A promising mid level professional with experience of operating and working knowledge on a small and intermediate size bioreactors, and has significant knowledge of recombinant DNA technology, microbial cell culturing, maintaining bacterial cultures and protein purification. Has experience of working on various chromatography techniques appreciably FPLC, size exclusion and Ion exchange with proven ability to find the root cause of the problem, and find solutions to overhaul the hurdles such as cell growth inhibition, foam formation, and variation in retention time in columns that occur during cell culturing and purification stages.
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Research AssistantNjit Apr 2009 - Dec 2009Neural Signal Extraction/ Analysis- Assisted in data acquisition and processing of data with MATLAB.- Matlab code was written for analyzing EEG signals for detection of Alpha and Beta Frequency waves for study of their behavior during different states of consciousness and movements. -
Research AssociateNatco Pharma Ltd 2007 - 2008* Responsible for protein purification process development, Protein refolding, column packing, understand flow rate, pressure limitations, residence time and binding methods during purification scheme development.* Performing and optimizing concentration and dialysis operations using tangential flow filtration. * Demonstrated proficiency in FPLC, reverse phase, ion exchange, size exclusion, affinity chromatographic methods for protein purification and characterization, SDS-PAGE, Western blot.* Performed construction, isolation, quantified recombinant tDNA clones, gene cloning and sequence analysis, protein expression and purification, gene expression analysis, cDNA library construction, and PCR analysis.* Investigated abnormalities in protein extraction time. Determined root causes and corrected them by optimizing the processes.* Maintain records of experiments and report to Lab Manager
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Process EngineerBiological E Ltd 2006 - 2007*Production of Diphtheria, Pertussis and tetanus vaccine.* Ensure that cGMP processes are followed.* Maintain systems to comply with FDA regulations. * Microbial limit tests are conducted for analyzing the quality of water. * Samples are tested for the minimum lethal dose. * Maintaining standard cultures.* Diphtheria and Tetanus are tested for minimum reactive dose, detoxification, sterility, antigenic purity, formaldehyde and Thiomersol estimation, * Had to conduct sterility, potency and specific toxicity tests on the final product.
Yash Y Education Details
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Biomedical Engineering -
JntuBiotechnology
Frequently Asked Questions about Yash Y
What is Yash Y's role at the current company?
Yash Y's current role is Biotechnology Professional.
What schools did Yash Y attend?
Yash Y attended New Jersey Institute Of Technology, Jntu.
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