Research Assistant I
Baylor College Of Medicine - Breast Center
Houston, Texas, United States
- Conducted cell transient transfection and generated gene-knockdown stable cell lines using lentiviral vectors.- Performed and optimized antisense oligonucleotides (ASOs)-mediated exon-skipping of pre-mRNA in in-vitro cell culture.- Executed molecular cloning, semi-qPCR, and agarose gel electrophoresis to analyze alternative splicing events during the epithelial-to-mesenchymal transition (EMT) in in-vitro breast cancer cell culture.- Handled mouse genotyping and genotyping PCR to maintain lab mouse strains.