Toxicologist at the Center for Drug Evaluation and Research, OND, DPT-OCHEN at FDA

Center for Cancer Research, National Cancer Institute (CCR, NCI).Laboratory of Cancer Biology and Genetics (LCBG). • Established the guidelines and provided the resources and training for the isolation, characterization and evaluation of biological processes involving extracellular vesicles (EVs) for 11 research groups at NCI.• Established the use of extracellular vesicles (EVs) as novel biomarkers for ovarian and breast cancer in human patients and several murine cancer models.• Discovered anti-metastatic role of Chloride Intracellular Channel 4 (CLIC4) by inhibiting the in vivo spread of breast cancer metastasis with therapeutic potential. • Awarded with multiple honors by NIH and the International Society of Extracellular Vesicles. Recognized as NCI-wide expert in state-of-the-art techniques including biological isolation, purification, and related experimental and analytical techniques in EVs. • Sustained the budget and the outstanding performance ratings of the laboratory through two consecutive site visit reviews by external committees by establishing unprecedented results in the field. • A widely sought advisor, and mentor to six more junior scientists, including post-doctoral fellows.

Laboratory of Cancer Biology and Genetics (LCBG). • Pioneered EV research at the LCBG and led the development of EV expertise within the NCI using in vitro and in vivo cancer models.• Established collaborations with several NCI investigators leading to peer-reviewed publications. • Identified CLIC4 as circulating tumor biomarker in EVs for ovarian cancer using a xenograft model in combination with in vitro techniques.• Explored the effect of nitric oxide donors in the biology of CLIC4 as possible therapy to reduce tumor burden in vivo using skin xenograft mouse models and in vitro techniques.• Established the use of bone-marrow derived macrophages from wildtype and CLIC4 knock out mice to investigate the role of CLIC4 on macrophage activation and polarization. • Formalized Material Transfer Agreements (MTA) with national and international investigators regarding the sharing of CLIC4 floxed and knock out mice.• Acquired new technologies as project officer to perform EV-based research by applying and competing for HHS/NIH intramural funding.• Enabled, trained, mentored, and fostered postbaccalaureate fellows and summer students in research techniques, protocols, projects, and career development.

Laboratory of Cellular and Molecular Biology (LCMB)• Discovered a potential isoform of Granulysin, besides the well-known 15 and 9 kDa, with the capacity to induce cell death upon release by evaluation of its intracellular protein processing and secretion in natural killer cells. • Generated over 60 transgenic natural killer cell lines expressing different single or combined set of mutations for Granulysin by lentiviral transduction. • Managed and supervised the generation and maintenance of several murine animal colonies by working with animal husbandry personnel and core facility investigators.

Department of Pathology and Laboratory Medicine• Formulated the potential of carbon nanosheets to induce granuloma formation upon interaction with primary macrophages using a 3D in vitro culture system. • Enforced and provided guidelines and assistance to other laboratories about the safety and handling of nanomaterials.• Active participant on interdisciplinary projects leading to several co-author publications. • Authored peer reviewed publications and presented in scientific meetings.• Directed, supervised, and mentored laboratory personnel in research techniques, protocols, and projects.

• Engineered a 3D in vitro model of epithelioid granulomas induced by high aspect ratio nanomaterials able to predict in vivo outcome upon inhalation exposure.• Formulated assays to test in-house-made carbon composite nanoparticles for drug delivery. • Authored and co-authored peer reviewed publications and presented in national and international scientific meetings. • Trained laboratory personnel including students, technicians, and advanced degree holders in research techniques and protocols.

Brown University. Course: Molecular Mechanisms of the Disease. Professor: Carmen Marsit, PhD.

Division of Endocrinology. Department of Medicine. • Designed, conducted, performed, and optimized in vivo studies in mice and rats together with laboratory research experiments to evaluate the intracellular processing of Thyrotropin-releasing hormone (TRH) and its regulation by other hypothalamic hormones implicated in energy balance and thermoregulation.• Authored and co-authored peer reviewed publications and presented in scientific meetings.• Guided and oversaw a total of 7 laboratory personnel including students, technicians, and advanced degree holders in research techniques and protocols.

Research Group of Evolutive Biology (GIBE). Facultad de Ciencias Exactas y Naturales, University of Buenos Aires. Subject: Genetic polymorphism of the genus Culex in the city of Buenos Aires. Mentor: Esteban Hasson, Ph.D. - Breed and morphological characterization of mosquitoes.- Isolation and sequencing of specific mitochondrial genes.- Statistical analysis.

Research Group of Mosquitoes Population. Unit of Ecology and Evolution of Parasites and Vectors. Division of Parasitology. • Supervised research team of 3 members to evaluate the dynamics of water bodies at the Ecological Reserve of Buenos Aires as natural hatcheries of mosquitoes leading to the identification of 33 mosquito species, some of them first described at the area.• Identified and chronicled mosquito species and other arthropods by phenotypic characteristics from samples collected in the field, collected data, interpreted results to elaborate reports for University, advice Government authorities, and scientific presentations.

Research Center of Porphyries and Porphyrins (CIPYP). Facultad de Ciencias Exactas y Naturales, University of Buenos Aires. Argetnina. Subject: Regulation of heme oxygenase – 1 (HO-1) in a chemically induced hepatocarcinogenesis murine model. Mentor: Elba Vazquez, Ph.D. - Induction and follow up of hepatic carcinogenesis in murine models.- Evaluation of enzymes involved in the synthesis of heme group and oxidative stress-induced enzymes (enzymatic activity, Western blot, Northern blot and PCR).