Wen-Hung Chou, Ph.D. Email and Phone Number

• Led biomarker discovery initiatives for cancer and aging by analyzing single-cell RNA-seq, spatial transcriptomics, and multiplexed proteomic imaging data • Contributed to projects profiling the immune and stromal microenvironment across multiple cancer types, including multiple myeloma, breast, and prostate cancer• Reconstructed 3D tumor structures by performing lightsheet microscopy of whole-mount immunofluorescence tumor samples and developed downstream image analysis protocols • Collaborated effectively with internal and external experts to advance team-based projects• Consistently delivered results within established timelines set by multi-institutional research consortia, including HTAN, SenNet, and GudMap

I study physical forces generated by the cell in the Gardel lab.• Developed computational pipelines and improved throughput for the quantitative analysis of high-resolution cellular imaging data using Matlab and Python• Designed and performed cell-based assays to study cytoskeletal dynamics by performing advanced microscopy on immunofluorescence/live-cell samples• Collaborated with cross-disciplinary scientists across institutions to design and develop interdisciplinary research plans that led to 2 research publications• Communicated research results to the research community with 2 manuscripts, 2 research proposals, and 10+ conference presentations• Mentored 2 undergraduate and 4 graduate researchers on project management, experimental design, wet-lab techniques, data analysis, and data presentation

Guided students to learn Python programming in the course "Quantitative Analysis of Biological Dynamics"• Co-devised content for computer lab sessions• Led computer lab sessions• Covered topics including numpy arrays, solving PDE, handling dataframes, and running regression models• Supported student growth by holding office hours

Advisor: Keng-hui Lin, Ph.D. (Institute of Physics)• Cultured quasi-3D cellular models of epithelial acini and 2D models of epithelial sheets to measure physical forces generated by confined epithelial cell colonies• Engineered micro-patterned substrates to control the morphology of 2D/3D epithelial cell colonies using soft lithographic techniques• Established software control of a custom-built epifluorescence microscope by connecting the microscope body, cameras, shutters, and light sources to micro-manager