Young-Min Soh Email & Phone Number

Gaithersburg, Maryland, United States

Maryland, United States

Structural Cell Biology Lab• Purification of Integrin (membrane protein complex) from human placenta• Protein expression in HEK293S suspension culture• Purification of different mutant proteins for ELISA and Cryo Electron Microscopy (CryoEM) studies• Training, teaching, and acting direct supervisor of post-baccalaureate students• Teaching and training students on lab techniques and how to properly use/maintain Akta Pure machine• Advising on experimental design and troubleshooting with results• Maintaining and coordinating preparation of competent cells (BL21, XL1 Blue, pIRHC, DH5alpha)• Lab expert in molecular cloning techniques (Gibson Assembly, Golden Gate Shuffling, conventional restriction/ligation)

Lausanne, Switzerland

Chromosome Organization and Dynamics• Discovered a novel enzymatic function, which was hidden for decades, of the chromosome organizer, ParB• Characterized the enzyme kinetics of ParB CTP hydrolase activity• Solved the crystal structure of ParB bound to ligand• Designed novel assays based on chemical cross-linking in order to recapitulate ParB loading onto DNA• Results were published in the journal Science (DOI: 10.1126/science.aay3965)• Article gained F1000 recommendations• Instructed and provided guidance for first year Master Student (Caroline Gaille) Oct. - Dec. 2018• Explained and aided in designing constructs (protein expression), making the constructs, and expressing protein

Planegg, Bavaria, Germany

Chromosome Organization and Dynamics• Discovered an alternate conformation of a DNA damage control protein, Rad50• Constructed plasmids, for protein overexpression in insect cells, that are compatible for Golden Gate shuffling• In vivo cross-linking proteins in Yeast• Chemical crosslinking for validating crystal structures in vitro

Doctor Of Philosophy (Ph.D.), Biology (Structural Biology)

Bachelor Of Science (B.S.), Biology