William Eng Email & Phone Number

New York, NY

Greater New York City Area

Currently researching antibody based therapeutics to treat Acute Myeloid Leukemia.

Biomedical Chemistry Institute, Dept. Of Chemistry

Studied Galectin-3 (carbohydrate binding protein) related sorting of glycoproteins CD63 and Carcinoembryonic Antigen (CEA) and tetraspanin CD81 that are associated with Microvesicles/Exosomes from cell line SK-Mel-5. Used α-mannosidase I inhibitor, 1-deoxymannojirimycin (DMJ) in SK-Mel-5 cell culture in order to manipulate the glycan profile of the.

• Expressed and purified neuronal regulatory protein, Complexin (Cpx) 1 for cell based and liposome based fusion assays. Engineered and expressed constructs for protein Cpx 3 in the pET-SUMO vector system. Purified wild.
• Genetic Engineering for Recombinant Proteins
• AKTA Prime-Low Pressure Liquid Chromatography System
• Ion Exchange & Gel Filtration Chromatography/AKTA Purifier System
• Liposome Reconstitution & Extrusion
• Versed in Chemical/Biosafety & Radiation Safety Regulations

• Expressed and purified various recombinant mammalian vesicle fusion proteins (SNAREs). Maintained antiserum stocks of polyclonal antibodies directed against said SNARE proteins. Studied the inhibition of intracellular.
• Recombinant Protein Expression & Purification
• Affinity Purification of Antibodies
• Immunoprecipitation
• Fab Fragment Production & Purification
• Antigenic Peptide Design

• Purified T-lymphocytes from normal human blood samples using negative selection methods. Used T-Cell proliferation assays to study the stimulatory effects of lipopolysaccharides (LPS) on purified T-lymphocytes via the.
• Tissue Culture
• T-lymphocyte Purification from human Peripheral Blood Mononuclear Cells
• T-Cell Proliferation Assay
• Immunofluorescence Staining
• ELISA

Bs, Biochemistry

Activities and Societies: American Chemical Society, Vice President of Alpha Phi Omega (National Service Fraternity), Amnesty International

Education record