• Lead method transfer, qualification, and validation.• Support stability, in-process and release testing for allogeneic cell therapy programs.• Manage critical reagent inventory.• Lead EHS and cross-functional alignment initiatives.• Learn and promote quality mindset and culture.• Use of Trackwise, BMRAM, and Vaisala for compliance.

• Worked with departmental and cross functional peers to execute on assignments, under limited supervision.• Run RNA extractions using KingFisher Flex system and physical viral titer assay by ddPCR.• Maintained HEK293T, Jurkat, NALM6 cell lines.• Developed and optimized Flow (Agilent NovoCyte, BD FACSCanto) based infectious titer assays.• Qualified IL-2/IL-15 ELISA (MSD, ELLA) for a potency assay transfer.• Solved technical issues for a release testing method and provided training.

• Support viral vector lab, pilot plant, upstream and downstream characterization and development programs by testing process related impurities at Takeda Pharmaceuticals.• Run RNA extractions using KingFisher Flex system and physical viral titer assay by ddPCR. • Maintain HEK293T and Jurkat cell lines.• Perform testing of CHO-HCP, and PLBL2 ELISAs on various platforms (Cygnus, Gyrolab, TECAN).• Automate standard analysis methods with Liquid Handling Systems such as JANUS, and TECAN in collaboration with analytical development.• Quantify sample purity by CE-SDS (LabChip GXII). Determine protein concentration using Lunatic quantification system.• Maintain cross-training efforts on HPLC and icIEF methods.• Identify technical needs and design/perform experiments/projects to contribute process improvement efforts.• Document accurate and high-quality data using electronic notebook with >%95 deadline target match.• Communicate and present data internally within in process analytics team as well as cross functionally to better serve team goals and company values.• Present “Overcoming limitations of ELISAs in process-related impurity testing” webinar in Cambridge Healthtech Institute.

• Coordinated technology transfer of animal vaccine products to a manufacturing organization considering European Union cGMP regulations.• Monitored production of Porcine circovirus and Mycoplasma Hyopneumoniae using in process assays in coordination with manufacturing, and QA/QC departments.• Completed three pre-qualification reports for EU regulators within the first five months of employment. • Maintained cell cultures and stocks. • Documented and maintained laboratory records.• Prepared standard solutions according to prescribed formula.• Prepared and execute new standard operating procedures and study protocols.• Successfully performed color changing, plaque/colony forming, TCID50, bicinchoninic acid, immunofluorescent, and thiosulphates assays. • Run western blot, indirect and sandwich ELISAs. • Completed training on GE Xcellerex XDR bioreactor.

• Led thesis project titled: “From single chemicals to complex mixtures” effect of contaminants of emerging concern on behavior and gene expression of fathead minnows across three life stages• Executed RNA extractions, qPCR, vitellogenin ELISA, predator escape performance, feeding efficiency, and behavioral assays in fathead minnows.• Carried out tasks such as chemical exchange, flow calibration, feeding, water quality control, spawning, YSI and temperature measurements, as well as dissection, and embedding, sectioning, staining tissue samples as part of the 300+ days of in vivo exposure study• Analyzed, and organize data using statistical software • Investigated exposure related contamination and micropipette calibration issues and participate in the quality assurance committee to propose an action plan on improving good laboratory practices• Presented my thesis research at following professional conferences: o Midwest Fish and Wildlife Conference 2018 o Wastewater Operations Conference, Minnesota Pollution Control Agency 2018 o Young Environmental Scientists Conference, SETAC 2018 o Minnesota Water Resources Conference, University of Minnesota 2017 o Society of Environmental Toxicology and Chemistry North America 2017 & 2018 o Annual SCSU Student Research Colloquium 2016, 2017 & 2018

• Taught laboratory portion of Human Anatomy & Physiology, and Microbiology courses.• Established course syllabus, material and schedule for Research in Aquatic Toxicology.

• Applied interactive learning methods to increase student understanding of the course materials.

• Enhanced engagement of students in Cell Function & Inheritance and Classical Physics laboratories • Managed laboratory notes, review and process the lab data with students

• Reinforced multiple studies on exposure of the prevalent MN fishes during various life stages to contaminants of emerging concerns to understand mechanisms of toxicity and the responses to toxic agents in aquatic environments while gaining substantial problem solving and effective task prioritization experience.

• Achieved exposure objective on the effects of Bisphenol-A, on type-1 diabetes development in NOD and C57Bl/6J mouse models• Adhered to the IACUC policies along with laboratory SOPs and guidelines while performing glucose and weight measurements, drug preparation, intraperitoneal injections and routine inspection of mice health.• Obtained $1,000.00 award of student research funds for the project entitled “Effects of Exposure to Combined Persistent Organic Pollutants (POPs) on Development of Autoimmune Diabetes in NOD Mouse Model”.• Accelerated partnership with Aquatic Toxicology Lab on isolating neutrophils from kidneys of fathead minnows treated with complex urban mixture to understand functional changes in immune system.• Presented a research poster titled “BPA increases incidence of Type 1 Diabetes in C57Bl/6J Mouse Model” at the Annual SCSU Student Research Colloquium 2016.

• Established a diverse and socially just residential community based on respect, safety, responsibility, trust and care.• Demonstrated role model behavior while developing student directed community advisor supported approach.

• Led the RHA Programming Committee and its $9500 yearly budget while introducing implementation of transformational leadership to the committee which consists of 3 board members, and 10 volunteers.• Organized and promoted 15 supportive, informative and elated events throughout the year for over 3000 hall students.