Yuan-Cho Lee Email & Phone Number

Lawrence Township, NJ, US

• Project: Developed and character monoclonal antibodies
• Generating plasmids for recombinant monoclonal antibodies.
• Analyzing data characterizing the integrity, integration number, copy number, transcript quality, and variants from the expression cassette used to generate cell lines of interest.
• Library construction, DNA/RNA next generation sequencing
• Improve the processes for constructing and enhancing cell line performance for CLD.

• Project: Developed monoclonal antibodies
• Experienced in fed-batch and cell culture processes development to select canonized monoclonal antibodies.
• Implemented ELISA, or AlphaLISA in Baf3 and DH82 cells for tittering the activity of antibodies
• Validated and optimized existing assay protocols to drive performance
• TA cloning method for sequencing canonized monoclonal antibodies

New York, New York, US

• Project: Study the function of HR DNA repair factors by using CRISPR method.
• Conduct focused research on the identification of significant functional HR repair factors in breast cancer, such BRCA1, BRCA2, and the MRN complex.
• Implement protein-protein interaction assays to identify the factors under study, such as the co-immunoprecipitation and tandem affinity purification (TAP).
• Research goal is to generate essential findings for understanding the etiology of breast cancer, as well as create positive implications for the clinical management of breast cancer.

New York, NY, US

• Project: Recognize and repair DNA lesions by mammalian nucleotide excision repair (NER) proteins.
• Led analysis of the effects of human XPC and the yeast homolog Rad4 NER proteins in the recognition of DNA lesions, focusing on the ability of XPC proteins to recognize a variety of stereoisomeric bulky PAH-derived DNA.
• Determined DNA sequences and protein mass by MALDI mass spectrometry.
• Applied DNA ligation and isolation technique to synthesize DNA oligonucleotides containing lesions.
• Developed binding affinity assay to study recognition mechanism of Nucleotide Excision Repair (NER) proteins.
• Utilized EMSA to detect DNA-protein interaction.

New York, NY, US

• Project: Mechanisms of translesion synthesized catalyzed by the Y-family polymerase Dpo4.
• Investigated effects of DNA template base sequence on error-prone and error-free translesion synthesis catalyzed by replicative and Y-family polymerases.
• Used Sanger sequencing methodologies to evaluate stereoisomeric bulky benzo[a]pyrene metabolite-derived guanine and adenine lesions.

• Employed an event-specific PCR system to detect the Genome Modified Maize line.
• Identified the percentage of GM corn in animal food by designing a real-time PCR system enabling the quantification of GM maize content.

Taichung, TW

• Project: Use of the random amplified polymorphic DNA assay (RAPD) to detect sodium arsenite-induced DNA damage in human lymphoblastoid cells.
• Utilized the RAPD assay to identify DNA base alterations and employed PCR analyses to screen random primers.
• Purified RPAD assay sequences and ensured result accuracy through the use of Northern blotting and RT-PCR.

TW

• Project: Apoptosis in human cells induced by different anti-cancer drugs.
• Determined effects of various anti-cancer drugs on apoptosis in cell lines.
• Detected cell survival rate with the use of viability assay and variance in drug concentrations.
• Applied fluorescent DNA-binding dye (DAPI) to identify morphology changes in apoptotic nuclei and Western blotting techniques to analyze apoptosis protein expression.

Certificate, Advanced Business

Doctor Of Philosophy (Phd), Molecular Biochemistry

Master Of Science (M.S.), Biology, General

Bachelor Of Science (B.S.), Biology/Biological Sciences, General