Group of Stamatis PapathanasiouMainz, RP, Germany

• More than 20 articles posted on The Investigator Taiwan.• Creating more than 45K views on WordPress.

• From Structural biology, Microbiology, Metabolism, to Cancer biology, bringing cutting-edge life science knowledge to first-line scientists.

• First author publication: Multiomics reveal the central role of pentose phosphate pathway in resident thymic macrophages to cope with efferocytosis-associated stress. Cell Reports · Jul 12, 2022Thymic-resident macrophages, a rare population that only represents 0.1% of total cells in the thymus organ, play an essential role in removing apoptotic thymocytes and maintaining thymus homeostasis. However, it remains unclear how thymic-resident macrophages can efficiently engulf… Show more • First author publication: Multiomics reveal the central role of pentose phosphate pathway in resident thymic macrophages to cope with efferocytosis-associated stress. Cell Reports · Jul 12, 2022Thymic-resident macrophages, a rare population that only represents 0.1% of total cells in the thymus organ, play an essential role in removing apoptotic thymocytes and maintaining thymus homeostasis. However, it remains unclear how thymic-resident macrophages can efficiently engulf apoptotic thymocytes. Therefore, we sorted the primary macrophages and performed metabolomics, RNA sequencing, and single-cell RNA sequencing analyses. Together with the in vitro studies, our work demonstrates the thymic-resident macrophages overcome continuous efferocytosis-induced oxidative stress by up-regulating the PPP, which highlights the central role of the PPP in maintaining thymic-resident macrophage and efferocytotic macrophage functions.During the research, I formed the hypothesis of PPP supporting the neutralization of oxidative stress in the thymic macrophages. With Dr. Chia-Lin Hsu’s guidance, we designed the proof-of-concept experiments, including the breakthrough of the challenging ex vivo thymic explant treatment. Furthermore, to achieve the complex multi-omics analysis of the metabolism network, I had learned R programming to accomplish the overall understanding of thymic macrophage metabolism. Together with flow cytometry, primary cell sorting, immunofluorescence staining, mitochondria content measurement, and cell culture experiments, we have put all the pieces together to prove our hypothesis. Not only did I learn bioinformatics skills and perform experiments, but I had also built a complete study from scratch. After three years of hard work in the laboratory, we have set up a milestone in investigating the rare thymic population. Show less

• Dean's Award of College of Life Science, National Yang-Ming University• Excellent Master Thesis Award, National Yang-Ming University• Honor member of Phi Tau Phi Honor Society• Dr. Hsu, Chialin's lab• Dissertation: https://hdl.handle.net/11296/7my26cTaipei, Taiwan

• CGU_Taiwan, The International Genetically Engineered Machine Competition• Rewarded for the Bronze medal of the International Genetically Engineered Competition• Team leader of CGU_Taiwan (Chang Gung University)• Project: INKOFF: Preserve Fibers to Save NatureReprocessing paper is very important for reducing deforestation, but the current manufacturing process causes chemical pollution and substantial paper fiber losses. After extensive discussions with paper-making… Show more • CGU_Taiwan, The International Genetically Engineered Machine Competition• Rewarded for the Bronze medal of the International Genetically Engineered Competition• Team leader of CGU_Taiwan (Chang Gung University)• Project: INKOFF: Preserve Fibers to Save NatureReprocessing paper is very important for reducing deforestation, but the current manufacturing process causes chemical pollution and substantial paper fiber losses. After extensive discussions with paper-making experts, we have devised a plan to replace the chemicals used in traditional paper reprocessing and to increase recovery of paper fibers. In our model, the paper sheets undergo image analysis to determine which spots have ink printed on top and will be sprayed with Saccharomyces cerevisiae which has been engineered to express deinking enzymes upon light induction. Instead of treating all paper pulp, only the spots with ink is shone with light to induce localized deinking. This procedure can remove ink efficiently while protecting most of the paper fibers from being digested. This design extends the lifetime of the paper fibers and reduces wood-logging for making paper. Show less

• Protein expression of Thymic stromal lymphopoietin in OVA-sensitized mice model• 8 months• Professor Ming-lin Kou's lab• We are working on finding the potential target for AAV knockdown in asthma therapy by using OVA-sensitized mice model.

• Development of a protease-activated antibody that reduces the side effects of Rheumatoid Arthritis therapy• 2 months (summer project)• Professor Tian-Lu Cheng's lab, Kaohsiung Medical University• We tried to lower the side effects of Remicade, Humira, and other mAb drugs by adding a protease-activable blockage on the epitopes.

• The cell cycle and the cell segregation• 6 months• Professor Scott C. Schuyler's lab• We study the interaction of CDC20 and APC in cell cycle by using Saccharomyces cerevisiae.