Wesley Lim Email & Phone Number

South San Francisco, California, US

• Purified and normalized viral genetic material amplified via RT-PCR for Next Generation Sequencing, providing results ultimately used to aid patient treatment of HIV, HCV, and other viral infections, as well as for.
• Processed high volumes of patient samples via automated liquid handlers utilizing molecular extraction techniques and RT-PCR
• Performed extractions from transformed E. coli cultures, using spectrophotometry to quantify the extracted plasmids before delivering them for sequencing and transfection.
• Assisted with the implementation of new tests, both developed in-house and FDA-Approved, by running validation samples and troubleshooting steps needing further attention.
• Performed instrumentation and equipment preventative maintenance, sample inventory, and other routine lab duties, as well as troubleshooting for process disruptions.
• Experience training new hires on full processes, both semi-automated and manual.

La Jolla, CA, US

• Inoculated bacterial cultures using aseptic technique, practiced plate streaking and fermentation tests
• Prepped DNA samples for Illumina sequencing, worked with miniPCR, brushed upon bioinformatics
• Analyzed C. reinhardtii cultures using cell count with hemocytometer and spectrophotometry

La Jolla, CA, US

• Completed organic chemistry labs that required quickly learning new skills with short deadlines
• Allocated work within a team to maximize efficiency within a small work space

La Jolla, CA, US

• Practiced Site Directed Mutagenesis on E. coli, tested mutation effects via RFP expression
• Utilized RNAi gene knockout on C. elegans, tested expression effects via RT-qPCR

South San Francisco, CA, US

• Fulfilled daily product quotas and duties while simultaneously providing customer service needs

La Jolla, CA, US

• Applied DNA Extraction and Quantification, EcoPlate Biochemistry Assay, Gel Electrophoresis
• Touched upon Sequence Analysis, Statistical Analysis, and Chi-Square of cumulative dataset
• Worked with team members to compile findings into a paper for publication and a presentation for anaudience of graduate students and professors, ultimately aiding in a longitudinal environmental study