Ying Zhang Email and Phone Number

•Characterized degradants in native and stressed biologics drug substances and products using Agilent HPLC including size exclusion chromatography and reverse phase, and micro fluidic Lab-on-Chip technology•Conducted forced degradation for comparability study in a very tight timeframe from initiation of stresses to technical report publication in only 7 weeks•Lead forced degradation for probing, comparability and similarity studies on biologics drug substances and products•Characterized impurities isolated from native and stressed biologics substances using analytical methods

•Characterized degradants in native and stressed biologics drug substances and products using Agilent HPLC including size exclusion chromatography and reverse phase, and micro fluidic Lab-on-Chip technology•Conducted forced degradation for comparability study in a very tight timeframe from initiation of stresses to technical report publication in only 7 weeks•Published more than 10 technical reports and protocols for forced degradation studies and analytical method development•Lead forced degradation for probing, comparability and similarity studies on biologics drug substances and products•Characterized impurities isolated from native and stressed biologics substances using analytical methods •Developed fit-for-use ELISA method for detection of host cell protein in drug substances and drug products•Characterized Fab-arm exchange between two IgG4 molecules•Participated in the method development to characterize host cell protein assay using silver staining, 2D-DIGE and Western Blot

•proteins purification and crystallizations leading high quality x-ray datasets of apo proteins and protein/ligand complexes to support Structure Biology projects•Extensive experiences in AKTA chromatography system (FPLC, HPLC) with affinity columns (nickel, cobalt), ion-exchange columns (IEC), size exclusion columns (SEC), desalting columns and HIC •Experiences in analytical separation (HPLC, UPLC) and characterization techniques (LC-MS)•methods development for protein purification and crystallization•Implemented and maintained automation systems for automated crystallization setup and crystal imaging•Managed group laboratory supplies, inventory and records; maintained laboratory equipments

•Gene expression, protein purification and x-ray data collection to support structure biology projects•Constructed novel vectors for protein expression in E. coli. using Vector NTI for construct design •Implemented automation systems (Tecan Liquid Handling system 96+1) for crystallization screening

•Supported several NIH-funded projects with protein expression and purification for crystallization •Designed and cloned genes involved in RNA splicing, transcriptional silencing and DNA replication using cDNA libraries from different species (bacterial, yeast and human) •Championed large-scale preparation of palindrome DNA fragments for reconstitution of symmetrical nucleosome core particles

•Contributed to the myosin light chain phosphatase project (NIH AR21673): •Cloned three subunits of myosin light chain phosphatase from chicken gizzard using RT-PCR •Discovered a novel isoform of the 20 kDa subunit predominant in chicken gizzard