• Investigated preclinical efficacy of Measles vector-based MERS-Corona vaccine (a CEPI project).• Multicolour intracellular cytokine staining (ICS) by flow cytometry, ELISA, cellular immune response by ELISpot, western blot, antigen expression by RT-qPCR, viral growth-kinetics and IPMA.• Completed FELASA-B certification • Conducted experiments in BSL-2 and BSL-3 laboratories. • Additional experience includes routine cell culture, virus cultivation, gene cloning, DNA and RNA… Show more • Investigated preclinical efficacy of Measles vector-based MERS-Corona vaccine (a CEPI project).• Multicolour intracellular cytokine staining (ICS) by flow cytometry, ELISA, cellular immune response by ELISpot, western blot, antigen expression by RT-qPCR, viral growth-kinetics and IPMA.• Completed FELASA-B certification • Conducted experiments in BSL-2 and BSL-3 laboratories. • Additional experience includes routine cell culture, virus cultivation, gene cloning, DNA and RNA preparations, viral gene sequence analysis. Show less

• Research focused on understanding role of APOBEC3G-regulated host factors REDD1 and KDELR2 in restriction of measles virus replication.• Extensive cell culture, PBMC isolation, Measles Virus cultivation, confocal and Immunofluorescent microscopy of virus infected cells, flow cytometry, cellular protein phosphorylation analysis by western blot, Co-IP of cellular and viral proteins, plaque assay, gene cloning, sh-RNA and si-RNA mediated gene silencing, PCR, and qRT-PCR.

• Part of National Task Force for laboratory containment of poliovirus.• Successfully prepared national database for containment of poliovirus involving more than 10000 potential laboratories in India • Prepared survey documents for laboratories working/storing any Poliovirus or related materials.

Experience • Media preparation, Maintenance of cell lines • Virus stock preparation and titration by cyto pathic assay (TCID 50), plaque assay, mice inoculation (LD50) • Establish In-vitro screening assay for in-house natural product metabolite library for identification of potential hits against Influenza virus, Herpes Simplex virus and Dengue virus • Primary screening and Dose response study of in-house NCEs • Mode of action studies of Herpes Simplex virus with… Show more Experience • Media preparation, Maintenance of cell lines • Virus stock preparation and titration by cyto pathic assay (TCID 50), plaque assay, mice inoculation (LD50) • Establish In-vitro screening assay for in-house natural product metabolite library for identification of potential hits against Influenza virus, Herpes Simplex virus and Dengue virus • Primary screening and Dose response study of in-house NCEs • Mode of action studies of Herpes Simplex virus with two leading anti-herpes herbal extracts • Determine synergistic activity of synthetic biomarkers of lead compounds • Development of animal models to determine In-vivo efficacy of lead compound in pre-clinical studies • Regular maintenance of lab journal, presentations of results in weekly review meetings.Achievements • Development of acyclovir resistant HSV-1 strain in-house • Evaluating the combination effect of biomarkers identified against HSV-1 and HSV-2 • Standardization of biochemical assay for the development of neuraminidase inhibitors against influenza virus Show less