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Area of expertise: Molecular Biology, cell Biology, Biochemistry and VirologyLaboratory ManagementCell-based assaysAssay DevelopmentIHCProtein-protein / DNA interaction Flow Cytometry Fluorescence microscopyELISARecombinant protein including therapeutic monoclonal antibodies expression and purificationDoE in mammalian cell (including 3D), mice and human viruses (HCV, HIV and Influenza)RNAi, Transduction, Transfection ChromatographyNMR and mass spectroscopyNative PAGE, SDS-PAGEWestern BlotPosttranslational modification EMSAAutoradiographyHPLC, MS-MS, NMR
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Postdoctoral Scientist - Drug Discovery And Chemical BiologyUniversity Of Basel Nov 2015 - Dec 2016Department Of Organic Chemistry, University Of Basel- PROTACs (Proteolysis Targeting Chimera) synthesis, validation- Small molecule multi-target drugs synthesis, purification (column chromatography, HPLCs, Isolera), characterization (UPLC-MS, ESI-MS, HPLC, NMR) - Management and development incahrge - molecular and cell biology unit- Protocol development and drugs screening in mammalian cells- NEBuilder HiFi DNA (Gibson) Assembly of multifragment of DNA - Cells staining (living / fixed - permeabilized) and multicolor fluorescent microscopy, Western blot, cytotoxic assay, qPCR- Training colleagues -
Postdoctoral ScientistMemo Therapeutics Ag, Zurich / Eth, Zurich Nov 2012 - Nov 2014Basel Area, Switzerland- High resolution assay and analytics development for the high-throughput discovery and characterization of therapeutic antibodies- 3D gel cells (B / CHO) culture and high-throughput screening system development- Microfluidics based micro-encapsulation of cells in 3D emulsion droplets and RT-PCR- Laboratory management -
Phd BiochemistryUniversity Hospital Basel, University Of Basel Sep 2007 - Sep 2012Basel Area, Switzerland- Viral Hepatitis C and innate immunity- Hepatocellular carcinoma- Peptide nanoparticle invention for genes and drug delivery into cells and tissues- Genetic variants and microRNA analysis- Cell lines and mice strain generation -
Project AssistantNii - National Institute Of Immunology Jun 2006 - Aug 2007New Delhi Area, India- Functional characterization of genetic variants of a selective attractant for memory T lymphocytes and monocytes (RANTES; Chemokine)- Genetic mutation analysis of HIV-1 receptor CCR5 ORF- Gene therapy against influenza using combinatorial approach - DNAzyme, Ribozyme and siRNA -
Msc Biotechnology & Project StudentUniversity Of Hyderabad 2002 - 2005Hyderabad, India- Xenobiotoxicity of environmentally and industrially relevant organic molecules by bacteria- Purification of secondary metabolites and biochemical pathway determination
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Frequently Asked Questions about Vijay Shanker
What is Vijay Shanker's role at the current company?
Vijay Shanker's current role is Business Partner at Rubix108 Technologies.
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What skills is Vijay Shanker known for?
Vijay Shanker has skills like Molecular Biology, Biochemistry, Western Blotting, Cell Biology, Transfection, Protein Chemistry, Pcr, Cell, Flow Cytometry, Elisa, Protein Expression, Molecular Cloning.
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vijay shanker
Lifestyle Omni Channel Retail Specialist With Focus On Growth & Profitability And A Bias Towards Action, Customer Obsession , High Performance Standards And Entrepreneurial ApproachNew Delhi1yahoo.co.in -
Vijay Shanker
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