Tsai Yi-Ting (Woofung) Email & Phone Number

Molecular biologist with a background in pathogenesis research and an interest in discovering potential genome editing therapeutics for human diseases.• Demonstrated leadership in training lab technicians as well as mentoring graduate students on genetics and stem cell projects.• Extensive hands-on experience in molecular biology techniques, including large construct design, BAC recombination, Gibson assembly/In-fusion/Traditional cloning, site-directed mutagenesis and qRT-PCR.• Proficient in genetic manipulation techniques, including more than 6 years of experience in CRISPR-based gene editing, CRISPR-based gene activation and silencing, Prime editing and base editing, protein over-expression, RNAi knockdown.• Experienced in systematic approaches including gene/protein expression profiling (microarray) for phenotyping mutations (mass spectrometry and RNA-seq), next generation sequencing for off-targeting analysis after gene editing, and protein interactome mapping (proteomics).• Developed protocols for immunology analytical methods such as flow cytometry (FACS), ELISA, cell-based phagocytosis assay.• Skilled in microscopy, including scanning electron microscope, LSM confocal, and live cell imaging. • Hands-on experience in ophthalmologic surgery on rodent animals.• Thrive in a team environment and work well with others.

Listed skills include Biotechnology, Virology, Molecular And Cellular Biology, Molecular Biology, and 14 others.