-BME 330 Engineering Principles of Molecules, Cells, and Tissues; Instructor Dr. Darilis Suarez-Gonzalez-Topics covered included concepts of reaction rate, stoichiometry, equilibrium, momentum/mass transport, and the interaction between transport and kinetics in biological systems.-Led weekly discussion sessions to review concepts and homework problems.-Supervised the Mc. Burney student alternative exam scheduling and graded all assignments.

Randolph Ashton's Stem Cell Bioprocessing Lab located in WID Madison, WI-Cultured and derived neural progenitors and cardiomyocytes from induced pluripotent stem cells (iPSCs).-Developed a vitrification method to create amorphous glass mediums for clinical stem cell banking, storage, and transportation of single-cell 2D suspensions and adherent 3D cell sheets.-Differentiated iPSCs to neuroectoderm and collected spent media for analysis of extracellular vehicles (EVs) for… Show more Randolph Ashton's Stem Cell Bioprocessing Lab located in WID Madison, WI-Cultured and derived neural progenitors and cardiomyocytes from induced pluripotent stem cells (iPSCs).-Developed a vitrification method to create amorphous glass mediums for clinical stem cell banking, storage, and transportation of single-cell 2D suspensions and adherent 3D cell sheets.-Differentiated iPSCs to neuroectoderm and collected spent media for analysis of extracellular vehicles (EVs) for clinical and engineered construct applications.-Formulated cryopreservation mediums for iPSCs and iPSC-derived cells.-Analyzed the consequences of cryopreservation techniques and medium formulations on iPSC-derived cells.-Generated quarterly and annual data reports for the National Science Foundation (NSF).-Presented oral poster boards and seminars for NSF Engineering Center for Cell Manufacturing Technologies -Supervised two undergraduate research assistants. Show less

Urban Triage – Madison, WI Agriculture Non-profit with Herbal Aspect, LLC. Collaboration• Developed pilot course for endocannabinoid/hemp recreational and industrial growing in WI• Managed a team of three employees: two course administrators and one licensed hemp grower• Lectured 4-hours at night once a week on Mondays and supervised in-field growing days on Thursday and Saturday evenings classes to a cohort of 12 minority entrepreneurs with a virtual hybrid… Show more Urban Triage – Madison, WI Agriculture Non-profit with Herbal Aspect, LLC. Collaboration• Developed pilot course for endocannabinoid/hemp recreational and industrial growing in WI• Managed a team of three employees: two course administrators and one licensed hemp grower• Lectured 4-hours at night once a week on Mondays and supervised in-field growing days on Thursday and Saturday evenings classes to a cohort of 12 minority entrepreneurs with a virtual hybrid component.• Developed curriculum about lean start-up business fundamentals: planning, entity creation, market research, financial statements, marketing, and funding; FDA regulations of hemp, biology of hemp, chemical processing techniques of hemp, and both hydroponic and soil growth of hemp at licensed farm space in Verona, WI.• Recruited and managed guest speakers and mentors, including logistics of their participation.• Attended weekly staff planning meetings and collected grant reporting data.• Six female black participants formed LLCs and went through required government permits to begin growing hemp legally in Wisconsin and selling products. Show less

The collaboration of Academic and Industry partners to transform the manufacturing of cell-based therapeutics into a large-scale, lower-cost, reproducible, and high-quality engineered process, for broad industry and clinical use.

• Led weekly out of class virtual tutoring sessions for BME 430: Biological Interactions w/ Materials• Created jeopardy games and exam review materials for in-class module review sessions• Supervised the graduate student research projects/presentations and graded all assignments• Instructor(s) Dr. Randolph Ashton and Dr. Tracy Puccinelli

Oversaw (6) hybrid business training sessions as head lecturer to 10 minority entrepreneurs for the Black and Brown Entrepreneur Center Business Incubator (B2EC) hosted by MBCC. I aided in tailoring the curriculum and facilitating the business development training, coordinating and managing stakeholders and events, and coaching participants. Curriculum development and training facilitation (40%): -Tailored (six) two-hour training modules to ensure cultural and social relevance.… Show more Oversaw (6) hybrid business training sessions as head lecturer to 10 minority entrepreneurs for the Black and Brown Entrepreneur Center Business Incubator (B2EC) hosted by MBCC. I aided in tailoring the curriculum and facilitating the business development training, coordinating and managing stakeholders and events, and coaching participants. Curriculum development and training facilitation (40%): -Tailored (six) two-hour training modules to ensure cultural and social relevance. Modules covered business fundamentals, including planning, entity creation, market research, financial statements, marketing, and funding.-Reviewed core course content w/ co-facilitator and delivered six three-hour training sessions through Thinkific and Zoom, with potential for onsite participation dependent on social distancing restrictions.-Created an inclusive, respectful, safe space to generate honest/meaningful discussions concerning minority entrepreneurs.Coordination and management (30%): -Recruited and managed guest speakers and mentors, including logistics of their participation. -Kept session and participants on track to achieve session, B2EC, and participant objectives-Scheduled and organized B2EC networking and graduation events. -Attended weekly staff planning meetings and collected grant reporting dataCoaching participants (30%): -Supported participants through the business planning process: helping them refine ideas, referring them to available resources, and serving as an accountability partner to ensure they emerge from the incubator with a viable business plan in hand. -Responded to participant inquiries within one business day. -Reviewed participant applications, business plans via LivePlan, and CVs. Show less

