Vamsi Krishna Bolla Email & Phone Number

Galloway, Oh, Us

Rockville, Maryland, United States

Provide expert guidance and technical support in research projects, specializing in bioprocessing and downstream purification techniques. Manage and maintain advanced bioreactor systems, overseeing cell culture operations and ensuring the consistent quality of experimental outcomes. Collaborate with faculty and research teams to design experiments, analyze data, and prepare findings for publication and presentations. Mentor and train junior researchers and students in laboratory techniques and safety protocols.● Developed and optimized laboratory protocols, ensuring adherence to best practices and research objectives.● Successfully executed protein purification protocols, contributing to the smooth operation of the laboratory.● Maintained and managed cell bank inventory, ensuring the availability of critical resources for ongoing research projects.● Demonstrated proficiency in using PRISM and Benchling for data analysis, enhancing the accuracy and clarity of research findings.● Operated and maintained bioreactors, including the Ambr 250 high throughput system, to support upstream processing tasks.● Applied expertise in molecular biology techniques, including Octet assays, to advance project objectives and deliver precise analytical results.

Maryland, United States

Maintained cell bank inventory and perform routine cell culture to support research initiatives. Operate 2L single-use Eppendorf bioreactors for bioprocessing applications. Execute protein purification processes, including affinity chromatography (ProA), ion exchange, and size exclusion chromatography (SEC). Prepare feeds and buffers essential for cell culture and purification procedures.

Banglore

Managed bioreactor operations, including Ambr250 high throughput systems and 5L benchtop glass bioreactors, ensuring efficient batch creation, processing, and harvesting. Conducted cell bank management, including vial thawing for MCB, WCB, and DCB, and performed clone screening to establish stable cell lines. Oversaw media screening and optimization, contributing to the development of improved media, feeds, solutions, and buffers. Addressed operational issues in bioreactors through effective troubleshooting. Handled multiple client projects, demonstrating proficiency in change control, deviations, CAPA, and SOP establishment. Maintained project and inventory management to support process development initiatives.● Optimized bioprocessing operations, resulting in enhanced stability and productivity of established cell lines.● Successfully managed the scale-up process from 5L to 80L, employing depth filtration and clarification techniques.● Streamlined project workflows, leading to more efficient management of client projects and inventory.● Improved media and feed strategies, contributing to the advancement of process development.● Implemented and updated SOPs, ensuring compliance and consistency across biopharmaceutical operations.

Bengaluru, Karnataka, India

Cultured cells and prepared media and reagents for various experiments. Operated a 10L Sartorius fermenter for bioprocessing applications. Conducted PCR, designed primers, and performed Agarose gel electrophoresis for DNA analysis. Executed plasmid cloning and extracted plasmid DNA. Transfected cells with plasmids to study gene expression.● Effectively managed bioprocessing tasks using a 10L Sartorius fermenter, contributing to the lab's experimental success.● Applied molecular biology techniques, including Bacterial Transformation and DNA extraction, to support research objectives.● Demonstrated technical proficiency in primer design and PCR, leading to accurate DNA analysis and research insights.

Jedcherla

Prepared and optimized buffers for downstream processing in R&D, focusing on the purification of red blood cells stimulating factors. Executed purification protocols using industry-standard GE Akta Avant, Akta Purifier, and Akta Prime systems. Collaborated on project management tasks, ensuring streamlined operations and adherence to research timelines.● Successfully executed purification protocols using GE Akta systems, contributing to the advancement of red blood cells stimulating factors research.● Demonstrated technical expertise in ultrafiltration and diafiltration processes, optimizing product concentration and purification.● Enhanced research efficiency through effective preparation and management of critical R&D buffers.● Contributed to project management, ensuring successful and timely completion of research objectives.● Gained valuable hands-on experience in automated and manual purification systems, strengthening skills in downstream bioprocessing.

Master'S In Professional Studies, Biotechnology

Bachelor'S Degree, Biotechnology

Intermediate, Biology, General