Advisor: Lance C. Kam• Led the study design and performed experiments using PBMC samples from chronic lymphocytic leukemia (CLL) patients, isolated T cells, and developed assays of T cell spreading and proliferation.• Developed and optimized multi-color imaging- and flow cytometry-based assays to characterize T cell function to understand how T cell behavior changes in CLL patients.• Applied feature-based machine learning model (Random Forest) and image-based deep learning model (Swin Transformer) to classify disease state and predict T cell proliferative capacity.• Filed a provisional patent as inventor aiming to accelerate immunotherapy through a rapid test of T cell health.• Awarded a BiomedX Ignition Grant totaling $25,000.Presentations:1. First-author poster titled Individualization of Immunotherapy by Mechanobiology and Machine Learning at 2022 Gordon Research Conference (GRC) for Immunoengineering.2. First-author poster titled Individualization of Immunotherapy by Mechanobiology and Machine Learning at 2022 Biomedical Engineering Society (BMES) annual meeting.3. Oral presentation as team lead at final pitch event of 2023 Nucleate Activator Program for BioEntrepreneurs based on Ph.D. research AI-Based Platform to Optimize T Cell Production for Personalized Immunotherapy.4. Oral presentation as team lead at 2023 Columbia Biomedical Technology Accelerator (BiomedX) Pitch Day based on Ph.D. research.

• Led the design and execution of luciferase-based cytotoxicity assay for Takeda mesothelin-targeted CAR-gamma delta T cell product characterization.• Performed RAS (repeat antigen stimulation) assay to test BCMA-targeted CAR-NK cell product's persistence of killing and analyzed data collected from Incucyte.• Supported gamma delta T transduction model development and bioreactor development

As a Technology Track Finalist and Team Lead in the Nucleate Activator Program for BioEntrepreneurs, I had the opportunity to immerse myself in the dynamic world of innovation, entrepreneurship, and biotechnology. This transformative experience allowed me to demonstrate my leadership, creativity, and passion for driving positive change in the life sciences industry.✨ Coordinated a Multidisciplinary Team: I led a cross-functional team. Together, we harnessed our collective strengths to tackle complex challenges in the biotech space.🚀 Innovation and Market Analysis: Through our collaborative efforts, we meticulously evaluated technical risks and market opportunities. This involved conducting in-depth interviews with industry experts, refining our value proposition, and designing a robust business model.🎤 Pitched to Prominent Audiences: As the designated spokesperson for our team, I had the privilege of presenting our innovative concept in front of an audience of more than 100 attendees, including esteemed judges and biotech experts. Our pitch highlighted the potential impact of our technology and our commitment to innovation.🌟 Mission-Driven Approach: We established a clear company mission and a robust Diversity, Equity & Inclusion statement, reflecting our commitment to creating a more inclusive and equitable future in the biotech industry.🔗 Networking and Collaborations: Throughout the program, I actively fostered connections with influential figures in the biotech ecosystem, paving the way for potential partnerships and collaborations.📈 Strategic Thinking: I played a pivotal role in crafting a comprehensive proposal for commercialization, which involved market analysis, IP strategy, and the development of a compelling pitch deck.

Advisor: Yingxiao (Peter) Wang•Engineered T cells with innovative ultrasound-inducible CAR construct to mitigate on-target off-tumor activity.•Led the design and execution of in vitro and in vivo potency assay.•Performed both subcutaneous injection and intraperitoneal injection in mice to implant tumors and test engineered CAR-T cells.•Quantified tumor area in mice by bioluminescence imaging.•Built and maintained PSMA-expressing prostate cancer cell line independently.•Harvested solid tumor from mice and characterized CAR T cell in the tumor.

• Worked on a project of automated sample preparation of microbial genome nextera for high-throughput sequencing with the platform at Shanghai ZJ Bio-Tech Co., Ltd.• Cooperated with the undergraduates from the Pennsylvania State University (SJTU-PSU Capstone Project).• Developed a real-time PCR kit which can be used to detect the high-throughput sequencing samples and the concentration

• Processed data of metabolites with Gas Chromotography-Mass Spectrometer and SIMCA-P.• Published an article entitled Metabonomics Approach to Comparing the Antistress Effects of Four Panax ginseng Components in Rats on Journal of Proteome Research as co-author.

Optimized a real-time PCR kit for quality control in the process of preparing a next generation sequencing (NGS) platform.

Advisor: Jordan Green• Worked on a project of polymeric particles for drug delivery.• Generated biomimetic ellipsoidal, discoidal and red blood cell-shaped poly (lactic-co-glycolic acid) (PLGA) particles.• Processed red blood cells and platelets to generate membrane-cloaked nanoparticles.• Delivered DNA intracellularly into dendritic cells using poly (beta-amino esters) (PBAE).

• Took four courses including Genetics, Foundamentals of Microbiology, Evolution & Human Diversity and Materials Science & Engineering.• Performed singing on "WorldFest" as representative of Chinese students.

• Worked on a project of psychophysical study of selective attention visual in Science & Rehabitation Engineering Laboratory.• Compared two methods of manipulating the size of the attention focus by adjusting pre-cue size and target spatial uncertainty.• Designed and carried out psychophysical experiments with Matlab Psychotoolbox.• Published an article entitled Using Spatial Uncertainty to Manipulate the Size of the Attention Focus on Scientific Reports as a co-author.

• Worked on a project of analysis of CpG islands methylation in SHP1 gene promoter region in Lung Cancer Cells Systems Biology and Medicine Laboratory.• Performed DNA extraction and modification.