Like ClinicalTrials.gov, ReBEC is dedicated to trials conducted within Brazilian territory, involving both Brazilian and foreign researchers:• Proficiency in regulatory compliance through the meticulous review, approval, and monitoring the clinical study records submitted to ReBEC• Managed complex records of research and registration elements, ensuring their comprehensive compilation and accessibility for audits by Federal regulatory agencies, community, and researchers• Generated statistical and administrative reports to ensure ReBEC compliance, and authored the register manual to streamline the submission process for clinical study records• Provided interpretation, guidance, and educational support to researchers, facilitating the registration, maintenance, monitoring, and results reporting of research studies within ReBEC• Provided comprehensive training to new team members to skillfully assess, approve, and oversee clinical study records submitted to ReBEC

• Designed and implemented multidisciplinary research projects and collaborated with intramural and extramural researchers.• Study design: Collaborated in an experimental protocol to measure Forearm Blood Flow (FBF) responses through intra-arterial infusions of acetylcholine and sodium nitroprusside in adults with spinal cord injury and adults with periodontal disease, utilizing strain-gauge plethysmography.• Executed numerous mammalian cell experiments involving the treatment of cells with endothelial microvesicles isolated from adults with obesity, individuals with spinal cord injury, and e-cigarette users.• Technical skills: Cultivated Human Endothelial Cells: HUVECs, HPAEC, HCAEC, hCMECs, and cardiomyocyte in culture media.• Sorted and collected endothelial microvesicles (EMVs) by fluorescence-activated cell sorting.• Quantified intracellular proteins (e.g. eNOS, NF-KB) and plasma endothelin-1 (ET-1) concentrations using Bio-Rad DC protein assay and ELISA assay.• Performed RT-PCR to determine circulating and intracellular microRNA expression for specific miRs associated with cellular inflammation, apoptosis, and cardiovascular risk.• Data analysis: Performed statistical analyses, including student's t-test, Wilcoxon rank test, Mann-Whitney test, Chi-squared test, ANOVA, Post-hoc tests, Cohen’s d, and Pearson's correlation, linear regression, multiple linear regression.• Accomplishments: Developed a laboratory protocol for freezing and growing cells that significantly increases the number of cells available for each experiment, leading to significant cost savings of $102,200.00.• Published 6 papers in peer-reviewed journals, 3 abstracts for national conferences, and won 2+ travel awards to attend national scientific meetings.• Mentored 8+ master’s, undergraduate, and high school students in the culturing of mammalian cell cultures, extracellular vesicles isolation, and protein quantification.

•Designed and implemented multidisciplinary research projects and collaborated with intramural and extramural researchers.• Study design: Conducted mammalian cell studies, applying drugs sourced from natural compounds like Endothelin-1 and IκB kinase [IKK] inhibitor.• Executed diverse mammalian cell experiments involving the treatment of cells with extracellular vesicles isolated from Andean highlanders with excessive red blood cells and individuals with spinal cord injury. • Technical skills: Employed ELISA assays to measure specific circulating biomarkers related to coagulation (von Willebrand factor, factors VII, VIII, and X), the fibrinolytic system (tissue-type plasminogen activator, plasminogen activator inhibitor-1), and fibrin formation (D-dimer).• Utilized flow cytometry (model BD FACSAria II) to isolate and characterize circulating endothelial microvesicles (EMVs) from human blood samples.• Executed in vitro oxidative stress assays using the CellROX® Deep Red reagent.• Conducted cell viability assays (MTT) to assess cytotoxicity induced by natural compounds.• Accomplishments: Published 4 papers in peer-reviewed scientific journals and 3 abstracts for national scientific meetings

