William Rosoff Email & Phone Number

Washington DC

• Determined cytoskeletal dynamics and contractile forces of neuronal growth cones in neurons of the peripheral and central nervous system
• Studied the distribution of focal adhesions in growth cones of the central and peripheral nervous system

Washington DC

• Developed novel culture system utilizing very thin mats (20-50 microns) of fluorescently label collagen
• Examined growth cone filopodia interactions with fluorescently labeled collagen fibrils, growth cone morphology and adhesion protein distribution between 2-D and 3-D culture

Queensland, Australia

• Designed and produced chemical gradients using a robotic fluid delivery device, the GeSiM Nano - Plotter
• Trained lab personnel in primary neuronal culture in 3 – D Collagen matrices and gradient production

Washington DC

• Established cell and neuronal culture experimental systems in the Dynamics Imaging Laboratory
• Examined differences of neuronal growth cone behavior in 2 – D monomeric, and 3 – D fibrillar collagen matrices using live cell confocal imaging

Queensland, Australia

• Further developed and optimized the axonal guidance assay
• Configured and programmed a robotic liquid delivery system (the GeSiM Nano – Plotter) for greater throughput
• Established culture and microscope facilities in the joint lab of Dr. Geoff Goodhill, and Dr. Linda Richards

Washington DC

• Established and supervised a Neuroscience wet lab counterpart to the lab of Computational Neuroscience
• Developed and implemented a novel assay for chemotaxis (Rosoff et al. Nat Neurosci. 2004)
• Generated controlled defined molecular gradients in 3D collagen gels
• Determined the molar concentration and gradient steepness required to guide axons
• The assay is applicable to any biological system in which a gradient of molecules is presented

Washington, DC

• Examined the regulated expression of Src kinases during muscle cell differentiation
• Determined trophic factors and signal transduction cascades that regulate myoblast maturation
• Measured activation of the Src kinases during myoblast differentiation and trophic factor treatment

Syracuse, NY

• Examined cytoskeletal and transmembrane proteins in differentiated myotubes
• Designed means for efficient transfection of myogenic cell lines
• Formulated coatings to support differentiation of contractile muscle cells on glass substrate

Stony Brook, NY

• Examined neurotransmitter clustering in muscle cells as a model for synaptogenesis
• Established a novel line of research by expressing exogenous rat brain glutamate receptors in muscle cells

Manhattan, NY

• Studied the regulation of plasma protein production in cultured primary and cell line derived hepatocytes
• Measured production of fibrinogen, hemopexin and other “acute phase” plasma proteins in chick primary hepatocytes

Manhattan, NY

• Examined cellular cholesterol metabolism and distribution in primary and cultured cell lines
• Measured accumulation of cholesterol esters as a model for foam cell formation in Atherosclerotic plaques

Doctor Of Philosophy (Ph.D.), Pharmacology/Neuroscience

B.A. Psychology (Minor Biology), Physiological Psychology, Perception, And Human Brain Function.