Yu (Patrick) Shi, Phd. Email & Phone Number

San Diego, CA, US

San Diego, California, United States

South San Francisco

- Extensive experience with advance patch-clamp techniques (e.g. whole-cell, cell-attached, inside-out, outside-out configurations) to support multidisciplinary drug discovery programs.- Strong knowledge and experience with ion channel biophysics, electrophysiology, and molecular biology.

Stanford

- Investigated the gating mechanism of the store-operated calcium channel Orai by characterizing mutations using classic patch-clamp configurations (e.g. whole-cell, inside-out, and outside-out)- Performed routine site-directed mutagenesis, gibson cloning, primer designing, and DNA purification- Experience with routine cell culture on various cell lines.

Burnaby Canada

- Extensive experience in cardiac cellular physiology in the context of cardiovascular diseases (e.g. Long QT syndrome, myocardial infarction, and cardiac arrhythmia)- Studied and published four first-author peer-reviewed articles on the mechanism of action of external protons on the hERG channel activation and deactivation process using a combination of.

Simon Fraser University

- Performed cryosection using the Leica Cryostat for sample preparation in immunohistochemistry experiments- Extensive experience and knowledge in immunohistochemistry- Investigated CD4+ and CD8+ T-cells response to absence/presence of BIM in transplant vasculopathy. Data collected and analyzed in this honour project resulted in a publication.

Lethbridge

- Extensive experience working with bacterial culture, gene knockout, ELISA, electroporation and PCR- Investigated and discovered a promoter region responsible for toxin level difference between two different strains of E.Coli O157:H7.- Designed, constructed, and named a plasmid: pSHI01, to be used for gene knockout experiments in the future.

Burnaby Canada

- Implemented and optimized the protocols for site-directed mutagensis, PCR, gel electrophoresis, bacterial culture, and RNA extraction- Created a library of mutations (>50) in hERG potassium channels, and sodium channels

Doctor Of Philosophy - Phd, Biological And Biomedical Sciences, 4.0

Bachelor Of Science (B.Sc.) (Honours), Molecular Biology And Biochemistry