Yana Gruber Email & Phone Number

United States

United States

Chemistry LabProcess Bureau of Marine Water Monitoring fresh and marine water samplesDetermining Nutrient Concentrations in Marine and Fresh water samples: NO3/NO2, NH3, Total-Nitrogen, Phosphorus, Total-PhosphorusAnalyzing Water samples for: Algal Levels, Dissolved Oxygen Levels, Total Suspended Solids, Alkalinity, Turbidity, Total organic carbon and dissolved organic carbon concentrationsVarious laboratory routines such as aliquotting samples, reagent prep, autoclaving various waste products, and maintenance of various instrumentationCreating and working off of Standard Operating ProceduresPerform water analysis in accordance with EPA&USGS methodologies

Rowan University; Research Advisor: Dr. Catherine Yang

Culturing of E. Coli to further induce plasmid activity with Isopropyl-β-D-thio-galactoside to produce Prostate Specific Antigen peptideExtracting Prostate Specific Antigen peptide using centrifugation and processing cells using various prepared buffers, concentrating the plasmid via dialysis, using a Liquid Chromotography Column to produce substance fractions, determining the concentration of the induced plasmid via Gel Electrophoresis and a MicrospectrophotometerFluorometric procedure determining inhibition of Prostate Specific Antigen by various chemical compoundsPurified small molecules such as peptides Created presentations with Microsoft PowerPoint.Wrote papers on research with Microsoft Word.Performed basic microbiology methods in an academic setting (e.g., transformations, cell culture, plate development).Extrapolated kinetic and thermodynamic data with UV-VIS from Lineweaver-Burk and Michaelis Menten plots and solved for the variables of Beer’s law.Determined the secondary structure of peptides and measured the extent of folding and unfolding of proteins upon the addition of denaturants using circular dichroismPerform assays for various compound testing including ELISAs, IEFs, SDS-Page, western blots, horizontal gels, pH testing, and UV-Vis.Majority of assays performed in vitro on large molecules such as proteins and immunoglobulins.Conducts qualification testing to support validations of new assays.

Prepared classrooms, within the Biology Department, for the students to conduct curriculum experiments. As well as preparing solutions, agars, cultures, various concentrations of acid and base solutions, preparing solutions with a specific pH, titrating solutions, and restocking classrooms with materials for experiments.

Preparing price quotes and contracts for potential clientsNetworking through various modes of communication to find new clientsMaintaining an organized and efficient office to better market deregulated Energy needs for corporate and small businessesAdvertising Informa Energy Inc. to the Northern Jersey, NYC region via mailings, and newspaper articlesElectronically cataloging business cards and other contact information into a database using Microsoft ExcelUpgraded all computers in the office to have the most up-to-date hardware and software as well as establishing a network for the home office