Vanessa Barcelo-Kreiger Email & Phone Number

United States

Philadelphia, Pennsylvania, United States

-Synthesis of protein-siRNA conjugates via site-direct conjugation for targeted genetic therapy.-Protein conjugates purification and characterization.-Method development.-In-vitro stability studies.

- Supported the development of new medical products for PPD clients in the pharmaceutical industry- Determined the concentration of excipients in antibodies drug conjugates (ADC) formulations using HPLC-ELSD- Studied the stability of protein-based drug products using capillary isoelectric focusing (cIEF)- Tested the binding ability of different ADC using Surface Plasmon Resonance and Biacore systems- Performed analysis of samples contained in medical devices like autoinjector prefilled syringes- Performed data processing and analysis- Reviewed and reported results- General trouble shooting

-Determination of precious metal concentration-Laboratory record keeping-Analysis of data and report of results

Miami/Fort Lauderdale Area

o Designed and executed the synthetic strategy to conjugate proteins to small targeting and fluorescent molecules, and polymers using amine-to-sulfhydryl crosslinkers via NHS-ester and pyridyldithiol reactive groups, as well as carbodiimide chemistryo Optimized the synthesis of a smart, targeted nano-sized drug delivery system for a therapeutic protein for cancer therapy. Covalently modified the Lys on the surface of the protein nanoparticles with polymers and targeting ligands o Used nanoprecipitation to obtain protein nanoparticleso Performed in-vitro studies of cell viability, drug internalization, apoptosis, and folic acid receptor expression in mammalian cellso Performed immunofluorescence and confocal microscopy imaging of several mammalian cells lineso Performed physicochemical characterization (including residual enzymatic activity, level of modification, size, surface charge etc.), of proteins protein conjugate and nanoparticles using fluorescence and UV/VIS spectroscopy, dynamic light scattering (DLS), zeta potential, optical, fluorescent, and confocal microscopy, among otherso Tested different excipients and lyophilization conditions to stabilize protein formulationso Used different types of filtration to separate different size polymer, proteins, and particleso Synthesized a block co-polymer for drug delivery using a catalyzed esterification reaction, purified it, and characterized it using nuclear magnetic resonance (NMR)

Puerto Rico

o Transformed e-coli BL21 (DE3) with a plasmid containing the DNA sequence of a His-tagged recombinant protein (Granzyme B)o Used fast protein liquid chromatography (FPLC) in combination with affinity chromatography, SDS-PAGE electrophoresis and Western Blotting to purify and characterize a His-tagged recombinant protein expressed in E. coli o Studied changes in the secondary and tertiary structure of several proteins using circular dichroism (CD)o Optimized the synthesis of nanoparticles of the proteins Ribonuclease A, Alpha-Casein and Lipase using a nanoprecipitation methodo Developed a chitosan nanocapsule for negatively charged proteins using electrostatic self- assemblyo Collaborated in the developed of a protein-based catalyst for biodiesel production o Collaborated in the assessment of protein/folate system as a treatment for gliomao Maintained bacteria and mammalian cell cultures o Research in active drug delivery targeting cancer, based in protein nanoparticles coated with polymerso Wrote the original proposal: "Study of the effect of blocking the CD38 receptor with adenosine diphosphate ribose (8-Br-cADPR) in ischemic brain"o Presented the graduate seminar: "Induced Selective Degradation of Proteins Via Modulation of The E3 Ubiquitin Ligase Complex CRL"o Mentored, supported, and supervised 6 undergraduate studentso Mentored one Visiting student during the 2017 Research Experiences for Undergraduates (REU) at University of Puerto Ricoo Authored three peer-reviewed publications

o Prepared and supervised laboratory sections of organic chemistryo Presented short lectures on organic chemistry theory to undergraduate studentso Reviewed and graded laboratory reports and exams

Grupo De Investigación En Compuestos Heterocíclicos. Barranquilla, Colombia

o Synthesized and characterized (NMR, IR, and MS) the APIs 2-alkyl tetrahydroquinolines with antifungal activity using multicomponent Imino Diels-Alder reactions o Tested different crystallization conditions to purify the product of organic synthesis

Barranquilla, Colombia

Prepared solutions, classified and organized reactants and handled UV-Vis spectrophotometer

La Loma, Colombia

o Performed physicochemical analysis of water and suspended particulate matter in airo Worked following strictly regulated SOPs and laboratory conditionso Monitored bacteria presence in different samples of water

Doctor Of Philosophy (Phd), Chemistry/Biochemistry

Chemist, Chemistry