Global Manufacturing Science and Technology (MSAT)

Study for cancer cell metabolism● Observed up-regulation of non-oxidative pentose phosphate pathway in cancer cells● Revealed the mechanism; glyceraldehyde 3-phospahte dehydrogenase modulates the anabolic activation and aerobic glycolysis using the methodologies of isotopic-tracing technology and molecular biology

● Metabolic flux analysis for global physiology of mammalian cell● Metabolic profiling: cell culture, metabolite extraction, derivatization and analysis with GC-MS● Isotopic tracer design: chasing metabolic fluxes using optimal isotopic tracers● Metabolic flux analysis (MFA): estimation of flux distribution within cell using labeling data of metabolites from mass spectrometry and computation● Model system: glycolysis system with CHO cells for bio-therapeutic production and gluconeogenesis with FAO cells and liver primary cells for diabetes and liver regeneration● I am developing mammalian cell models for each systems and experimental methodologies for MFA and studying cellular metabolism which can represent real metabolisms.● This technology can give a direction for cell line development and drug screening such as cell engineering by genetic manipulation and inhibition of specific target-enzymes.

Worked in biotechnology department to produce biologics and enzymes; human growth hormone, erythropoietin, bovine growth hormone and aminopeptidase[Fields] ● Recombinant DNA technology to express proteins ● Optimization of culture strategies with bacterial cell and mammalian cell; E.coli, B. subtilis. A. niger, S. ceresiviae and CHO cells using batch, fed-batch and perfusion culture modes● Purification skills; scouting columns and optimize good process for purification● Analyis of proteins; Maldi-Tof mass spectrometry

● Development of new folding process of bG-CSF produced by recombinant E.coli● Fermentation and purification of cholesterol lowering agents (HMG-CoA inhibitor) using mutant Aspergillus terreus

● Project on the commercialization of PHA supported by Procter & Gamble, USA - Investigated fermentation strategies for polyhydroxyalkanoate (PHA) production with Aeromonas hydrophila - Cloned the novel PHA synthesis genes from Aeromonas - Developed metabolically engineered recombinant E. coli for PHA production using Aeromonas PHA synthesis genes ● Project supported by Ministry Of Science and Technology, Republic of Korea - Produced polyhydroxybutyrate (PHB) from whey (by-product in dairy industry) using recombinant E. coli