William-Ray Vista, B.Eng. Email & Phone Number

New York, Ny, Us

New York, New York, United States

Collaboration between the Interventional Radiology department (MSK) & the Adusmilli Laboratory (SKI)Funder: the NIH Research Project Grant Program (R01) & Department of Defense (DoD)In-Vitro Wet Laboratory Skills:• Electrical & thermal ablation treatments• Glioblastoma & mesothelioma establishment in mice brain & pleura• Transwell co-culture set up for blood-brain barrier model• 3D collagen gel & co-culture set up & maintenance• Live/Dead staining assays (Hoescht, Calcein, Propidium Iodide)• Trypan blue exclusion assay• Colorimetric cell viability & metabolic assays (CCK, MTT, MTS)• Luminescent & chromium release cytotoxicity assays• Clonogenic assays • Live & fixed stains (evans blue, crystal violet, nanoparticle, etc.)• Multi-color cell characterization via flow cytometryIn-Vivo Laboratory Skills:• Electrical ablation treatments• Small animal handling, identification, & necropsy• Small animal imaging• Organ harvesting, cellular isolation, & storing for paraffin embedding &H&EDry Laboratory Skills:• Scripting for single cell, colony, & viability analyses via MATLAB & Java • Masking, editing, & analyzing fluorescent, high-resolution cellular images via FIJI/ImageJ• Analyzing cytotoxicity between effector & target co-culture via IVIS• Flow cytometry gating via FCS Express & FlowJo• Basic graphing via GraphPad Prism• Manipulating and quantifying electrical delivery via circuitry & oscilloscope

Greater New York City Area

Radiology Department, The Stephen Solomon Laboratory • Serving as a principal investigator proxy for laboratory-related processes (ie. Institutional Biosafety Committee Management System, FDNY chemical safety and fitness, etc.)• Maintaining and operating basic cell culture related devices (electroporators, microscopes, plate readers, hoods, freezers, etc.)• Recording, storing, and preparing highly volatile or hazardous chemical/biological reagents and specimens • Supporting benchwork and literature publication for postdoctoral and research associates under PI: Dr. Etay Ziv

Schwartz Research Building, 1250 1St Avenue, New York, Ny 10065

Radiology Department, The Stephen Solomon Lab: Medical Research and Development (R&D)Funder: Society of Interventional Radiology Foundation• Carried out electroporation techniques onto oncological in-vitro and in-vivo models• Independently ran and maintained various chemotherapeutic reagent preparations, time-point treatments, and cell culture assays on various cell lines (Huh7, Mia-Paca, MB49, A549, etc.)• Produced 3D gel cell-infused scaffolds & cell-seeded permeable membrane inserts for pre-clincial & R&D purposes• Supported benchwork, animal model husbandry, and literature publication for international postdoctoral research fellows• Collaborated with various research cores & laboratories on clinical treatment, data acquisition, microscopic imaging, and cellular assays• Developed high-throughput oncological experimental designs under Principal Investigator: Dr. Govindarajan SrimathveeravalliWet Laboratory Techniques:• Live/Dead Staining Assays• Trypan Blue Exclusion Assays• Cellular Metabolic Assays (MTT)• Cellular Proliferation (CCK) Assays• Clonogenic Assays• Established glioblastoma in rat brain model• Prepared stains (evans blue, crystal violet, nanoparticle) for in-vitro and in-vivo permeability studies• Established & maintained transwell co-culture for in-vitro blood-brain barrier modelDry Laboratory Techniques:• Completed small animal necropsy & tissue harvesting/storing• Paraffin embedded tissue samples• Calibrated flourescent imaging and analyzed immunohistologically stained slides• Scripted ImageJ codes for colony forming and cell counting

Steinman Hall, 160 Convent Ave, New York, Ny 10031

The Barabino Laboratory, Vascular & Orthopaedic Tissue Engineering Research• Designed and fabricated multi-channel microfluidic systems via photolithography• Expanded and maintained suspension and adherent cell lines (K562, HuVEC, etc.)• Prepared and carried out chemical treatments on 3D cell-seeded microfluidic devices• ONCOLOGY: Investigated chemical factor influences on cancer cell lines toward erythrocytic lines for clinical and R&D purposes• TISSUE ENGINEERING: Recapitulated human vasculature via PDMS for testing properties and functionality of artificially synthesized bio-particles• Supported multiple Ph.D and post-doctorate biomedical engineers in research aims and experimental designsProcedures Operated:• PolyDiMethylSiloxane Molding• Corona & Plasma Treating• Benzidine Testing• Trypan Blue Exclusion Assay• Cell Mask, DAPI, & GEIMSA Staining

North Academic Center, 1605 Amsterdam Ave, New York, Ny 10031

Science, Technology, Engineering, and Mathematics (STEM) Tutor• Offered various tutoring services to college level students in the following courses: - General Chemistry I - Organic Chemistry I - Introduction to Psychology - Cognitive Psychology• Offered various tutoring services to high school level students in the following courses: - Environmental/Societal Chemistry - Algebra• Partnered with City College Academy for Professional Preparation & the Biology Department (BD) - Attended biology lectures to provide consistent reviewing materials - Coordinated study groups and 1-on-1 sessions - Collaborated with BD professors to create tutoring agendas - Created information materials from scratch (presentations, quizzes, and diagrams)

Steinman Hall, 160 Convent Ave, New York, Ny 10031

Summer STEM Institute (SSI) Program: Engineering Research• Introduced high school students to biomedical engineering (BME) cell culture, aseptic techniques, and various cell assays• Worked under a Ph.D student and guided students in performing various experimental protocols independently and interpret resultant data for optimization• Prepared and directed scientific findings via poster presentation at the end of program

Hamilton Grange Library, 503 W 145Th Street, New York, Ny 10031

Educational Services, One-to-One Tutor:• Mentored to high school and college level students in the following courses: - Algebra - General Chemistry I & II - General Biology I - U.S. Government and History

Dana Center, 510 East 73Rd Street, New York, Ny 10021

The Leon Root Motion Analysis Laboratory, Biomechanical & Musculoskeletal Engineering Research:• Assisted physical therapist in diagnosis and data collection of various patients with musculoskeletal diseases such as cerebral palsy, extremity deformity, etc. • Researched the quantification of core stability as a factor for various diseases and specific biomechanical diagnosis

Research Animal Resource Center (Rarc) & Institutional Animal Care And Use Committee (Iacuc), Research Scientist Training

Activities and Societies: Small & large animal husbandry, irradiation techniques, hazardous material and infectious agent usage, rodent.

Bachelor Of Engineering - Be, Bioengineering And Biomedical Engineering, 3.3

Minor in Chemistry

Bachelors Of Engineering (B.Eng), Biological/Medical Engineering, Cum Laude Graduate

Activities and Societies: Biomechanical/Musculoskeletal Research & Vascular/Orthopaedic Tissue Research Concentration"The Grove School’s.

High School Diploma, Science; Technology; Engineering & Mathematics (Stem)

Activities and Societies: National Honor Society, Jazz & School Band President, & Science Olympiad Team Competitor"Archbishop Molloy High.

Middle School Diploma, General Science & Mathematics

Activities and Societies: National Junior Honor Soceity"At HCJ we provide an environment of academic excellence where the program adheres.