William Eng Email & Phone Number

New York, Ny

Greater New York City Area

Currently researching antibody based therapeutics to treat Acute Myeloid Leukemia.

Biomedical Chemistry Institute, Dept. Of Chemistry

Studied Galectin-3 (carbohydrate binding protein) related sorting of glycoproteins CD63 and Carcinoembryonic Antigen (CEA) and tetraspanin CD81 that are associated with Microvesicles/Exosomes from cell line SK-Mel-5. Used α-mannosidase I inhibitor, 1-deoxymannojirimycin (DMJ) in SK-Mel-5 cell culture in order to manipulate the glycan profile of the glycoproteins and therefore, examine the effects on Galectin-3 related protein sorting between the cell membrane and corresponding Microvesicles. Managed the distribution of commercially obtained biotin conjugated lectins and related reagents for the Consortium of Functional Glycomics (CFG) comprehensive lectin binding study. Biotin labeled and then ELISA tested selected lectins designated for the CFG binding study. Printed lectin microarrays using the GeSim Nano-Plotter NP 2.1, for use in the glycomic analysis of various biological samples; familiar with GenePix Pro 7 microarray acquisition/analysis software. Managed lab supplies, biochemical reagents and general equipment.

Expressed and purified neuronal regulatory protein, Complexin (Cpx) 1 for cell based and liposome based fusion assays. Engineered and expressed constructs for protein Cpx 3 in the pET-SUMO vector system. Purified wild type and mutant forms of Cpx 3 protein for various functional studies. Performed binding studies of Cpx 1 and Cpx 3 proteins using reconstituted SNARE (vesicle fusion proteins) liposomes. Engineered, expressed and purified varied forms of SNARE proteins for use in the Surface Force Apparatus (SFA) system. Designed and engineered both a Cpx 1/Vamp 2 and a Cpx 3/Vamp 2, fusion constructs. Managed the laboratory Chemical/Biosafety and Radiation safety practices to meet compliance standards. Negotiated with vendor representatives and managed lab supplies and general equipment.Relevant Techniques:• Genetic Engineering for Recombinant Proteins• AKTA Prime-Low Pressure Liquid Chromatography System• Ion Exchange & Gel Filtration Chromatography/AKTA Purifier System• Liposome Reconstitution & Extrusion• Versed in Chemical/Biosafety & Radiation Safety Regulations

Expressed and purified various recombinant mammalian vesicle fusion proteins (SNAREs). Maintained antiserum stocks of polyclonal antibodies directed against said SNARE proteins. Studied the inhibition of intracellular vesicle transport using purified Fab fragments against Trans-Golgi Network SNARE proteins in the in-vitro Golgi Body transport assay. Isolated Golgi Organelle and cytosolic fractions from CHO wild type and VSV virus infected mutant CHO cells lines from large scale spinner flasks and cell factory cultures. Designed antigenic peptides for polyclonal antibody production in regards to Golgi SNARE proteins GS-15 and YKT6. Isolated an inducible stable cell line containing the Golgi SNARE, GOS-28/GFP construct in the Tet-ON/Tet-OFF system. Engineered and expressed constructs for synaptic SNARE binding protein Munc 13-1 for bacterial and Baculovirus expression systems.Relevant Techniques: • Recombinant Protein Expression & Purification• Affinity Purification of Antibodies• Immunoprecipitation• Fab Fragment Production & Purification• Antigenic Peptide Design• Mammalian Cell Transfection & Clonal Selection for Stable Cell lines• General Molecular Biology/Gene Cloning• Polymerase Chain Reaction• Primer Design for Genetic Manipulations• Protein Coupling & Labeling• Baculovirus Expression & Insect Cell Culture• Golgi Organelle/Cytosol Fractionation from CHO cells• VSV virus production• In-vitro Assay to Measure Golgi Vesicle Transport• SDS-PAGE• Western Blotting & Immunodetection

Purified T-lymphocytes from normal human blood samples using negative selection methods. Used T-Cell proliferation assays to study the stimulatory effects of lipopolysaccharides (LPS) on purified T-lymphocytes via the CD2 and CD3 activation pathways, and also to study the inhibitory properties of anti-CD18 antibodies on purified T-lymphocytes via the CD3 activation pathway. Isolated mRNA from LPS stimulated T-lymphocytes to determine levels of IL-2 transcription. Injected hybridoma cells into mice for monoclonal antibody production from ascites fluid. Determined antibody concentrations of hybridoma cell culture supernatants and ascites fluid using ELISA.Relevant Techniques:• Tissue Culture• T-lymphocyte Purification from human Peripheral Blood Mononuclear Cells• T-Cell Proliferation Assay• Immunofluorescence Staining• ELISA• RNA Isolation• Animal Work with BALB/c Mice and mouse colony maintenance

Bs, Biochemistry

Activities and Societies: American Chemical Society, Vice President of Alpha Phi Omega (National Service Fraternity), Amnesty International

Education record