Shamini Ramkumar Thirumalasetty Email & Phone Number

Dresden, Sn, De

Germany

Dresden, Saxony, Germany

Novel therapeutic approaches for Congenital Adrenal Hyperplasia (CAH)Congenital Adrenal Hyperplasia (CAH) is a genetic disorder affecting the adrenal glands' ability to produce vital hormones like cortisol and aldosterone. This condition leads to an imbalance of hormones, resulting in various symptoms such as abnormal development of sexual characteristics and potential adrenal crisis. Treatment typically involves hormone replacement therapy to manage symptoms and ensure normal growth and development.Skills: Literature research & funneling, mammalian cell culture, mouse line characterization (In vitro), RNA isolation, cDNA preparation, qPCR analysis, sequencing, PCR, staining, histology & imaging,cloning and transfection, SDS-PAGE and immunodetection, Immunostaining & imaging, Bulk-RNA sequencing, Manuscript preparationProgramming & Softwares: R Programming, Python, GraphPad prism, ImageJ, PyRAT, Quantstudio, Snapgene, Mutation surveyor, LaTeXPublications:DOI: 10.1210/jendso/bvac062 (2022)DOI: 10.3390/ijms25105062 (2024)

Dresden, Saxony, Germany

Elected representative of TU Dresden's official council for over 6000 doctoral studentsTasks of the doctoral council:- Administration of finances- External communication and marketing (e-mail, website, social media, VMPV)- Assembly and meeting organisation- Secretarial work / documentation- Event management (DDocs cafe, Science Beer, Junior Scientist Party)Official website: https://tu-dresden.de/Doctral councilMembers: https://tu-dresden.de/9th doctoral council

Board member - Young Active Research in Endocrinology (YARE); Young scientist wing of Deutsche Gesellschaft für Endokrinologie (DGE)Organizing committee- YARE meeting 2024 in Dresden, GermanyFind more details in https://www.yare-endo.de/

Hamburg, Germany

Core area of work: Application product supportProvided assistance in managing the Application Knowledge Database set up for managing Eppendorf equipment used by customers worldwideSkills acquired:- Product knowledge (Centrifuges, Deep freezers)- FAQ preparation - Analyzing customer problems/queries- Customer support

Hamburg, Germany

Project title: "Enzymatic Degradation of Polyethylene Terephthalate (PET): Characterization of Novel Hydrolases from Proteobacteria and Validation of an Oxidative Approach“Grade: 1.0- Expression and characterization of PET hydrolyzing enzymes from Proteobacteria - Oxidative approaches towards PET degradation and treatmentSkills acquired: - Strain maintenance- Bacterial growth and cultivation- Monitoring bacterial growth cultivation- Cell harvesting- PCR- Gene expression studies-Preparation of crude cell extracts- Protein purification- Dialysis - Protein quantification- SDS-PAGE, Western blot- Plate assays: Para-nitrophenol (pNP) ester assay- Activity assays- Degradation assays: P450 Peroxygenase (CYP 152A1)- HPLC- Sequential and structural comparisonPrograms & Databases: MEGAX, T-coffee, Robetta, BLAST, USCF Chimera, UniProt, RCSB PDB, JGI IMG

Leverkusen, North Rhine-Westphalia

Core area of work: Biotechnology, E&T- Worked in a family development project with difficult to access micro-organism. - Carrying out molecular and microbiological work such as cloning, transformations and strain management. - Genomic integration of vector constructs for over expression in the target organism.- Characterization of the developed strain and subsequent enzymatic conversions. - Carrying out, evaluating and presenting the results database and literature research.Skills acquired:- Working in S2- Cloning- Transformation- Strain management- Genomic integration- Vector construct manipulation- Overexpression techniques- Strain characterization- Enzymatic conversion analysis- Literature research- Result evaluation- Data presentation skills

Hamburg Area, Germany

Core Area of work: Developmental Microbiology, R&D- Conducting volunteer studies to study the skin microbiome and its implications in the in vivo skin model and in vitro skin model.- Testing various substances used in formulations to analyse their effect on the skin microbome. - Helping in optimising the concentration of the substances used in formulations by conducting various experiments.- Conducting experiments to draw a comparison between in-house and out-house products.Skills acquired: - Volunteer studies- Skin microbiome analysis- In vivo skin model testing- In vitro skin model testing- Formulation testing- Substance effect analysis- Concentration optimization- Experimental design- Comparative analysis- Product evaluation

