Ya-Ping Ko, Ph.D. Email and Phone Number

• Project leader and manager in studies of complement immune evasion of pathogen, identification of molecular basis of disease using biochemistry approaches, in vitro cell base assays, ex vivo host immune cells base assays (phagocytosis and flow cytometry). • Principal investigator in studies of Staphylococcus aureus exploitation of complement and coagulation systems. Studies had successfully secured for funding from Hamill foundation for 4 consecutive years. • Co-investigator in studies of S. aureus host-pathogen interactions, and a key data contributor to the resulting NIH RO1 funding since 2015, two patent applications and one awarded-patent. • Collaborative studies on human IgG responses to S. aureus infection and therapeutic value of these antibodies. • Collaborative studies on therapeutic antibody development using Phage display. • Skillful Cost and time management as demonstrated by overseeing and managing personal grants (>125k) over the 4 years period and a NIH grant ( >2.2 M) over the 5 year period in which I am a co- investigator. • Excellent presentation and communication skills as demonstrated by +5 presentations as invited speaker and +15 oral and poster presentations in international conference. I also supervised and trained students to do oral presentations and poster presentations for international and local conferences. • Serving as a co-chair on a graduate student’s PhD committees.

• Lead a team of graduate students and internship students to assess the pathogenicity of a gram-positive bacterium, identification and characterization of virulence factors using biochemistry approaches. • People management and interpersonal skill: Supervised and guided 3 graduate students and 4 international scholar/ internships students in performing experiments, tackling and trouble-shot problems, prioritize experiments and projects, analyzed and discussed experimental results who presented seminal results at local and international conference. • Highly self-motivated and innovative researcher implemented and extended research direction to complement biology and bacterial complement evasion strategies, resulting in integrating cross, 3 publications, and 1 patent application. • Demonstrated collaborative proficiency with experience in integrating cross-functional studies to peer-review research articles and 1 awarded patent.

• Lead a team of graduate students and internship students to assess the pathogenicity of a gram-positive bacterium, identification and characterization of virulence factors using biochemistry approaches. • Strong multi-tasking skills with experiences in managing a portfolio of projects including setting up in vivo animal model of diseases, maintain mouse husbandry, in vitro cell-base assays, as well as mentored 3 internships students. • Designed, executed and trouble-shoot the projects and managed the projects for publication.• Manuscript preparations and submissions.• Developed in vivo mouse model of infectious disease through route of i.v. and i.p.• Assisted in grant proposal preparation.• Assisted in reagent ordering and lab organization. Management of lab antibody bank by setting up a tracking system. • Developed independent research topic in innate immunity while managing the on-going projects.

• Supportive team player with experiences in assisting managing laboratory cell bank, antibody bank, bacterial strain bank and organization of reagents, as well as assisting with graduate student troubleshoot with their projects. • Assessed and characterized host-pathogen protein-protein interactions using biochemical and biophysics methods. • Designed and constructed gene of interest using molecular biology techniques, all aspects of protein expression, protein production and protein purification in E. coli and insect system, utilizing FPLC, Affinity chromatography and ion exchanger chromatography. • Developed animal model of S. aureus induced sepsis. Maintained and monitored mouse colonies. • Assisted in grant proposal preparation. • Supervise graduate students and undergraduate summer students.

PhD graduate student in International Graduate School in Genetics and Functional Genomic, University of Cologne, GermanyResearch Topic: Study the function of Extracellular Matrix protein in genetic knockout mice and siRNA knockdown Zebrafish. Dissertation title: Analysis of matrilin functions in knockout mice and knockdown zebrafish. • Project management: Designed, executed and prioritized the projects (mouse and zebrafish models), analyzed experimental data, keeping detailed records of procedure and documentation results for preparation of data for publications. This resulted in 2 first-author peer-reviewed research articles and 1 co-author research article.• Strong Strategic planning, Organization, Analytical and Problem solving skills as demonstrated by establishing zebrafish as an alternative model organism for studying the role of matrix protein in development. Provided regular care and maintenance of a zebrafish colony. The successful installation of the zebrafish facility and the model system had stimulated the international collaboration and had contributed fundamentally to 8 research articles and the system is still up in running. • Presentation and communication skill: Present research works in 3 international local conferences with wide range of audiences. Technical skills;Established Zebrafish model organism for research project, Zebrafish embryo microinjection. Zebrafish Husbandry, Maintain mouse colonies, Breed mice, mouse/zebrafish skeletal staining, prepare mouse samples for histology and immunohistochemistry studies. RNA isolation, Northern blot analysis and In situ Hybridization. Cartilage extraction, SDS-polyacrylamide get electrophoresis and immunoblotting, RT-PCR, whole mount immunostaining, gene mapping, primer design, recombinant protein expression in the mammalian system, protein purification, antibody purification and elimination of antibody cross-reactivity. Immunostaining on section.

Institute of Biomedical SciencesStudy tumor metastasis using in vivo mouse model and in vitro cell-base assays. Assessment of contribution of cell surface protein to tumor metastasis.• Performed Hybridoma technology to produce monoclonal antibody, hybridoma screening, cell based assay to select for stable hybridoma clone, ascites collection and antibody purification. • Executed mouse model of tumor metastasis, antibody therapy, and maintained mouse colonies. Handling SCID mice. • Planned, designed and executed molecular cloning of gene of interest, mammalian cell transfection, protein expression in mammalian cell. cell culture.• Performed cell based assays, transwell-cell base assays to characterize the correlation between the marker of interest and tumor metastasis.• Experience using flow cytometry/immunohistochemistry to measure immune cell activity in tumors.Technical skills;Mouse model of tumor metastasis, Hybridoma production, molecular biology, Cell culture, cell-base assays, in vitro trans-well assay, cell line culture, ELISA, sterilization technique, protein production in mammalian system, protein purification. Cancer Metastasis | Cancer Biology | Surface marker for tumor metastasis | animal tumor model | cell culture | Hybridoma mAb generation and production | Molecular biology.