LAB SKILLS: • Nucleic acid extraction (manual, automated using QIAcube, and Qiasymphony)• Nucleic acid quality check (NanoDrop1000, NanoDrop2000, Qubit 2.0, DNA 1000 & High Sensitivity chip using Agilent Bioanalyzer, KAPA 2 steps assay using Biorad Icycler)• Conventional PCR • Real-time PCR: Absolute quantitation using Taqman assays, KAPA SYBR® FAST qPCR Kits (Human Genomic DNA quantification), • Electrophoresis (gel & capillary)• DNA sequencing using Genetic Analyzer (ABI 310)• Pyrosequencing using PyroMark Q24• Primer Design (NCBI, Ensembl, IDT)• HPV detection using Hybrid Capture System• Samples processing for The Gallios Flow Cytometer• Pharmacogenomics testings (Copy Number on ViiA7, Open Array and Single Tube assays on Quantstudio 12k flex)• Single gene mutation detection (BRAF using Taqman assay on ViiA7, KRAS using Luminex 100/200TM)• Next gene sequencing (Ion One Touch 2, Ion PGM)• Toxicology set up and analyze (Shimadzu 8050 LCMS/MS)
Companiondx Labs
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Senior Molecular TechnologistCompaniondx Labs Jul 2013 - PresentHouston, Texas Area• Perform complex molecular biology techniques (DNA extraction, qPCR, RT-PCR, Next Gen Sequencing) • Prepare human biological material for genetic analysis. This may include blood, body fluids and tissues• Sets up phamarcogenomics testings (PGx), ananlyzes and reviews test data• Sets up, analyzes and reviews urine drug toxicology test data• Approves urine drug toxicology reports• Analyzes test results for acceptability against set controls and enters findings into the computer database• Operate complex equipment to prepare biologic specimens, to calibrate instrumentation, and to validate quality of assay. Perform cleaning, maintenance, alignment procedures, quality assurance assays, and troubleshooting on complex and testing equipment.• Maintain daily/weekly logs and QC documents for assays and equipment• Prepare stock solutions and reagents following standard operating procedures and quality control procedures. Record quality control of reagent preparation. Recognize and resolve problems or report to supervisor/manager.• Performing on all laboratory documentations: - Instrument qualifications - Reagent Lot Qualifications - Instrument maintenance logs - Parallel run documentation - Temperature logs• Perform proficiency testing to maintain compliance with internal and external regulatory agencies.• Help inventory maintenance by monitoring inventory levels of reagents and consumables, providing alerts to inventory coordinator if needed• Develop, training or assist in the development of clinical assays and the validation. • Supervising and training junior laboratory staff• Identifies and elevates Reportable Quality incidents to manager.• Maintain detailed, accurate and up-to-date documentation using our electronic LIMS system.• Preparing documents for CLIA, CAP and New York inspections
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Molecular TechnologistApplied Diagnostics Lab Dec 2012 - Jul 2013Houston, Texas Area• Perform complex molecular biology techniques (DNA extraction, QPCR, RT-PCR) under the direct supervision of the Laboratory Manager/Technical Supervisor.• Operate in accordance and compliance with all internal and external Technical Operating Procedures.• Analyzes test results for acceptability against set controls and enters findings into the computer database• Prepare stock solutions and reagents following standard operating procedures and quality control procedures. Record quality control of reagent preparation. Recognize and resolve problems or report to supervisor/manager.• Perform proficiency testing to maintain compliance with internal and external regulatory agencies
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RotationRotation At Baylor College Of Medicine In The Genomic And Rna Profiling Core Jun 2012 - Aug 2012Houston, Texas Area
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RotationRotation At Applied Diagnostics In Molecular, Flow Cytometry, And Cytogenetic May 2012 - Jun 2012Houston, Texas Area
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ResearchMd Anderson Cancer Center: Molecular Diagnostic Lab Sep 2011 - Feb 2012Houston, Texas AreaResearch: EML4-ALK fusion gene detection in lung cancer by novel ICEPlex PCR assay
Y Dinh, Mb(Ascp) Education Details
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Ut Md Anderson Cancer CenterMolecular Genetics
Frequently Asked Questions about Y Dinh, Mb(Ascp)
What company does Y Dinh, Mb(Ascp) work for?
Y Dinh, Mb(Ascp) works for Companiondx Labs
What is Y Dinh, Mb(Ascp)'s role at the current company?
Y Dinh, Mb(Ascp)'s current role is Senior Molecular Technologist at CompanionDx Labs.
What schools did Y Dinh, Mb(Ascp) attend?
Y Dinh, Mb(Ascp) attended Ut Md Anderson Cancer Center.
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