William De Martini Ph.D. Email & Phone Number

Montgomery, Nj, Us

Montgomery, Nj, Us

Cranbury, New Jersey, United States

Participated in multiple projects for drug discovery of novel proteolysis targeting chimeras (PROTAC) and regulated induced proximity targeting chimeras (RIPTAC) compounds to treat various cancers. Successfully executed and troubleshooted various cell-based assays to determine potency of potential cancer drug therapies.Evaluated compound efficacy against targeted cancer protein using western blot, in-cell western and cell proliferation assays.Generated KO cell line models using CAS9 to investigate mechanism of anti-cancer compounds.

Princeton, New Jersey, United States

Successfully produced stable cell lines expressing various oncogenes (ex. FGFR, EGFR, HER and RET) using lentivirus transduction and piggbac technology.Handled multiple cell lines for cell line development including NIH3T3, HEK293T, BaF3 and multiple NCI and AML cell lines.Proficiently generated, concentrated and determined physical titer of lentiviruses used for stable cell line generation and calculated functional titer using qPCR.Expertly performed limiting dilutions and FACS to produce monoclonal cell lines.Regularly communicated project progression and presented data for clients over Zoom.Trained/supervised research associates to perform various techniques including mammalian cell culture, qPCR and western blotting. Generated SOPs in Cell Line Development group which aided on boarding process of research assistants/associates.

South Orange, Nj

Investigated the mechanism of a novel inhibitor of TNF𝛼-induced NF-𝜅B activation, MC163, encoded by the Molluscum Contagiosum Virus. Maintained several mammalian cell lines including HEK 293T, HeLa and RAW 267. Performed ectopic/transient expression of viral and host cell proteins to mammalian cell lines. Measured activity of NF-𝜅B and IFN-𝛽 using a dual reporter luciferase assay. Elucidated protein-protein binding partners through immunoprecipitation experimentation. Analyzed cells for key apoptosis events using confocal microscopy. Successfully trained incoming graduate and undergraduate for the above techniques. Presented research at ASV and ASM conferences though oral or poster presentations. Taught laboratory sections for General Biology and Cell Biology courses under the Department of Biological Sciences.

Montvale, Nj

Supported the Product Safety group in providing thorough human and environmental hazard evaluations and health risks assessments for Surface Care, Germ Protection, Medicinal and Personal Care products marketed globally.Communicated with R&D chemists globally on new and existing formulations in regards to generating safety assessment documentation (Product Safety Reports, Product Classification Files and Poison Control Center Information).Reviewed and assessed formulas and available animal in-vivo/in-vitro data under the appropriate EU/EC Directives/Guidelines (DPD, Detergent Directive, Cosmetic Directive, BPD and EU-GHS), US regulations (EPA, FDA and CPSC), Canadian (CCCR) and ASEAN Cosmetics.Ensured Precautionary and First Aid language Surface Care & Germ Protection product labels and packaging is appropriate and meets statutory requirements.Evaluated appropriate human clinical, in-vitro, and analytical studies to support product claims.Conducted assessments to ensure products meet California’s Proposition 65 requirements.Confirmed compliance with internal Regulatory Safety & Environmental policy, guidelines and procedures.Provided technical support to respond to consumer and legal inquiries concerning technical, safety and environmental issues for global RB products.

Contributed to research efforts involving the study of parasitic protein kinases homologous to p38 mitogen activated protein kinase (MAPK) and potency of drug compounds against a parasitic kinase using Immobilized Metal Ion Affinity Polarization (IMAP)Collaborated with the pharmaceutical company Celgene for drug screeningCultured cell lines HEK293F and CHO-S for transfection of recombinant proteinPurified GST tagged recombinant protein via column and measured enzyme activity via IMAPQuantified amounts of DNA and protein in samples via Hoechst and Bradford assays respectivelyExperimented with adult and immature stages of the filarial nematode Brugia malayi

Provided technical support for project to populate chemical database with data on raw materials from suppliers in order to provide safety and health hazards to customers.Reviewed MSDS's on raw materials for information such as toxicologically and physical characteristics.Proficient in usage of SAP software to extract and edit data from a large database.

Franklin Lakes, Nj

Executed numerous, thorough evaluations of incoming customer complaints of Class 1 and Class 2 medical devices (blood glucose monitors, syringes, lancets) under GLP guidelines. Major contributor in the overall removal of complaint backlog from 120+ day turnover to 60 days in Diabetes Care segment.Troubleshoot, maintained, and calibrated yellow spring instrument for glucose analysis. Conducted stability testing and competitive analyses on blood glucose test strips.Authored written reports on investigational findings including statistical analysis of data.Spiking of blood glucose samples for accuracy testing of blood glucose test strips.Created and maintained Access Database for data generated during accuracy testing of strip lots.Handled Failure Investigation Reports directed to the manufacturer/supplier for Quality Assurance resulting in the decision for corrective and preventive action.

Performed testing with the IMMULITE product line at the request of the Chemistry group manager.Operation and troubleshooting of high-throughput chemiluminescent immunoassay systems: IMMULITE, IM2K, IM2500.Liaison to Mechanical Engineering in evaluating newly designed modules, optimizing instrument performance.Assisted the group to validate a new mould of sample tubes produced by manufacturing to be released to the market. Completed all experiments under GLP guidelines.

Assisted in the project for the classification of Export Control Classification Numbers (ECCN) and Schedule B numbers of exported chemicals produced by Fisher Scientific according to guidelines of Government Regulations. Provided effective and efficient response to client inquiries and/or complaints regarding the company's products.Notification of product recalls to customers; Handled requests of customers for Material Safety Data Sheets (MSDS) and Certificates of Analysis.

Contributed to ongoing research studies involving various analytical chemistry and molecular biology laboratory techniques.; In vitro analysis of various conditions that effect lipolysis in human adipose tissue of obese through techniques such as the glycerol assay. Identification of lipoproteins such as perilipin and ADRP by SDS PAGE and Western Blotting.

Synthesis, reactions of quinolone derivatives.Isolation and purification using different forms of chromatography (e.g. tlc, hplc, Column), characterization of products by IR, NMR and Mass Spectrometry.

Doctor Of Philosophy (Ph.D.), Molecular Bioscience

Research Disseration: "Characterization of immune evasion properties of the Molluscum Contagiosum Virus MC163 protein."

Masters, Biochemistry

Activities and Societies: Sokol Institute of Pharmaceutical Life SciencesCompleted Research Thesis: "Combining bioinformatics and chemical.

Bs, Biochemistry/Nutrition