Wang Xiang Email and Phone Number

• Participated in developing and improving the ROOT (Revealing Origins and Ontological Targets) algorithm pipeline for drug repositioning. The compound prioritized was proven effective in rejuvenating quiescent neural stem cells and improving cognitive function, in vivo and in vitro. Publication is under submission. • Performed comprehensive analysis on unmasking the mechanism and target of the drug prioritized by ROOT. Including integration of external ATAC-seq and Chip-seq dataset analysis and 10x genomic scRNA-seq data analysis on drug-treated/untreated mice hippocampus. Transplanted ROOT pipeline into other compound prediction situations, collaborated with Dr.Cobrinik Lab, Children Hospital of Los Angeles.• Performed 10x genomic scRNA-seq data analysis pipeline, executed downstream analysis on exploring the interaction between air pollution and AD risk with premature aging of neural stem cells. Led undergraduate students to build an interactive shiny app for data visualization for better collaboration with teams with different backgrounds. Grants are successfully funded by NIH. • Synthesized in vivo single-cell lineage tracing and multiple systematic computation approaches, including WGCNA and pseudoage in sc-RNA seq data, demonstrate that exercise enhances neural stem cell (NSC) function, promoting neurogenesis and self-renewal, mitigating early molecular aging, and reducing Alzheimer's disease risk. Co-first autor publication is under submission. • Provided a transcriptomic landscape on mice hippocampus neurogenic niche, focusing on early molecular aging processes and exercise interventions. First-author publication is in preparation.• Analyzed human hippocampus patch-seq data and 10x single-cell RNA-seq data to investigate adult astrogenesis and its role during mesial temporal lobe epilepsy progression. The result was presented in the USC-Buck Biology of Aging Seminar Series.

• Participating in writing a review of mitochondrial ORF published in Exp. Cell Res, focusing on the genomic and therapeutic potential of peptides derived from mitochondrial ORFs.• Employed data regression models for quantitative measurement of age-related height loss; utilized Genome-Wide Association Studies (GWAS) and polygenic scoring to identify potential gene loci and explore their relationship with aging and other phenotypes.• Conducted GWAS on the mitochondrial genome, identifying SNPs related to human height; isolated relevant mitochondrial peptides and conceptualized in vitro experiments for validation.• Assisted in securing a P01 grant focused on the relationship between Alzheimer's Disease, mitochondria peptide, and diet; responsible for RNA-Seq data exploration and analysis, selected mitochondrial peptides, and designed both in vivo and in vitro experiments to assess their effects on Alzheimer's Disease.• Assistant in COVID-19 mitochondria transcriptome analysis project during COVID, study focused on analyzing the distinctive mitochondrial transcriptomic changes induced by SARS-CoV-2 infection.

• Co-founded Make Sense Education Technology( Now re-brainding as ResoRoute) Co.Ltd, collaborating with a diverse team from top global universities.• Coordinated the development of the Bridge platform. The platform facilitated connections between students and global educational opportunities such as conferences, competitions, and self-improvement programs to promote access to high-quality resources. • Earned a spot on the shortlist for the renowned 2019 Y Combinator incubator program.• Organized and hosted the International Technology Innovation and Sustainable Development Summit, coordinated academic resources for the summit, fostering collaborations and promoting academic development. • Worked as KOL for IP “mv学姐”,( means senior M and senior V(me)) and has fans over 30k in one year(2019), including Bilibili, Tic Tok, Ixigua, etc. collaborated with ViaX, Zonglv Dadao, and multiple education institutes. Assistant for operation on the IP-related association with around 2k members, • Assistant in creating another self-media account in Red, “小军师M姐” ( means counselor Ms.M). Established and managed the "莠妙解" (means we have solution) and “The SuperSonic Club” communities, fostering a collaborative environment for shared knowledge and resource exchange.• Collaborating with Global Entrepreneurship Bootcamp, assisting with the talent selection and advertisement in China Mainland.

1.Identification of lncRNA Function Based on Large-scale Gene Disturbance Resources● Visualization of human chromosome 1 conservation using the Hilbert curve R package● Reviewing studies about lncRNA function predictions● Empathic learning and attending the discussions2.Identification of lncRNA Effects on Acute Cerebral Ischemia Stroke Based on the ceRNA Network● Pre-processing the RNA-Seq Data of stroke samples and control samples from GEO and performing a comprehensive analysis● Performing a 4-step method to construct two ceRNA networks based on case samples and control samples● Comparing the two networks and identifying the most rewired lncRNA nodes in the networks, and detecting its effect on ischemia-reperfusion injury● Assistant on experimental validation part using the lncRNA knocked-out mouse in vivo and the neurons and the endothelial cell lines in vitro3.Identifies lncRNA Functions Based on Gene Expression Bayesian Network in Human Epileptic Hippocampus● Re-annotation the microarray data and performing a comprehensive analysi● Constructing a Bayesian network and analyzing the network using module mining, WGCNA, enrichment analysis. etc● Discover the potential relationship between immunology and pathophysiologic mechanism of epilepsy, focus on ion channel gene which could cause abnormal electrical activity4.Identifies lncRNA Functions Based on ceRNA Network in Human Epileptic Hippocampus ● Re-annotation of the microarray data and perform comprehensive analysis from ceRNA perspective● Performing a method to predict the ceRNA relationship and constructing the ceRNA network

● Participating in the case discussion, assisting the doctor working on the case reports and ward rounds in the neurology department and oncology department● Collecting cases biopsy, making pathology section, and assisting immunohistochemical analysis in the pathology department● Participating in the case discussion and assisting the medical director in assigning a therapeutic schedule for ischemia stroke in the neurology department, the therapeutic schedule included antiplatelet/anticoagulation drugs, thrombolytic therapy, improved blood circulation, and neuroprotective effects.● Assisting and writing several pieces of research e.g. Effectiveness of Ultrasound-assisted Thrombolytic Therapy, Hyperuricaemia Acting as a Risk Factor for Ischemic Stroke, The Use of Contrast-enhanced Ultrasound Technique, in statistical analysis part