Will Roscoe Email & Phone Number

San Francisco, Ca, Us

South San Francisco, Ca, Us

• Conducted functional assays to characterize Tizona’s HLA-G antibody and bispecific antibody, assessing effects on ADCC, ADCP, cytokine production, target cell cytotoxicity and immune cell subtypes populations.• Executed cell-based assays to test internalization rate, protein clearance efficacy, and specificity of protein degrading fusion molecules.• Ran immunogenicity assays using T cells cocultured with PBMCs or MDDCs.• Constructed ELISAs to measure anti-drug antibodies (ADAs) in the serum of mice treated with therapeutic antibodies.• Studied effects of scavenger receptor blocking antibodies on chemokine uptake inhibition and immune cell migration.• Characterized antibody-drug conjugates (ADCs) by measuring compound internalization and target-specific cell killing.• Executed cell-based assays to test the internalization rate, target protein clearance efficacy, and specificity of protein degrading fusion molecules.

South San Francisco, California, Us

• Transduced, cultured, and conducted functional assays with CAR-T cells and chimeric engulfment receptor T-cells (CER-T) in collaboration with Lyell Therapeutics.• Monitored effects of CAR-T cells on target cell apoptosis and viability using Incucyte and flow cytometry.• Conducted serial stimulation assays to characterized exhaustion kinetics of CAR-T cells during co-culture with target cells.• Conducted T-cell based APC assays to test TAA presentation capabilities of CER-T cells.lls

• Evaluated immuno-oncology antibodies in cell-based assays to investigate their impact on cell surface protein expression, cytokine production, metabolism, and RNA synthesis. • Utilized CRISPR gene editing on T cells to knockout a receptor protein for which functional blocking antibodies were unavailable.• Engineered cell lines utilizing both human and cyno versions of DNA from lead antibodies and their family members to determine binding specificity and cross-species homology.• Developed biomarker assays employing cyno and human cells to identify patients responsive to lead antibodies.• Collaborated with BMS to characterize antibodies influencing the differentiation status of myeloid cells and their subsequent effects on T-cell proliferation and function.• Contributed to a collaborative project with Pfizer, screening the Five Prime protein library in a tumor stem cell assay to identify proteins affecting 3D tumor cell colony growth in agar-based cell culture media.• Conducted hydrodynamic tail vein transfections on mice to assess the effects of resultant proteins on implanted human xenograft growth. Collected blood, tissues, and organs from the study mice.

B.S., Biology

Certificate, Introduction To Immunology Course

Certificate, Clinical Trials Design & Management