• Study design: Managed complex clinical research trials with human participants, employing randomized, double-blinded, placebo-controlled crossover designs.• Technical skills: Isolated and characterized endothelial progenitor cells (EPCs) and platelet-derived microparticles from blood samples using flow cytometry.• Measured plasma concentrations of angiogenic factors (VEGF and SDF-1) and assessed neuronal-parenchymal damage through the quantification of neuron-specific enolase using ELISA assays.• Conducted in vitro oxidative stress assays, including TBARS, 8-isoprostane, protein carbonylation, and superoxide dismutase, and catalase.• Evaluated coagulation parameters by Prothrombin time (PT) and active partial thromboplastin time (aPTT) assays.• Knowledge in multiplex bead array assay, applied for the measurement of human plasma cell adhesion molecules (ICAM-1, VCAM-1, and P-selectin), fibrinolytic markers (tPA and PAI-1), and inflammatory cytokines (IL-1b, IL-6, IL-8, IL-10, MCP-1, and TNF-α).• Data analysis: Performed statistical analyses, including Student's t-test, Wilcoxon rank test, Mann-Whitney test, ANOVA, Post-hoc tests, Cohen’s d, and Pearson's correlation, to compare clinical variables between groups.• Accomplishments: Published 12 papers in peer-reviewed scientific journal, 2 abstracts for national and international scientific meetings, and won 1 honorable mention award. • Mentored 2 undergraduate students in technical, communication, and presentation skills.

• Clinical trial: Facilitated the informed consent process, ensuring participants’ understanding and willingness to participate in the clinical trial• Study design: Conducted and supervised a pharmacological clinical trial study, including a 10-minute baseline period and 30 minutes of intravenous administration of l-arginine or saline.• Handled biological human samples by collecting, processing, and storing blood samples in accordance with standard protocols.• Technical skills: Performed ozone-chemiluminescence method to measure serum nitrite levels.• Conducted gelatin zymography and ELISA assays to determine metalloproteinase activity and TIMP-1 concentration in the serum of patients with hypertension.• Experienced with oxidative stress assays (TBARS and 8-isoprostane). • Data Analysis: Performed statistical analyses, including Student's t-tests, Mann-Whitney tests, and two-way repeated measure ANOVA, and Pearson's correlation, to compare circulating plasma biomarkers between groups before and after the infusions.• Accomplishments: Published 1 paper in a peer-reviewed scientific journal, 3 abstracts for scientific meetings in Brazil, and won the Young Investigator Michel Jamra Award.

• Clinical trial: Experienced in subject’s recruitment for research studies• Assisted with exercise protocols in 2 clinical trial studies focused on measuring the impact of eNOS polymorphisms on the cardiovascular system and investigating blood pressure response to cycling in hypertensive men.• Technical skills: Performed immunoturbidimetric assay to measure serum High-sensitivity C-reactive protein levels (hsCRP).• Conducted analytic methods (PCR and RT-PCR) to identify polymorphisms in the eNOS gene.• Experienced with Genomic DNA extraction from circulating leucocytes. • Data Analysis: Conducted stratified analysis to determine the impact of the number of Metabolic Syndrome risk factors on serum (hsCRP) levels in women and men. • Accomplishments: Published 3 papers in peer-reviewed scientific journals, 3 abstracts for scientific meetings in Brazil, and 2+ honorable mention awards.

• Study design: Designed and executed in vitro experiments involving the treatment of mammalian cells with natural compounds such as glucose, insulin, L-NAME, TMAO, estradiol, and HMGB1, as well as extracellular vesicles isolated from adults living with HIV.• Collaborated on the experimental protocol for a study on the impact of habitual short sleep duration (<7 hours/night) on circulating microRNA expression.• Technical skills: Cultivated Human Umbilical Vein Endothelial Cells (HUVECs) in culture media. • Performed isolation and identification of circulating extracellular vesicles from human plasma using a flow cytometry.• Conducted in vitro oxidative stress assays via detection of 2′,7′‐dichlorofluorescin diacetate.• Utilized automated electrophoresis immunoassay (Wes-ProteinSimple) for intracellular protein expression analysis.• Quantified the concentration of interleukin-6 and interleukin-8 in media from extracellular vesicles-treated cells using ELISA assays.• Extracted RNA from extracellular vesicles-treaded cells and human plasma.• Performed RT-PCR to determine circulating and intracellular microRNA expression for specific miRs associated with cellular inflammation, senescence, apoptosis, and cardiovascular risk.• Accomplishments: Published 2 papers in a peer-reviewed scientific journal and 1 abstract for a national scientific meeting.