Hamburg, Germany

Project: "CRISPR/Cas9 facilitated genome engineering of E-Coli for the efficient biosynthesis of L-tryptophan "- Engineered the E.coli strain to optimize the production of L-Tryptophan. - Genetic and metabolic changes were implicated in the biosynthetic pathway which were finally verified by fermentation. - Techniques utilized for the study include : CRISPR/Cas9 for genome editing, Infusion Cloning, Colony PCR, DNA extraction, Plasmid isolation, Fermentation and plasmid construction using Snapgene.Skills acquired:- Genome editing (CRISPR/Cas9)- Cloning (Infusion Cloning)- Polymerase chain reaction (Colony PCR)- DNA extraction- Plasmid isolation- Fermentation- Molecular biology techniques- Biosynthetic pathway analysis- Strain optimization

Hamburg Area, Germany

Project: Degradation of Nylon using Extremophiles- I largely focused on preparing enrichment cultures and incorporated various plating techniques for the growth and optimization of the microorganism of interest.- I also conducted studies to check the growth of the microbe and the degradation of nylon over a certain period of time. For this, I have used techniques such as DNA extraction and PCR techniques such as 16s PCR and colony PCR.Skills acquired: - Enrichment culture preparation- Microorganism growth optimization- Plating techniques- Microbial growth studies- Nylon degradation studies- DNA extraction- Polymerase chain reaction (PCR)- 16s PCR- Colony PCR

Bengaluru, Karnataka, India

Core Area of work: Bioprocess Engineering & Mammalian Cell Culture Technology- Conducted Reactor studies to evaluate the behavior of CHO cells in different commercial media. - Additionally, I had run multiple trials to optimize the growth of CHO cells and study their kinetics.- Upon the success of the trials, I ran the same batch and tried to optimize their growth in a reactor and conducted further evaluation studies.Skills acquired:-Reactor studies -Kinetics analysis-Bioprocessing evaluation-Experimental design-Data analysis

Bengaluru, Karnataka, India

Core Area of Work: Process Analytics in Mammalian Cell Culture - Handled the Amino Acid and Immunoglobulin analysis for 2 novel drugs and 4 biosimilars along with their validation, evaluation, and documentation. - Gained hands-on experience with Agilent’s High-Performance Liquid Chromatography (HPLC) and Ultra High-Performance Liquid Chromatography (UHPLC) systems for analysis.- These analyses played instrumental in obtaining the titer of the ongoing batches ranging from the lab scale to the production scale which was thereby helpful in determining the productivity of their ongoing bathes along with helping us identify the itinerary.- The amino acid analysis done using the UHPLC helps us identify the amino acids present in a specific media processed within a Bioreactor and contributes towards feed and growth studies.Skills acquired- Amino acid analysis- Immunoglobulin analysis-Validation & documentation-High-Performance liquid chromatography (HPLC)-Ultra high-performance liquid chromatography (UHPLC)-Batch titer determination-Productivity assessment-Bioreactor feed & growth studies

Bangalore Urban, Karnataka, India

Project: " Expression and Purification of SIRT1 and SIRT2 of the human sirtuin family of proteins"Thesis grade: 99%Top of the graduating class of 2016 in the Department of BiotechnologyI worked as a undergraduate trainee under the guidance of Dr. Padmanabhan Balasundharam ( Senior Professor, Department of Biophysics, NIMHANS).- I worked on various molecular and structural biology techniques and also on a few aspects of protein engineering. - This project included hands-on training and a thesis submission. - During the project, I carried out various molecular biology and chromatography techniques which included studying the relevance of these active homogeneous proteins to behave as therapeutic targets to treat neurodegenerative diseases such as Alzheimer's, Parkinson's and Huntington's.Skills acquired:- Cloning- Tranfection, transformation- Protein extraction- Protein purification using ÄKTA systems- Western blotting

Greater Bengaluru Area

Interned at the pharmaceutical unit and gained an understanding about the general functioning of different manufactural processes. I have also acquired the know-how of the preparation of various generic as well as branded drugs, their testing and purification.Gained experience in:- Workstation plan- Handling Quality Assurance documentations - Hands on experience in handling quality control instruments

Doctor Of Philosophy - Phd, Molecular Endocrinology

Thesis: Identifying Novel Therapeutic Targets for Congenital Adrenal Hyperplasia (CAH) CAH is a genetic disorder affecting the adrenal.

Master Of Science - Ms, Chemical & Bioprocess Engineering, 1.8

Activities and Societies: Editor - " SANGAM" - Intra University Monthly magazineGraduated top 5% of my class Core Subjects : Bioprocess.

Bachelor Of Engineering - Be, Biotechnology, 90%

Activities and Societies: -Head student Coordinator for a workshop and Biotechnological fest, Student Placement Coordinator.

High School Diploma

Activities and Societies: Member of the philanthropy club, Basketball , Throwball.