Learned about the technical knowledge about Biological Databases and its applications, Alignment of Multiple Sequence using online tools Clustal-Omega, MAFFT, MUSCLE, Jalview, MEGA-11RNA extraction and quality checking and concept of qPCR, setting up of Real-Time PCR and its analysis, Protein structure visualization and prediction using Protein Database, Validation of Protein Structure and 3D visualization using PyMOL SoftwareLearned the technique of Artificial Insemination, Semen Cryopreservation and In-vitro Fertilization, Literature Searching, Primer Designing (from exon and intron) and Validation of primers by In-silico tools, RNA Extraction from the tissues, Setting up PCR and qPCR, Gel ElectrophoresisPrepared the buffers for SDS PAGE, SDS PAGE (hands-on), Prepared the media for Cell culture and sterilization, Cultured Animal Cells, Prepared broth and agar media for bacterial culture, streaked bacterial culture on agar medium, Stained and identified bacterial cells