Xinyue Chen Email & Phone Number

San Francisco, Ca, Us

San Francisco Bay Area

• Quantitatively characterized gene expression heterogeneity using single-cell RNA sequencing, single-molecule RNA-FISH, and flow cytometry.• Utilized targeted CRISPRi screens to uncover mechanisms by which small molecules regulate gene expression, identifying the base excision repair (BER) pathway as a key regulator of transcriptional noise.• Demonstrated the impact of gene expression fluctuations on facilitating cell-fate transitions during pluripotency induction and stem cell differentiation; investigated the role of transcriptional noise in cell-fate plasticity in the cancer EMT program.• Explored novel gene targets through a combination of computational modeling, gene expression analyses, and experimental validation.• Supervised and mentored research associates, guiding their research projects and supporting career development; managed lab microscopy equipment, including testing, maintenance, and personnel training.

New Haven, Connecticut, United States

• Designed and developed three inducible mouse models to study hematopoiesis and leukemia.• Developed an in vitro single-cell assay for tracking the transition of hematopoietic progenitor cells from normal to malignant states.• Identified and characterized key epigenetic regulators involved in the differentiation of hematopoietic cells toward myeloid and lymphoid lineages.• Analyzed cell cycle and gene expression heterogeneities in hematopoietic cells, assessing their roles in oncogenic responses.• Collaborated with bioinformaticians to conduct next-generation sequencing (NGS) analyses on hematopoiesis and leukemia.

New Haven, Connecticut, United States

• Advanced Topics in Cell Biology• Genes and Development• Ecology & Evolutionary Biology

• Differentiated iPSCs from Huntington’s Disease patients into GABAergic projection neurons.• Analyzed the stability of the huntingtin gene during iPSC reprogramming and subsequent neural differentiation.

Shanghai, China

• Contributed to the COMBREX project by functionally annotating prokaryotic proteins.• Expressed and purified proteins using column chromatography techniques.• Validated the kinase activities of purified proteins.

• Utilized zebrafish models to investigate folate metabolism and its link to congenital heart defects.• Mapped spatial and temporal gene expression patterns in zebrafish using RNA in situ hybridization.• Performed TALEN gene editing in zebrafish.

Doctor Of Philosophy - Phd, Cell Biology

Bachelor Of Science (B.S.), Biology/Biological Sciences, General

The International Asian Studies Program