Vinoth Kumar.K Email & Phone Number

Hyderabad, In

Telangana, India

Maintenance, expansion and cryopreservation of cultured cells; preparation of working cell banks. To assist in cell culture process development/Scale up/Tech transfer for mAb’s project.including media screening, cell adaptation and growth optimization. Handling of single-use wave bioreactor (50-200L), handling of 10L glass bioreactor (Sartorius), handling of shake flasks (125ml-5L).  Worked in tech transfer, manpower management; preparation of SOPs, BMRs, and MFRs.… Show more Maintenance, expansion and cryopreservation of cultured cells; preparation of working cell banks. To assist in cell culture process development/Scale up/Tech transfer for mAb’s project.including media screening, cell adaptation and growth optimization. Handling of single-use wave bioreactor (50-200L), handling of 10L glass bioreactor (Sartorius), handling of shake flasks (125ml-5L).  Worked in tech transfer, manpower management; preparation of SOPs, BMRs, and MFRs. Monitoring reactor and recording parameters. To perform media, feed preparation, filtrations, aseptic handling techniques, cell sampling, and cell counting. Show less

Bengaluru, Karnataka, India

 Mammalian cell culture development from 50L seed bioreactor to 9KL production bioreactor & Human insulin development from seed to production level (30KL). Single use bioreactor operations- Pre checks, Bag Inflation, Media intake andinprocess monitoring (50L &200L) SS bioreactor operations- CIP, PHT, ESIP of bioreactors, Media intake and Inprocess Monitoring (1KL & 6KL) Bioreactor operations, bio welding, sealing and connection preparations. In process… Show more  Mammalian cell culture development from 50L seed bioreactor to 9KL production bioreactor & Human insulin development from seed to production level (30KL). Single use bioreactor operations- Pre checks, Bag Inflation, Media intake andinprocess monitoring (50L &200L) SS bioreactor operations- CIP, PHT, ESIP of bioreactors, Media intake and Inprocess Monitoring (1KL & 6KL) Bioreactor operations, bio welding, sealing and connection preparations. In process sampling and their analysis (Offline pH, offline pCO2, Osmolality,Viable cell concentration and viability Real time completion of BMR and supporting documents. Preparation of SOPs, protocols, qualification documents, reports, QMS elements.(GDP and cGMP). Equipment handle- Bio Metabolite Analyzer, Automatic cell Counter, Osmometer, Integrity Machine, Phase Contrast Microscope. Involving in PLC, SCADA system operations and DCS system operations.(Allen-Bradley, FTSE, ABB) Media & Feed preparation, Sterilization (Media & Filters) Tyndallization process for contaminated batch. Performing SIP/CIP for fermenters and associated vessels/tanks. Performing basic process & equipment troubleshooting. Performing sample collection for testing at all stages of fermentation. Show less

Bangalore

Coimbatore Area, India