Jonathan Wee Email & Phone Number

Singapore

Singapore

• Testing and characterization of sequencing systems: including hardware, software, reagents and consumables to support the development of next generation platforms• Analysis and visualization of test data to identify root causes of technical issues• Quickly deploy solutions or mitigations to support product development

Singapore

Characterized a diet-induced mouse model of Non-alcoholic fatty liver disease (NAFLD)• Optimized protocols and sample preparation for flow cytometry and single cell RNA sequencing• Mentored junior Ph.D. students and research officers

Studied the role of the extracellular matrix protein Angptl4 in wound macrophages• Profiled immune cells during wound healing with flow cytometry and 10X Genomics single cell transcriptomics• Analyzed high dimensional single cell RNA-seq data with bioinformatics tools• Elucidated molecular pathways of wound healing with molecular and cell-based assays• Characterized the immune response in Angptl4 deficient wounds and identified its parallels with chronic diabetic wounds• Worked extensively with in-vivo mouse models and managed 4 transgenic mouse colonies• Designed, troubleshooted and executed experiments independently to tackle research problems

• Supervised and mentored 5 undergraduate students toward successful completion of final year research projects• Organized and taught experimental biology workshop in flow cytometry and histology for junior college students• Taught undergraduate tutorial classes and practical modules on histology methods, accumulated 200 hours of teaching duties• Established teaching materials and video recordings for undergraduate e-learning

Studied the role of the Kindlin family of focal adhesion proteins and their role in cancer cell migration and metastasis• Designed and cloned plasmids for the expression of mutant protein constructs• Performed genetic manipulation in cell lines for the expression of recombinant proteins, siRNAs and lentiviral particles• Developed live cell imaging, immunofluorescence assays and image processing protocols to study protein localization and cell membrane dynamics• Discovered that aberrant expression of Kindlin-3 in cancer cells impedes cell migration and metastasis due to aberrant activation of Rho GTPases

Doctor Of Philosophy - Phd

Appointed Graduate Research Officer by School of Biological Sciences, NTU. Pursued PhD degree as full time NTU staff.

Bachelor Of Science - Bs, Biological Sciences, First Class Honors

Performed 2 years of cell biology and oncology research under prestigious Undergraduate Research Experience on Campus (URECA) programme.