts****@****ica.com
What schools did Trent Smith attend?
Trent Smith attended San Diego State University.
What skills is Trent Smith known for?
Trent Smith has skills like Microsoft Office, Customer Service, Microsoft Excel, Microsoft Word, Microsoft Powerpoint, Public Speaking, Research, Time Management, Polymerase Chain Reaction, Microscopy, Cell Culture, Protocol Buffers.
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Trent Smith
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Trent Smith
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