Yi Liu Email & Phone Number

Bethesda, Md, Us

Bethesda, Md, Us

Gaithersburg, Maryland, United States

• Lead cell line development projects on early-stage vaccine development in CHO-DG44 cells and HEK293 cells.

Gaithersburg, Md

• Lead cell line development projects for the expression of bispecific antibodies in CHO-GS system.• Worked closely with upstream, downstream, analytical and research groups for process development, material transfer, data analysis and trouble shooting.

Bethesda, Md

• Identified a novel tumor suppressor and prognostic serum biomarker for adrenocortical tumors through genome-wide analysis, mammalian cell culture and mouse xenograft models.• Coordinated a Phase I clinical trial using a recombinant cytotoxin for the treatment of metastatic adrenocortical carcinoma. Managed clinical sample collection and testing, evaluated drug safety and pharmacokinetics. • Established collaborations with scientists within and beyond NIH in assay development and integrated genome-wide data analysis.• Served as a technical lead in areas such as stable cell line development, immunohistochemistry, immunofluorescence staining, bright field and fluorescence microscopy, DNA/RNA assay development, and protein analysis.• Frequent project presentation in small and large conferences.

Baltimore, Maryland Area

• Characterized the tumor suppressive role of a novel component of the emerging Hippo tumor suppressor signaling pathway via cell culture and genetically modified mouse models.

Baltimore, Maryland Area

• Developed cell-based assays, molecular and biochemical assays in measuring protein-protein interaction and cell signaling transduction, identified a novel transcription factor for the Hippo tumor suppressor signaling pathway. • Identified a critical protein-protein interaction that can be used as drug target for tumor suppression.• Developed and characterized four transgenic mouse models, and 20+ stable cell lines.• Designed and implemented adenovirus and baculovirus-mediated protein expression system in cultured mammalian and insect cells.• Managed lab inventory, ordered equipment and supplies, established standard operating protocols for laboratory experiments. • Established technical protocols, and trained laboratory technicians and junior-level students in performing regular lab tasks.

Frederick, Md

• Performed small-scale process development studies for cultured mammalian cells.• Developed effective clone picking strategy to identify clones suitable for large-scale protein production.• Knowledge of CGMPs and SOPs.

Hefei, Anhui, China

• Developed monoclonal anti-ErbB2 antibody using hybridoma techniques.• Participated in chimeric anti-ErbB-2 antibody engineering and expression in CHO cells.• Performed small and medium-scale CHO cell culture for antibody production.

Ph.D., Biochemistry, Cellular And Molecular Biology

B.S., Biological Sciences