Offered an opportunity for doctoral students to obtain specific training and experience in the ever-growing field of biotechnology, complete with focused coursework, an industry internship, and funding for up to two-five years.

Global R&D - Science, Tech, Capabilities under Ben Minerath (team management), Karien Rodriguez (team lead), Lindsey Peed, and Su-Chon.1. Skin hydration and barrier function: pilot infant diaper arm band study using ATR-FTIR tools -Created and validated protocols for transdermal epidermal water loss (TEWL) measurement -Due to COVID restrictions of infant clinical studies, collected and analyzed TEWL ATR-FTIR data from adults subjects to compare the prolonged… Show more Global R&D - Science, Tech, Capabilities under Ben Minerath (team management), Karien Rodriguez (team lead), Lindsey Peed, and Su-Chon.1. Skin hydration and barrier function: pilot infant diaper arm band study using ATR-FTIR tools -Created and validated protocols for transdermal epidermal water loss (TEWL) measurement -Due to COVID restrictions of infant clinical studies, collected and analyzed TEWL ATR-FTIR data from adults subjects to compare the prolonged use of top commercial diaper brands 2. Healthy skin microbiome: multilayered model of the human dermis and epidermis -Maintained and conducted MatTEK EpidermFT skin models to compare the effect of top commercial baby wipe solutions on infant/adult microbiome overtime, and pre- postbiotic blends. -Luminex assay used to quantify over 10 cytokines and markers related to skin inflammation, death, and proliferation -(BCG) Albumin assay and immunohistostaining also conducted3. Technical writing, presentations, and communication skills -Attended international strategy calls for global skin clinical trial initiatives/collaborations -Presented weekly/monthly data sets to the human physiology and healthy diapered skin/microbiome teams -Presented end of co-op term presentation ~45min to whole R&D -Wrote 5 in-house reviewed and published technical literature reviews for the North American infant skin, microbiome, and feminine hygiene R&D and Business units entitled: 'Skin development', 'Infant skin development', 'Prebiotics', 'Postbiotics', 'Cannabidiols (CBD) skin and feminine hygiene applications' Show less

-Designed and optimized a systems biology-based protocol to explore Abiraterone-combination based therapies for enhanced efficacy in cancer cell cytotoxicity.-Worked to identify mechanisms of resistance to androgen receptor-targeted therapies in Dr. Michael B. Yaffe’s biological engineering lab.

• Created and maintained a residence hall environment that was conducive for living and learning. • Submitted incident reports, conducted bi-weekly programs for residences, and created interactive learning bulletin boards every month.

-Validated a phenotyping antibody panel and optimized a T-cell differentiation protocol with the goals of applying to the clinical samples of multiple sclerosis patients in Dr. Christopher Love's Lab. -Acquired skills in RNA sequencing, flow cytometry, tissue culture, microengraving, and nano-well bioanalysis.

-Collaborated with engineering peers to design and implement a biogas chamber and water gauging system in Dangriga, Belize.-Built a hydroponic system at a local high school and mentored students for careers in engineering-Supervisor: Dr. Findley Edwards UARK Department of Civil Engineering

-Developed a single disinfectant protocol to assess organic precursors of disinfectant-by-products in natural waters, and modified granular activated carbon to decrease the presence of disinfectant-by-products in filtered water. -Presented at the 2015 Arkansas Water Works Conference and won first place in undergraduate research presentation. Gained skills in sterilizing glassware, filtering water, buffering and titrating samples, extracting samples from Beaver Lake in Oklahoma, and UV/V… Show more -Developed a single disinfectant protocol to assess organic precursors of disinfectant-by-products in natural waters, and modified granular activated carbon to decrease the presence of disinfectant-by-products in filtered water. -Presented at the 2015 Arkansas Water Works Conference and won first place in undergraduate research presentation. Gained skills in sterilizing glassware, filtering water, buffering and titrating samples, extracting samples from Beaver Lake in Oklahoma, and UV/V spectroscopy. -NSF Career Grant #1254350 supported my study abroad to Dangriga, Belize to build a water gauging system with other engineering students. Show less