Wesley Lim Email & Phone Number

California, United States

South San Francisco, California, Us

• Purified and normalized viral genetic material amplified via RT-PCR for Next Generation Sequencing, providing results ultimately used to aid patient treatment of HIV, HCV, and other viral infections, as well as for COVID-19 related research and development.• Processed high volumes of patient samples via automated liquid handlers utilizing molecular extraction techniques and RT-PCR• Performed extractions from transformed E. coli cultures, using spectrophotometry to quantify the extracted plasmids before delivering them for sequencing and transfection.• Assisted with the implementation of new tests, both developed in-house and FDA-Approved, by running validation samples and troubleshooting steps needing further attention.• Performed instrumentation and equipment preventative maintenance, sample inventory, and other routine lab duties, as well as troubleshooting for process disruptions.• Experience training new hires on full processes, both semi-automated and manual.• Experienced with data entry, test result interpretation, and determining sequential testing while working with clinical scientists.

La Jolla, Ca, Us

• Inoculated bacterial cultures using aseptic technique, practiced plate streaking and fermentation tests• Prepped DNA samples for Illumina sequencing, worked with miniPCR, brushed upon bioinformatics• Analyzed C. reinhardtii cultures using cell count with hemocytometer and spectrophotometry

La Jolla, Ca, Us

• Completed organic chemistry labs that required quickly learning new skills with short deadlines• Allocated work within a team to maximize efficiency within a small work space

La Jolla, Ca, Us

• Practiced Site Directed Mutagenesis on E. coli, tested mutation effects via RFP expression• Utilized RNAi gene knockout on C. elegans, tested expression effects via RT-qPCR

South San Francisco, Ca, Us

• Fulfilled daily product quotas and duties while simultaneously providing customer service needs

La Jolla, Ca, Us

• Applied DNA Extraction and Quantification, EcoPlate Biochemistry Assay, Gel Electrophoresis• Touched upon Sequence Analysis, Statistical Analysis, and Chi-Square of cumulative dataset• Worked with team members to compile findings into a paper for publication and a presentation for anaudience of graduate students and professors, ultimately aiding in a longitudinal environmental study