William Lambeth Email & Phone Number

Kirkland, Wa, Us

Westbrook, Maine, Us

-Design & execute experiments & novel wet-lab workflows for biomarker discovery programs-Design qPCR & ddPCR assays for internal research use & commercial product launch-Prep RNAseq & DNAseq libraries for sequencing on multiple NGS platforms-Isolate nucleic acids from diverse & challenging sample types (plasma, WB, FFPE, FNA, fecal, etc)-Analyze data in JMP & other platforms, & consult w/ bioinformatics specialists for advanced insights-Present data & project updates at team & department level meetings-Provide training & guidance to new lab members on molecular diagnostic methods & best practices-Supervision & mentorship of Lab Associates tasked with sample management, purchasing & ordering, equipment maintenance, and general lab upkeep

Westbrook, Maine, Us

Westbrook, Maine, Us

Early discovery work on veterinary disease biomarkers - protein, small molecule, & DNA / RNA targets.-Established qPCR workflows for biomarker discovery projects including primer design, assay optimization, and RNA / DNA isolation-Cultured mammalian cell lines including primary, immortalized, stem cell, and hybridoma lines-Advanced cell culture techniques for biomarker discovery projects - 3D culture techniques, antibody and antigen production, electroporation & chemical transfection-Analyzed cell models and samples via fluorescence microscopy, SDS-PAGE, and Western Blot

-Assist in long-term selective breeding experiments on fruit flies (Drosophila melanogaster).-Set up experimental equipment, prepare standardized nutritional medium, and found new fly generations.-Conduct tests and collect experimental data on many individuals under controlled conditions.

-Perform DNA/ RNA extraction on samples, run appropriate PCR assays, and interpret results.-Conduct virology using cell culture techniques, and maintain a variety of animal cell lines.-Microbiology using general and selective media, staining techniques, and biochemical testing.-Assist in necropsy and sample collection from client submissions, and prep samples for analysis.

-Assisted in NSF-funded development of MicroPIE software for automatic extraction of microbial phenotypic information.-Selected and formatted data from published research papers for use to test software.-Helped develop Gold Standard to assess software performance, and correct computer output.

Westbrook, Maine, Us

-Worked under BSL-2 conditions using aseptic technique, PPE, and BSC.-Performed cell culture work involving E. coli (BL-21) and Canine Kidney Cells (MDCK).-Collected and interpreted OD600 spectrophotometer data for culture samples.