Yashvi Agarwal Email & Phone Number

Boston, MA, US

Boston, Massachusetts, United States

Cambridge, Massachusetts, United States

• Performing small-scale transient transfections in CHO cell line to express mutants designed to stabilize an enzyme fusion protein along with subsequent purification and characterization.
• Protein purification using various chromatography techniques: affinity, size exclusion, hydrophobic interaction, and ion-exchange on Bio-Rad and ÄKTA Pure machines.
• Protein characterization: analytical SEC (Waters AQUITY UPLC), spectrometry (Lunatic/NanoDrop), capillary electrophoresis (LabChip® GXII Touch), western blot, SDS PAGE.
• Transforming E. coli with DNA and subsequent plasmid purification (AmMag Quatro).
• Setting up large-scale cultures in vessels and assessing protein expression in cell supernatant (ForteBio Octet) for maximizing yield of protein.
• Preparing accurate dosing solutions for rat, mouse, and monkey studies after conducting bacterial endotoxin testing (Endosafe nexgen-PTS).

Massachusetts, United States

• Transfected HEK293T cell line to produce lentivirus and consequently performed viral transduction in Expi293F cell line to check for protein expression using flow cytometry and western blot.
• Maintained and passaged adherent and suspension cell lines like HEK293T and Expi293F.
• Used CRISPR/Cas9 to successfully produce gene knockouts in cell lines.
• Carried out cloning in E. coli using Gibson Assembly and restriction digest along with analysis using electrophoresis (FlashGel DNA System).
• Transformed E. coli, inoculated culture and optimized DNA extraction using various mini, midi, and maxiprep kits.
• Ensured smooth deliveries of plasmids from CRO to scientists and maintained accurate electronic records.

• Worked on inhibiting P-glycoprotein using gold nanoparticles conjugated with verapamil and R6G to treat multidrug-resistant triple negative breast cancer cell line. - Carried out cell culture of the MDA-MB 231 cell.
• Conducted an enzyme kinetics experiment to distinguish between a competitive and uncompetitive inhibitor of alkaline phosphatase.

Boston, Massachusetts, United States

• Prepared microfluidic devices for in-situ droplet based analysis of immunogenetic microtumors.
• Performed cell counting and passaging of patient-derived cancer cell lines.
• Assisted in fluorescence microscopy analysis using Zen microscopy imaging software.

Mumbai, Maharashtra, India

• Formulated probiotic tablets using polysaccharide and various coating methods
• Research for existing formulations in the market and prepared a report.

Mumbai, Maharashtra

• Dr. Vandana Patravale's Laboratory
• Researched about topics to provide a solution to the Industry Defined Problems.
• Conducted literature research for synthetic and natural polymers to make an effective waterless hand-cleaner.
• Hypothesised a theoretical formula for the product and make test batches in the laboratory. This internship has taught me how to conduct in-depth research about topics and implement them practically. I also learned to.

India

• Ensured smooth functioning of the WordPress website by creating new events at the back end using the information provided by the clients
• Posted content 3-5 times a week on Instagram and Facebook to increase brand visibility
• Made at least 50 calls a day and persuaded them to list their business on the website
• Created Facebook ads to increase ticket sales

Masters In Pharmaceutical Sciences, Pharmaceutical Sciences, 3.9

Courses: •Human Physiology and Pathophysiology •Concepts in Pharmaceutical Sciences •Pharmaceutical Sciences Laboratory •Professional.

Bachelor'S Degree, Pharmacy, 3.98

Activities and Societies: •Director of Editorials and Content Curation at TEDxICTMumbai •Head of Media Marketing and Outreach at Manzar.

High School, 92%

Igcse, 92%