I am a seasoned mycologist with extensive experience in Industrial Biotechnology. Working initially in the fields of classical genetics & ecology of basidiomycete fungi bestowed on me a sound footing, which, along with the insights I gleaned from experimental analyses of fungal physiology (Serpula lacrymans) & molecular genetics of pathogenic (Phytophthora & Fusarium) & saprophytic (Phanerochaete, Trichoderma & Aspergillus) fungi, has endowed me with a sound & well-rounded appreciation of fungal biology. This perspective allows me to frame questions in a broad context, and often to formulate non-obvious approaches for achieving experimental goals & targets. I also have experience in starting & running two small (non-science) businesses, as well as experience as a customer services specialist.Key Roles Include:Project leader – Fungal host & production strain developmentRegulatory Document Preparation - US and EU documentation for strain and product approvals, including GRAS approvalCurator Fungal & Plasmid CollectionsDatabase Design & Maintenance (File Maker Pro, Web-based & In House)Small Business OwnerSales & Customer Service.Key Technologies & Skills:Yield improvement strategiesStrain construction & optimizationSelectable marker developmentDevelopment of methods for deletion, disruption or insertion of genes, leaving unmarked strainsPromoter/terminator evaluationsHigh-level expression Gene deletionsGene silencing using siRNAsOptimization of bench scale fermentation seed culture preparationSpecies Specific RAPD PCR Primer DesignKaryotype DeterminationPhenotype Microarray (PM) AnalysisSpectroscopic & Fluorescence Target DetectionDroplet digital PCR (ddPCR) for Target DNA QuantificationPCR, RT-PCRLibrary Generation & Vector ConstructionSequential Simplex OptimizationMutagenesis (Chemical & Physical)Cryogenic storageMicroscopy (including Fluorescence)Enzyme assaysELNs & LIMS