Vanessa Zancanella Email & Phone Number

Oegstgeest, Zh, Nl

Amsterdam, Paesi Bassi

Amsterdam, Paesi Bassi

Amsterdam, Paesi Bassi

Gene therapy is a technique for correcting defective or missing genes that cause disease.Gene therapy offers the prospect of long-term and potentially curative benefit to patients with genetic or acquired diseases by directing the expression of a therapeutic protein or restoring the expression of a missing protein through a single administration.uniQure develops its gene therapies using an innovative and modular technology platform, including our proprietary, cost effective manufacturing process. Our gene therapy technology consists of three key elements:1. Therapeutic gene cassettes that carry a transgene that encodes, or provides the blueprint for the expression of, a therapeutic protein;2. An AAV-based vector delivery system for delivering the gene cassette;3. Administration technologies to effectively deliver the relevant transgene into the tissues and organs.

- Elucidate the molecular mechanisms involved in cytochrome P450 3A (CYP3A) expression and regulation in cattle liver- Absolute quantification of CYP3A28, CYP3A38, CYP3A48 transcripts in bovine liver

Faculty Of Veterinary Medicine

- Molecular biology: nucleic acid (RNA/DNA) isolation with phenol/chloroform method and commercial kits from different matrices; quantity (Nanodrop, QUBIT assays) and quality (electrophoresis) isolated nucleic acid evaluation; purification of nucleic acids, design of assays/primers gene- and/or isoform-specific for end-point and qPCR; Sanger sequencing; immunoblotting; microsomal/cytosolic fraction isolation form liver samples and cell monolayer; cloning; transient transfection; gene reporter assay- Cell culture: isolation and culturing of primary bovine and swine hepatocytes, isolation of biomatrix from bovine liver for plate coating; proliferation test (MTT), vitality test (LDH) and subculturing of several human cell lines- Bioinformatics:open source tools for: phylogenetic analysis, statistical analysis, primers thermodynamic evaluation, plasmid and cloning design, chromatogram analysis

Faculty Of Health Sciences - School Of Pharmacy

- Cloning of ligand-binding domain as well the full-sequence of the bovine PXR and CAR nuclear receptors- Cell culture: handling and transfection of human cell lines

Veterinary Medicine Faculty Of Padova

Post-lauream internship in the Pharmacology and Toxicology Laboratory of the Department of Public Health, Comparative Pathology and Veterinary HygieneMain task: Design of specific assays for end-point and qPCR

Veterinary Medicine Faculty Of Padova

Internship in the Pharmacology and Toxicology Laboratory of the Department of Public Health, Comparative Pathology and Veterinary HygieneMain task: qPCR (relative quantification), bioinformatic analysis

Veterinary Medicine Faculty Of Padova

Internship (bachelor’s degree) in the Pharmacology and Toxicology Laboratory of the Department of Public Health, Comparative Pathology and Veterinary HygieneMain task: Immunoblotting

Ph.D, Veterinary Sciences

Original Thesis: “Drug metabolizing enzymes and transporters in Bos taurus and cryopreserved pig hepatocytes: constitutive expression.

M.Sc., Biotechnology For Food Science, 110/110 Et Laude

Interfaculty of Agricultural Science, Medicine and Surgery and Veterinary Medicine – Orignal thesis: “Steroidogenic enzyme gene expression.

B.Sc., Health Biotechnology, 96/110

Interfaculty of Medicine and Surgery, Pharmacy and Veterinary Medicine – Original thesis: “Immunoblotting protocol optimization.