William J. Fredericks Email & Phone Number

Luxembourg, Lu

Philadelphia, Pa, Us

★ Influenced the direction and progress of research to gain insights into the mechanisms of progression of urological cancers.★ Built a high performing team of skilled scientists and technicians engaged in a variety of laboratory functions.★ Designed and characterized plasmids, viruses, proteins, antibodies, and cell lines.CORE RESEARCH RESULTS► Led research into the loss of function of the tumor suppressor genes: the TERE1 (UBIAD1) prenyltransferase, and BAP1, an epigenetic regulator of chromatin, resulting in the publication of five papers and the acquisition of two research grants.► Characterized the BAP1-mediated dysregulation of metabolism in renal carcinoma.► Demonstrated the alteration of the metabolic phenotype in renal clear cell and bladder carcinoma cells via modulation of TERE1.☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲ABOUT THE UNIVERSITY OF PENNSYLVANIA SCHOOL OF MEDICINEThe Perelman School of Medicine, commonly known as Penn Med, is the medical school of the University of Pennsylvania. It is located in the University City section of Philadelphia. Founded in 1765, the Perelman School of Medicine is the oldest medical school in the United States. Today, it is a major center of biomedical research and education, and it is widely regarded as one of the country's top medical schools. Penn Med consistently ranks among the highest recipients of NIH research awards, and it currently holds the #5 spot on U.S. News & World Report's "Best Medical Schools: Research" list.

Philadelphia, Pa, Us

★ Launched a laboratory and associated tool set to lead the research into a gene of unknown function.★ Developed Adeno- and Lenti-virus for transduction of TERE1 and TBL2 cDNAs and MiRNAs to modulate gene expression.★ Applied for and secured an ACSIRG grant "TERE1 / TBL2: A novel protein complex with a role in cholesterol regulation and prostate cancer cell growth"​.CORE RESEARCH RESULTS► Demonstrated that natural TERE1 point mutations associated with Schnyder’s Corneal Dystrophy affect APOE binding and cellular cholesterol levels.► Applied two-hybrid analysis, mitochondrial co-localization, co-immunoprecipitation, GST association, and deletion/mutation analysis to validate interactions between the TERE1 and TBL2 proteins.► Validated the protein interaction between TERE1 and TIRC7, a T-cell regulator.☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲ABOUT THE UNIVERSITY OF PENNSYLVANIA SCHOOL OF MEDICINEThe Perelman School of Medicine, commonly known as Penn Med, is the medical school of the University of Pennsylvania. It is located in the University City section of Philadelphia. Founded in 1765, the Perelman School of Medicine is the oldest medical school in the United States. Today, it is a major center of biomedical research and education, and it is widely regarded as one of the country's top medical schools. Penn Med consistently ranks among the highest recipients of NIH research awards, and it currently holds the #5 spot on U.S. News & World Report's "Best Medical Schools: Research" list.

Philadelphia, Pa, Us

★ Managed a team of graduate students and post-doctoral researchers engaged in laboratory operations.★ Oversaw the process of regulatory submissions, grant production, and research paper writing, meeting publication schedules.★ Led a number of individual research projects, as outlined below.CORE RESEARCH RESULTS► Dissected the role of epigenetic co-regulators of transcriptional repression, which involved the siRNA-mediated expression silencing of KAP-1, HP1, and SETDB1 to target KRAB-mediated transcriptional repression.► Researched the role of SUMOylation and ubiquitinylation in KAP-1-mediated repression.► Created a KAP-1 co-activator expression plasmid to identify KRAB-Zinc Finger Protein target genes and evaluate the role of KAP-1 in cellular differentiation.► Produced a stable inducible BAP1 ubiquitin C-terminal hydrolase lung cancer cell lines to evaluate role in BRCA1-mediated TCR DNA repair and damage response.☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲ABOUT THE WISTAR INSTITUTEThe Wistar Institute is an international leader in biomedical research with special expertise in cancer research and vaccine development. Founded in 1892 as the first independent nonprofit biomedical research institute in the country, Wistar has long held the prestigious Cancer Center designation from the National Cancer Institute.

South San Francisco, Ca, Us

★ Directed breast cancer research studies.★ Developed inducible BRCA1 cell lines for assays of DNA damage response.★ Characterized BRCA1-associated proteins from yeast two-hybrid analysis.☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲☲ABOUT ONYX PHARMACEUTICALSBased in South San Francisco, California, Onyx Pharmaceuticals is an Amgen subsidiary, engaged in the development and commercialization of innovative therapies for improving the lives of people with cancer. At Onyx, we are grounded in three leadership hallmarks that guide how we manage our business and build value for patients and shareholders: innovation, patient-centeredness and stewardship.

Philadelphia, Pa, Us

• Analyzed transcriptional co-repressor function of KAP-1 (TIF1β)• Engineered inducible repressors to inhibit the malignant phenotype of alveolar rhabdomyosarcoma.

Philadelphia, Pa, Us

• Identified aberrant transcriptional activation by the oncogenic PAX3-FKHR fusion protein from the t(2;13) chromosomal translocation in Alveolar rhabdomyosarcoma• Targeted malignant phenotype suppression with a PAX3-KRAB transcription repressor• Characterized repression activity of the SNAG domain.• Demonstrated that the EWS-WT1 fusion from the t (11;22) translocation converts the WT1 repressor into a potent transcriptional activator• Prepared, purified, characterized non-cross reacting antisera for Pax3 and the Early Growth Response (EGR 1-4) family of transcription factors

Buffalo, Ny, Us

• Developed assays for analysis of multi drug resistance (MDR) gene, mRNA, and P-Glycoprotein (PGP) levels in 450 human tumors, normal tissues, and drug resistant cell lines.• Analyzed sarcoma patients’ medical database for association with PGP status

• Supervisor: Dr. R. Baker: Transfected genomic DNA from P-Glycoprotein-positive human sarcomas to mouse cell lines and selected for multidrug resistance phenotype• Supervisor: Dr. T. Beerman: Southern analysis of Dnase-hypersensitive actin gene domains to model DNA-reactive drug damage.

Laboratory of Dr Watt Webb• Immunofluorescence assessment of membrane protein /cytoskeletal interactions. • Cell surface electrophoresis / post-field relaxation of the LDL receptor in fibroblasts. • Membrane receptor / actin co-distribution during patching, capping of lymphocytes

Dr L. Glickman: ELISA survey of human serum samples for antibodies to Toxicara canis

Phd, Pharmacology And Experimental Therapeutics

Bs, Biology

Education record